ラットに

3H

-CU-

83

(S)を25μg/kgで静脈内あるいは経口投与し，血液中濃度および尿糞中排泄を検討した．
静脈内投与後の血液中濃度推移は投与後5分より上昇し，投与後45分に25.74ng eq./mlのC_maxを示し，それ以後t1/

2

3

.05時間とt1/

2

33.09時間の二相で減少した．投与後72時間までのAUCは135.42ng eq.·hr/mlであった．
経口投与では，投与後

3

時間でC_max 4.10ng eq./mlに達し，以後t1/

2α

4.46時間とt1/

2β

26.

83

時間の二相で減少した．投与後72時間までのAUCは48.62ng eq.·hr/mlであった．
静脈内投与と経口投与のいずれの場合も，尿および糞中への放射能の排泄は，投与後48時間でほぼ終了した．静脈内投与では，投与後72時間までに投与量の30.52%が尿中に，60.42%が糞中に排泄された．経口投与では，同じく72時間までに40.34%が尿中に，69.24%が糞中に排泄された．

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The acetone extracts from three samples of the hepatopancreas of the poisonous scallops obtained on the Okhotsk Coast of Hokkaido Island were fractionated into two parts, hexane soluble fraction (fraction H) and 85% aqueous ethanol soluble fraction (fraction ) by partition to two layers. The majortoxic components in the mouse assay of “diarrheic shellfish toxin” by intra-peritoneal injection were found to be free unsaturated fatty acids showed the following toxicity in MU per g, 18:1 n- 35, 18: n-6 , 18: n- 167, 18:4 n- , 20:5 n- 167, and :6 n- , respectively. Toxicity of the fraction Hin MUper g was much lower than that of the fraction . However, the toxicity of the fraction H per 1 g of the hepatopancreas was about twice that of the fraction , since the fraction Hwas much more abundant than the fraction in the hepatopancreas. The method for the assay of the diarrhetic shellfish toxin must be reexamined by considering the toxic effect of the free unsaturated fatty acids.

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A structure analysis of has been carried out to determine the deuterium trap sites by neutron powder diffraction with the Rietveld profile analysis. It is revealed that the deuterium atoms are located at octahedral () sites surrounded by six Ti atoms in the crystal structure of , space group P6₃/mcm. Local vibration spectra of hydrogen in measured by neutron inelastic scattering support this result; the energy eigenvalue of the primary vibration mode is found at 7.53 kJ/mol (78 meV). The hole radius and the spring constant of the Ti-H(D) bond are discussed.

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The EISCAT Svalbard Radar (ESR) has been used to obtain ion velocities in the lower thermosphere. By using beam swinging and assuming homogeneity and stationarity of the plasma, first approximations to the electric field have been deduced, and thus the thermospheric neutral wind has been estimated. From these derived parameters, we have estimated the gradient Richardson Number. Although many assumptions must be made, there is an indication that electrodynamics is able to contribute to enhancement or even production of neutral-air turbulence in the lower thermosphere. Finally, we outline a proposal for an analogy to the Reynolds Number, but reflecting the relative importance's of the contribution of ion-drag to the neutral dynamics and the kinematic viscosity.

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The numerical values of the intervals betrween optieal levels are competed for the configurations *⁹4s, 5s, 6s and 7s of Cu⁺, according to the general expression of energy-levels derived in the previous paper The self-consistent field radial functions computed by Hartree adn Hartree are used for1s, s, p, s, p and d. Those of 4s, 5s, .s and are ealenlated from Hartree Hartree's core-functions by the numerical integrations. The calculated results are shown in Table I.The agreement with experiment is satisfactory

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本検討は, 経皮的冠動脈形成術 (PCI) 後の翌日血液透析 (HD) 時における透析中低血圧 (IDH) の発生因子について検討した. 対象は待機的PCI後, 翌日HDを施行した慢性HD患者連続

名 (年齢70±7歳, 男性64名). IDHの定義は, 収縮期血圧20mmHg以上の低下で, 症状を伴いかつHD中断を要したものとした. また, HD記録よりIDHを後方視的に調査し, Logistic回帰分析にてIDHの予測因子を検討した. IDH群 (12名) は非IDH群 (71名) に比して, 有意に低体重 (52.0±. vs. 62.±1.5kg; p=0.007), 造影剤量/体重が多く (.5±0. vs. 1.±0.1mL/kg; p=0.018), 低左室駆出率で (45.±4. vs. 56.0±1.% ; p=0.030), /’ が高値 (.±. vs. 17.±0.cm/sec; p=0.038) であった. また, 冠動脈病変や侵襲的な治療について有意差は認めなかった. Logistic回帰分析では, 造影剤量/体重 (OR, 6.87; 95%CI, 1.-25.; p=0.004) がIDHの有意な予測因子であった. 造影剤使用量が多い症例は, IDHを生じる危険性が高いことが示唆された.

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The percentages of germination of seeds, dyad cells, good pollen grains and the fcrtility of piants obtained by crossing between varieties of Abel. esculerdu's and I variety of Abel. Manihot wcre different with the varieties of the mother plant, ranging ca..7%-.%, ca. .7 %-99.%, ca. 28.%-90.% and ca. 0.00-0. per pod respectively. The strains showing the good percentage in the gerrnination of seeds were also superior in the other characters, while those tnat werepoor in the germination were also inferior in the other characters.

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The thermo-isomerization of l-cis-pinane (1), -pinene () and (10)-pinene () in the presence of synthetic zeolites (A-, A-4, A-5, - and TSZ-642) was investigated. Whith TSZ-642 zeolite, dihydromyrcene (5) was obtained with high selec-tivity (high 85%) in all converted products from (1). 4 /6 Z-Alloocimene (10) and 4 /6 -alloo-cimene (11) were obtained from () as the main products, amounting to 86% (77:) of the isom-erization product. Myrcene (12) was obtained from () as the main product, amounting to % of the isomerization product under the best conditions.

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性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-AおよびType203で測定した結果,励起光538nm,螢光552.5nmであった。この螢光特性は, およびにそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•5±•5nmを読み,ALLENの補正を行なった。この条件において,および-methyletherの最少検出量は1ngであった。回収率補正の目的で加えた6,7-

3H

-

E₂

-17βの全過程における回収率は平均60.

3

±11.7%であった。正常性周期を示す黒毛和種

2

頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共

E₁

,

E₂

の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,

E₂

は

E₁

にくらべ全般に高値であった。また

E₃

は検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.

3

および

99.8ng

/l;

E₁5.9

および16.0ng/l,

E₂29.4

および

83.8ng

/l)を形成し,排卵後は急激に減少して最低値(

3

.

8

~

5.3ng

/l;

E₁1.6

および

1.9ng

/l,

E₂2.2

および

3.4ng

/l)を示した。黄体期の最高値(10.1および27.0ng/l;

E₁2.4

および

3.4ng

/l,

E₂7.7

および23.6ng/l)は排卵後6~

8

日に認めた。すなわちestrogenの血中濃度は性周期の間に

2

つのピークを形成することを認めた。

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1991年～2005年の福井県など4県における散発下痢症患者由来の大腸菌0153107株について, 市販の薬剤感受性ディスクを用いたKB (Kirby-Bauer) 法で12剤の薬剤感受性を調べた. 薬剤別耐性菌出現率はampicillinが72.%, streptomycinが48.6%, tetracyclinおよびsulfisoxazoleが46.7%, nalidixic acid (NA) が29.%およびciprofloxacin (CPFX) が24.%などであった. 7～10剤に耐性を示す18株中16株など計26株が, NAおよびCPFXに耐性を示した. NAおよびCPFXに耐性を示した24株とNAに耐性を示した1株について, gyrAおよびparC遺伝子の解析を行った結果, 次の4typesに分けられた. type1 (1株) GyrA (SL) ・ParC (S80I), type (12株) GyrA (SL & D87N) ・ParC (S80I), type (株) GyrA (SL & D87N) ・ParC (S80I & 84G) または (S80R & 84V), type4 (4株) GyrA (SL & D87N) ・ParC (S80I & A108T). アミノ酸変異とfluoroquinolone (FQ) 系薬剤の最小発育阻止濃度 (MIC) との関連をみると, CPFX, ofioxacinおよびnorfloxacinのMICはtype1では, それぞれ1μg/mL, μg/mLおよびμg/mL, typeでは～32μg/mL, ～32μg/mLおよび16～256μg/mL, type, 4では32～256μg/mL, 32～128μg/mLおよび128～>512μg/mLであった. 患者由来のFQ系剤耐性大腸菌O153が多剤耐性傾向を示すとともに, gyrAおよびparCで各々1～カ所の変異がみられた.

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A Fabry-Perot resonance (.P.R.) type Faraday rotator which consists of a magneto-optic medium with mirror coating at the ends has been made. The Faraday rotation of the medium is enhanced (1+Re^-αl)/(1−Re^-αl) times (R: reflectivity of the mirror, -αl: optical loss of the medium) under .P.R. condition. As a theory predicts, the Faraday rotations of the rotators using Pyrex glass and GGG with silver coating of R=0. and 0. were enhanced 10 and 20 times, respectively, while, when the rotator using LPE-garnet film with silver coating of R=0. was used, the enhancement was limited to times. The difference comes from the effect of the optical absorption -αl.

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妊娠ラットと授乳中の母獣に

3H

-CU-

83

を25μg/kg経口投与し，胎盤通過性および乳汁中への移行性を検討した．また，雄ラットに

3H

-CU-

83

を25μg/kgで1日1回で7日間あるいは14日間連続経口投与した際の体内動態を単回投与の結果と比較検討した．
妊娠19日の雌ラットに経口投与した時の母獣の組織内放射能濃度で高い値を示したのは肝臓と腎臓であったが，それぞれ血漿中濃度の約

2

.4倍と約1.

9

倍であった．胎仔の組織内濃度は投与後6時間に最高値を示したが，いずれも母獣に比べ極めて低い値であり，CU-

83

およびその代謝物は胎盤を極めて通過しにくいことが示された．
分娩後の授乳ラットに経口投与した場合，乳汁中放射能濃度は投与後

2

時間に最高値を示し減少した．しかし，血液中濃度と比較して，その濃度比は0.23～0.72と低く，乳汁中への移行は小さいことが明らかとなった．

2H

-CU-

83

を25μg/kgで1日1回，単回投与と7日間あるいは14日間連続経口投与した時の血液中濃度推移，組織分布および尿，糞中排泄を検討した．7日あるいは14日間連続投与の最終投与後の血液中濃度推移は単回投与の結果と差がなかった．毎回投与後24時間の血液中濃度は投与後5日まで上昇し，その後は一定となる傾向を示した．組織内濃度は，単回投与に比べ7日間投与では増加が見られたが，7日間と14日間投与ではほぽ同じ濃度であった．また，尿および糞中への放射能の排泄は，最終投与後24時間までにほぼ終了した．以上より，本化合物の生体への蓄積性は極めて低く，連続投与しても体内に残留しないと考えられる．

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A structure analysis of has been carried out to determine the deuterium trap sites by neutron powder diffraction with the Rietveld profile analysis. It is revealed that the deuterium atoms are located at octahedral (b) sites surrounded by six Ti atoms in the crystal structure of , space group P6₃/mcm. Local vibration spectrum of hydrogen in measured by neutron inelastic scattering supports this result; the energy eigenvalue of the primary vibration mode is found at 7.53 kJ/mol (78 meV). The hole radius and the spring constant of the Ti–H(D) bond are discussed.

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Mace- extract obtained from seed covering of nutmeg is expected to show an antiarteriosclerotic effect due to its potentiation with unsaturated fatty acid, various vitamins, etc. In this study an effect of Mace- was examined from serum lipid and six constituents of thoracic aorta.
A total of 16 male rabbits of 38 months, including with the Mcce- (M group), 4 with cholesterol (C group) and 4 with Mace- plus cholesterol (MC group) were used. to produce arterosclerosis cholesterol was orally given at 1g to the C and MC group. To the M and MC groups Mace- was given at 500mg/day. Sixteen weeks later referring to all the rabbits, total cholesterol (T. CHO), triglyceride (TRIG), β-lipoprotein (β-LIPO), HDL-cholesterol (HDL), 4 in total of serum lipids, were measured. Furthermore, all the rabbits were killed and constituents media of thoracic aorta were stained and observed histochemically, i. ., SMC with Azocarmine G stain, EL with Weigert's stain, CL with Van Gieson's stain, AMPS with Alcian blue stain and GP with PAS stain. T. CHO was 90.5mg/dl in M group, 369.mg/dl in C group and 81.mg/dl in MC group respectively. The MC group showed no difference with M group but the significantly lower value than that of C group (p<0.01). TRIG was 108.5mg/dl in M group, .mg/dl in C group and 94.4mg/dl in MC group respectively. No significant difference was observed among these groups. Beta-LIPO was 184.mg/dl in M group, 995.mg/dl in C group and 151.5mg/dl in MC group respectively. Compared with C group, M and MC groups showed significantly low value (p<0.001-p<0.01). HDL was 18.mg/dl in M group, .mg/dl in C group and 26.5mg/dl in MC group respectively. There was no significant difference among those group, although it was highest in MC group. When histochemically observed, C group was strongly disturbed, while MC group showed slight swelling of SMC and cell nucleus that was not remarkable though, and damage of EL and CL was minimal. AMPS and GP remained unchanged. From changes of serum lipid and observation of arterial tissue, it was suggested that Mace- has an antisclerotic effect.

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Virus-like particles (VLPs) were collected from geothermal vent water samples in the drift-way at Toyoha Mine, Hokkaido, Japan (−500 m level, 63.5°C) whose VLP and bacterial abundance was (No/ml±SD, n: 500), VLP: .60±0.29×10⁸ and bacteria: .61±0.14×10⁶. VLPs ranged in diameter from 30 to 320 nm, and the major size distribution (ca 62%) was .33. nm (n: 843). Ultrafiltration followed by CsCl density equilibrium ultracentrifugation gave purified TY-VLPs: 6.64×10¹³. Regardless of UV treatment, TY-VLP reduced the efficiency of plating to 68.6-.4% at a multiplicity of infection of ca 0. on Escherichia coli AB1157. Generalised transduction was observed on

E

. coli AB1157 with a frequency between 10^-4 and 10^-5 cells/particle using TY-VLPs without UV-treatment. The growth of generated

E

. coli transductants (TY--trans) was compared to that of an

E

. coli transductant (ST--trans) generated by Aquificales originating VLP (Chiura, 2002). The extent of the maximum growth of both transductants was ca 40% of the parental

E

. coli used as a recipient. TY--trans acquired "budding-like" particle productivity, which has been demonstrated for ST--trans. ST--trans produced five different size particles, whose DNA content ranged between 291.6 and 382.0 kb, and TY--trans produced ten different size particles between 68.5 and 190. kb, respectively.

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