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  • Sen-fang Sui, Erich Sackmann
    The Journal of Biochemistry
    1992年 111 巻 1 号 129-138
    発行日: 1992年
    公開日: 2008/11/18
    ジャーナル フリー
    In the first part of the present work the interaction of glycophorin with dimyristoylphosphatidylcholine (DMPC) is studied by freeze fracture electron microscopy, densitometry, calorimetry, and 90° static light scattering. An exothermic lipid/protein interaction energy of WP=190 kJ•mol-1 was found by application of the well known Van Laar relation for the displacement of the freezing point and the Gibbs-Duhem relationship. Secondly, the effects of Ca2+ on the lipid/protein interaction were studied. Following Ca2+ addition a remarkable decoupling of the interaction of the glycophorin head group with the bilayer surface was revealed by densitometry and gold-labeling electron microscopy. It is estimated that about 80% of lipid once disturbed by the adsorption of glycophorin head groups is decoupled after addition of Ca2+. Thirdly, the selective interaction of glycophorin with binary lipid mixtures was studied, including the mixtures of DMPC with dimyristoylphosphatidylserine (DMPS) and dilauroylphosphatidylcholine (DLPC), and the mixture of dipalmitoylphosphatidylcholine (DPPC) with DLPC.
  • 村瀬 稔, 木股 裕子, 仲西 寿男, 小澤 一弘, 赤羽 荘資, 浅川 豊, 南澤 仁志, 上條 茂徳, 小沼 博隆
    日本食品微生物学会雑誌
    2001年 18 巻 3 号 75-81
    発行日: 2001/09/30
    公開日: 2010/07/12
    ジャーナル フリー
    At low levels, enterohemorrhagic Escherichia coli should be selectively isolated by suppressing competing microflora in meat samples. In conventional methods, MacConkey II Agar with C-T Sorbitol (cefixime-tellurite, CT-SMAC) which utilizes the ability of
    E
    . coli
    O157 to ferment sorbitol, and media containing
    E.coli
    -specific chromogenic substrates, are used for detecting
    E
    . coli
    O157.
    In this study, we compared two types of
    BD
    CHROMagarTM O157 for the isolation of enterohemorrhagic
    E
    . coli
    O157 (improved
    BD
    CHROMagarTM O157 and conventional
    BD
    CHROMagarTM O157) with CT-SMAC by using the Miles-Misra method and evaluating the recovers from ground beef and human fecal samples. The results obtained are described below:
    1. In the inoculation test with three media by the Miles-Misra method, improved
    BD
    CHROMagarTM O157 inhibited the growth of all organisms except
    E
    . coli
    O157 better than the two other media and allowed easy differentiation from
    E
    . hermannii
    , which could not be distinguished on CT-SMAC.
    2. In the
    E
    . coli
    O157 detection test for ground beef artificially inoculated with
    E
    . coli
    O157 at 1 cfu/g, the detection rate of improved
    BD
    CHROMagar TM O157 was 95%, CTSMAC 75% and conventional
    BD
    CHROMagarTM O157 40%, respectively.
    4
    . In the
    E
    . coli
    O157 detection test for
    E
    . coli
    O157 positive human fecal samples, the detection rate of improved
    BD
    CHROMagarTM O157 was 54.5%, CT-SMAC 50% and conventional
    BD
    CHROMagarTM O157
    22
    .7%, respectively.
  • 石井 永, 花岡 正, 池田 仁三郎
    YAKUGAKU ZASSHI
    1976年 96 巻 10 号 1161-1168
    発行日: 1976/10/25
    公開日: 2008/05/30
    ジャーナル フリー
    In order to examine oxidation with Fremy's salt, 5-phenyl-1-naphthol (21) (mp
    93
    -
    94
    °), 5-methyl-1-naphtol (
    22
    ) (mp
    93
    -
    94
    °), 5-ethyl-1-napthol (23) (mp 78-80°), and 5-isopropyl-1-napthol (24) (mp 68-69°) were prepared from
    8
    -chloro-5-methoxy-3,
    4
    -dihydro-1 (2H)-naphthalenone (
    4
    ).
  • 1974年度と1978年度の比較
    田川 清
    食品衛生学雑誌
    1981年 22 巻 1 号 1-7_1
    発行日: 1981/02/05
    公開日: 2009/12/11
    ジャーナル フリー
    1974年度と1978年度にブロイラー鶏からサルモネラ菌および大腸菌を分離し, 薬剤耐性を比較検討した. 74年度にはサルモネラ菌の98%, 大腸菌の全菌株が供試薬剤のいずれかに耐性であり, 78年度にはサルモネラ菌の75%, 大腸菌の98.1%の菌株が耐性であった. サルモネラ菌の個々の薬剤に対する耐性菌の割合 (%) は次の如くである (かっこ内は78年度の数値). TC66 (17), SM43 (16), SA84 (59), CM13 (0), KM15 (38), APC10 (
    8
    ), CER14 (11), GM3 (3), FT90 (37), NA2 (0). 大腸菌のそれは, TC
    93
    .3 (
    85
    .3), SM69.
    8
    (65.7), SA86.
    4
    (89.
    4
    ), CM35.
    4
    (25.7), KM47.0 (52.
    8
    ), APC7.
    4
    (
    22
    .2), CER5.7 (19.0), GM3.6 (0.9), FT
    93
    .
    8
    (77.3), NA1.0 (0.7) であった. これらのことから改正法 (75年改正) による飼料添加薬剤等の規制の効果をわずかながら認めることができた. しかし大腸菌にKM, APC耐性菌の増加とRプラスミド保有菌の増加がみられたことは, 耐性菌の普遍化防止に対して困難な問題を提起している.
  • Atsushi Irie, Hideo Kubo, Motonori Hoshi
    The Journal of Biochemistry
    1990年 107 巻 4 号 578-586
    発行日: 1990年
    公開日: 2008/11/18
    ジャーナル フリー
    Glucosylceramide (G1cβ1-1Cer) was isolated from the spermatozoa of the starfish, Asterias amurensis. The long-chain bases of the glycolipid consisted of dihydroxy (d18:2, d18:3, d19:3, and d
    22
    :2), and trihydroxy (t
    22
    :1) types. Long-chain aldehydes derived from them were analyzed mainly by proton nuclear-magnetic resonance to determine the detailed structures. Two of the tri-unsaturated bases were identified as (
    4E
    ,
    8E
    ,
    10E
    )-2-amino-
    4
    ,
    8
    , 10-octadecatriene-1, 3-diol (d18:3) and (
    4E
    ,
    8E
    ,
    10E
    )-2-amino-9-methyl-
    4
    ,
    8
    , 10-octa-decatriene-1, 3-diol (d19:3), which is a novel base. Both d
    22
    :2 and t
    22
    :1 had a cis double bond at the C9 or C13 position. All fatty acids were 2-hydroxylated (C14-C25):Most of themwere saturated and unbranched. About 10% was mono-unsaturated and unbranched (
    C22
    -C25), while saturated but branched (iso- and anteiso-types) C15-C18 acids were found as mi-nor components. The main fatty acids, which summed up to more than
    93
    % of the fatty acids in the glucosylceramide, were n-14h: 0, n-15h:0, n-16h : 0, n-17h : 0, n-18h : 0, and n-24h:1.
  • L. STERU, R.D. PORSOLT
    臨床薬理
    1989年 20 巻 1 号 77-78
    発行日: 1989/03/31
    公開日: 2010/06/28
    ジャーナル フリー
  • 三宅 周, 安原 高士, 岩野 瑛二, 佐々木 俊輔, 川口 憲二, 尾上 公昭, 河野 宏
    医療
    1987年 41 巻 12 号 1028-1030
    発行日: 1987/12/20
    公開日: 2011/10/19
    ジャーナル フリー
    内視鏡的食道静脈瘤栓塞療法(以下「EIS」と略す)は, 吐血を主訴とする緊急例に対して本法がどの程度の治療的意義を持つているかについて関心がもたれている. この度, 緊急時EIS(以下「
    E
    -EIS」と略す)を施行した症例の有効性について検討したので, その成績について報告する.
    対象はEISを施行した75例中, 吐血発現後24時間以内にEISを施行した23例で, EISの方法は高瀬らに従つた.
    対象23例のうち, 1週間以上止血可能例は
    22
    例(96%)であつた. 再出血は5例(
    22
    %)にみられた. 最初の
    E
    -EIS直後悪化例はみられず, F(形態)が63%と高率に改善した. 改善維持率は, L(占拠部位)100%, F
    93
    %, RC sign(赤色所見)
    85
    %の順であつた.
    8
    例が死亡し, その死因は肝不全
    4
    例, 再吐血後肝不全3例などで, 静脈瘤出血による吐血死が激減しており,
    E
    -EISの効果が評価された.
  • Suguru TAKATSUTO, Kiyomi KOBAYASHI, Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE
    Agricultural and Biological Chemistry
    1988年 52 巻 12 号 3217-3218
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • 第1編血清蛋白との結合について
    橋本 孝夫
    CHEMOTHERAPY
    1973年 21 巻 1 号 38-44
    発行日: 1973/01/25
    公開日: 2011/03/08
    ジャーナル フリー
    The binding of penicillin G and other 29 antibiotics to the bovine plasma proteins, a part of the pharmacokinetics of antibiotics, was investigated by the ultrafiltrating technique at
    4
    °C.
    As the rates of the binding, inactivation, recovery and binding activity, following values were obtained in order : 64. 9 to 82. 7%, 11. 96 to 56.
    85
    %, 7. 77 to 25. 75% and
    8
    . 9 to 34. 11% in penicillin antibiotics; 0 to 61.
    4
    %, 0 to 10. 66%, 0. 49 to 12.
    8
    % and 14. 91 to 41. 72% in glycoside antibiotics; 91. 0 to 99. 9%, 16. 0 to 46. 19%, 44. 98 to 71. 01% and
    4
    . 33 to 21. 06% in tetracycline antibiotics; 55.
    22
    to
    94
    . 1%, 5. 37 to 57. 36%, 1. 32 to 31. 5% and 9. 66 to 40. 65% in macrolide antibiotics; and 72. 6 to 95. 68%, 14. 34 to 51.
    85
    %,
    8
    . 19 to 30. 12%, and 17. 28 to 41. 54% in chloramphenicol antibiotics, respectively.
    Optimum pH of the binding of antibiotics to the plasma proteins was in the range 5. 0 to
    8
    . 0, i.
    e
    .
    8
    . 0 in penicillin antibiotics, 5. 0 in glycoside antibiotics, 7. 0 to
    8
    . 0 in tetracycline antibiotics, 6. 0 to 7. 0 in macrolide antibiotics, and 7. 0 to
    8
    . 0 in chloramphenicol antibiotics, respectively.
  • 斉 洋之, 高津戸 秀, 池川 信夫, 田中 洋子, スミス コニー, デルカ F.ヘクター
    Chemical and Pharmaceutical Bulletin
    1984年 32 巻 10 号 3866-3872
    発行日: 1984/10/25
    公開日: 2008/03/31
    ジャーナル フリー
    Chemical synthesis of (
    22
    E
    , 24R)- and (
    22
    E
    , 24S)-1, 24-dihydroxy-
    Δ22
    -vitamin D3 has been achieved starting with the commercially available dinorcholenic acid acetate. Synthesis involved introduction of the 1-hydroxy group by a reduction of the 1, 2-epoxide generated by epoxidation of the 1,
    4
    , 6-trien-3-one. The side chain on the steroid was then constructed by means of a Wittig reaction followed by introduction of the Δ7 bond by standard methods and its protection with 1-phenyl-1, 2,
    4
    -triazoline-3, 5-dione. Subsequent reduction of the hydroxy groups in the steroid side chain followed by reduction of the Diels-Alder addition products yielded the both 24-isomers. The 5, 7-dienes were irradiated and the corresponding vitamin D compounds isolated. Nuclear magnetic resonance was used to identify individual isomers. The (
    22
    E
    , 24S)-1, 24-hydroxyvitamin D3 compound bound equally well to the chick intestinal cytosol receptor as 1, 25-dihydroxyvitamin D3, while the 24R-isomer was approximately ten times less active. In vivo, both isomers were less active than 1, 25-dihydroxyvitamin D3 ; however, the 24S-isomer was considerably more active than the 24R-isomer approaching the activity of 1, 25-dihydroxyvitamin D3.
  • Khishigjargal Batjargal, Toshihiro Tajima, Eriko Fujita-Jimbo, Takeshi Yamaguchi, Akie Nakamura, Takanori Yamagata
    Clinical Pediatric Endocrinology
    2022年 31 巻 4 号 234-241
    発行日: 2022年
    公開日: 2022/10/01
    [早期公開] 公開日: 2022/07/07
    ジャーナル オープンアクセス

    Paired box transcription factor

    8
    (
    PAX8
    ) is essential for thyroid organogenesis and development. Heterozygous pathogenic variants of
    PAX8
    typically cause congenital hypothyroidism (CH) due to thyroid hypoplasia. Additionally, pathogenic
    PAX8
    variants have been identified in patients with gland in situ (GIS). This study was conducted to analyze the in vitro functional consequences of four
    PAX8
    variants (p.D
    94
    N, p.
    E
    90del, p.V58I, and p.L186Hfs*
    22
    ) previously identified in patients with CH and GIS. The transcriptional activity of
    PAX8
    variants on the thyroglobulin (TG) promoter was assessed in a luciferase reporter assay. The levels of transcriptional activity on the TG promoter of p.
    E
    90del and p.L186Hfs*
    22
    were significantly reduced, whereas p.D
    94
    N and p.V58I showed residual activation. In addition, a dominant negative effect on the wild-type (WT) was not detected in any
    PAX8
    variant using a luciferase reporter assay. Two
    PAX8
    variants (p.
    E
    90del and p.L186Hfs*
    22
    ) may be pathogenic causes of CH with GIS.

  • Misaki Kojima, Takeya Morozumi
    Journal of Health Science
    2004年 50 巻 5 号 518-529
    発行日: 2004年
    公開日: 2004/10/01
    ジャーナル フリー
    Six full-length cDNAs encoding pig cytochrome P450 (CYP) enzymes, CYP1A1, CYP2A19, CYP2B
    22
    , CYP2C33v
    4
    , CYP2C49, and CYP2
    E
    1, were isolated and sequenced. The cDNA sequences of pig CYP1A1, CYP2A19, CYP2B
    22
    , CYP2C49, and CYP2
    E
    1 showed high similarity to human CYP1A1 (
    85
    .
    4
    %), CYP2A13 (88.6%), CYP2B6 (81.1%), CYP2C18 (
    85
    .3%), and
    CYP2E1
    (82.5%), respectively, and pig
    CYP2C33v4
    cDNA showed high similarity to rat CYP2C23 (79.2%). Reverse transcription-polymerase chain reaction (RT-PCR) assays revealed hepatic gene expression of all these pig CYP enzymes: the order of expression was
    CYP2C33v4
    and
    CYP2E1
    > CYP2C49 > CYP1A1 and CYP2A19 >
    CYP2B22
    . In the kidney, the
    CYP2C33v4
    gene was expressed at the same level as in the liver, but the CYP1A1, CYP2A19, and
    CYP2B22
    genes were expressed at lower levels than in the liver. Little renal gene expression of CYP2C49 and CYP2
    E
    1 was observed. We revealed for the first time the full-length cDNA sequences encoding pig CYP1A1 and five CYP enzymes belonging to the CYP2 gene family, thus making it possible to examine the gene expression levels of these CYP enzymes in pig tissues by RT-PCR.
  • 陳 政宇, 南雲 紳史, 梅澤 勲, 尾能 満智子, 秋田 弘幸
    天然有機化合物討論会講演要旨集
    1994年 36 巻 P-22
    発行日: 1994/09/20
    公開日: 2017/08/18
    会議録・要旨集 フリー
    One of the most efficient method to synthesize optically pure natural products is to start off with chiral synthon. We synthesized three new chiral synthons (2R,3R)-
    4
    , (
    4
    R,5S)-27, and (S)-(-)-34 on the basis of the following two concepts; (1) to utilize the highly stereoselective chemical method to establish the relative configuration, (2) to carry out the kinetic resolution of racemates by ester-cleaving enzymes (lipase) at the next step. The Zn(BH_
    4
    )_2 reduction of β-keto ester (±)-3 afforded the alcohol (±)-syn-1 in high diastereoselectivity. The saponification of the corresponding acetate (±)-
    4
    using the lipase "Amano A" proceeded enantioselectively to give acetate (2R,3R)-
    4
    (48% yield, >99%ee) and alcohol (2S,3S)-1 (51% yield,
    94
    %ee). The epoxidation of methyl sorbate followed by addition of thiophenol provided (±)-
    22
    stereospecifically. The enantioselective esterification of (±)-
    22
    using the lipase "PL266" in the presence of isopropenyl acetate gave alcohol (
    4
    R,5S)-
    22
    (49.7%, >99%ee) and acetate (
    4
    S,5R)-23 (50.2%, 98%ee). The successful conversion of C-S into C-O bond at C_
    4
    chiral center in stereo retentive manner was carried out to give (
    4
    S,5S)-21 which was converted into ketal (
    4
    R,5S)-27 via three steps. The coupling reaction of methyl
    4
    ,5-epoxy-(2
    E
    )-pentenoate 33 and m-methoxy toluene in the presence of lewis acid provided two alcohols 34 (23%) and 35 (49%). The enantioselective hydrolysis of acetate (±)-36 derived from 34 using the lipase "MY-30" gave alcohol (S)-(-)-34 (
    85
    %ee). The enzymatic esterification of this chiral compound using same lipase achieved the enrichment of optical purity. In result, we obtained (S)-(-)-36 (
    94
    %ee). These chiral compound were applicable to the syntheses of (-)-Oudemansins A, B and X, L-Acosamine, and (S)-(+)-Curcudiol.
  • 松井 吉光, 伊藤 稔明, 松田 正久
    日本物理学会講演概要集
    1997年 52.2.1 巻 22a-E-1
    発行日: 1997/09/02
    公開日: 2018/03/04
    会議録・要旨集 フリー
  • *宮嵜 伊弦
    日本行動計量学会大会抄録集
    2022年 50 巻 C22E-4
    発行日: 2022/08/28
    公開日: 2022/10/20
    会議録・要旨集 フリー
  • Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE, Kiyomi KOBAYASHI, Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 8 号 2117-2118
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • ALI TABESH, HARRY G. POULOS
    地盤工学会論文報告集
    2001年 41 巻 3 号 1-16
    発行日: 2001/06/15
    公開日: 2008/02/29
    ジャーナル フリー
    In the seismic analysis of pile foundations the soil is often assumed to be an elastic material and the pressure at the soil pile interface is not limited during the analysis. This may result in a considerable error, as the computed pressure from an elastic analysis may go well beyond the ultimate lateral pressure of real soil. In fact, significant yielding at the soil-pile interface has been observed during real earthquakes, and also in laboratory tests. The yield zone is usually near the ground surface where the effect of inertial force due to the superstructure is higher. This yielding redefines the pile response, and in general cannot be ignored. In order to examine the effects of soil yielding on the internal pile response during earthquakes an approximate analysis is described in this paper which is an extension of a static method developed by the second author (1982) for the analysis of piles subjected to lateral soil movement. This method is then used to investigate the effects of soil yielding on the internal response of piles through a comparative study in which real earthquakes are used. It is shown that for strong earthquakes and heavily loaded piles the soil yielding may considerably increase the amount of maximum pile moment developed in the pile. A marked difference in the effects of yielding on the pile moment and shear is observed and discussed.
  • ABDALLAHI.HUSEIN MALKAWI, ROBERTY. LIANG
    地盤工学会論文報告集
    1996年 36 巻 3 号 67-74
    発行日: 1996/09/15
    公開日: 2008/02/29
    ジャーナル フリー
    The interaction between a penetrating shaft and the surrounding soil media under impact loading is a frequently encountered phenomenon in geotechnical engineering. Examples of such dynamic soil/shaft interaction would include dynamic pile driving, standard penetration testing (SPT), and dynamic driven rod test. Presented in this paper is a novel approach, based on the principle of dynamic system identification, which enables identification of dynamic soil-pile interaction model parameters. The analytical transfer function, relating the output stress wave forms to the input stress wave forms in the soil/shaft system, is derived using the Laplace and Fourier Transform techniques and the one-dimensional wave propagation theories. A numerical solution algorithm based on a variation of gradient method is coded into a micro-computer based program to solve the frequency-dependent soil-pile interaction parameters (Smith model) : soil damping and soil spring stiffness. To further reduce the amount of computational effort required in the solution of the frequency-dependent dynamic soil properties, it is suggested that the solution be obtained for the first-mode natural frequency of the measured stress waves. The results obtained from this simplified solution algorithm, when compared with both numerical simulations and controlled laboratory tests, are reasonably acceptable. The proposed parameter identification technique offers a viable alternative data interpretation procedure for deducing pertinent Smith model parameters used frequently in pile driving analysis.
  • Ying Jiao, Teruhiko Yoshihara, Akitami Ichihara
    Bioscience, Biotechnology, and Biochemistry
    1995年 59 巻 6 号 1032-1035
    発行日: 1995/06/23
    公開日: 2008/02/08
    ジャーナル フリー
    The syntheses of (S)-13-hydroxy-(2
    E
    ,
    4
    E
    ,
    8
    E
    )-tetradecatrienoic acid (1) and (2
    E
    ,
    4
    E
    ,
    8
    Z-tetradecatrienoic acid (2) were carried out by using the Wittig reaction as the key step. The asymmetric center at C-13 and the double bond between C-
    8
    and C-9 for natural compound 1 were reconfirmed as being of (S) configuration and
    E
    , respectively. The relationship between the structure of the unsaturated hydroxy fatty acids and their inhibitory effect on the growth of lettuce was investigated.
  • 橋本 聡一, 淡田 修久, 李 龍彦, 中元 賢武, 中村 憲二, 井内 敬二, 森 隆, 長岡 豊, 沢村 献児, 大川 晋一, 深井 保, 小谷口 浩人, 西原 哲夫, 萩原 文二
    人工臓器
    1982年 11 巻 1 号 234-237
    発行日: 1982/02/15
    公開日: 2011/10/07
    ジャーナル フリー
    Transcutaneous carbon dioxide tension (tcPCO2) were continuously measured during open heart surgery for fifteen patients aging from 5 to 58.
    The Hagihara transcutaneous CO2 electrode was used. This modified Severinghaus blood gas electrode consists of a PH-sensitive glass electrode and a silver-silver chloride reference electrode covered by a hydrophobic CO2 permeable membrane from which they are separated by a sodium bicarbonate electrolyte solution. The heating unit is also contained in this sensor-unit to dilate skin capillaries. Sensor temperature was maintained at 44°C throughout the study.
    This sensor was attached at the thigh of the patient. During open heart surgeries, 117 arterial blood samples were taken for the determination of PaCO2. The regression curve between tcPCO2 and PaCO2 was
    tcPCO2=1.11×PaCO2+9.5.
    Using this formula, corrected tcPCO2 was calculated. During operation, at every thirty minutes the tcPCO2 and PaCO2 was determined and during
    E
    . C. C., at every fifteen minutes, the determination was made.
    The mean value of corrected tcPCO2 showed no statistical difference from that of PaCO2 throughout the study. Before
    E
    . C. C., the corrected tcPCO2 ranged between 26.
    8
    and 34.5 and the PaCO2 between 27.5 and 34.2 During
    E
    . C. C., the corrected tcPCO2 ranged between 18.6 and
    22
    .9, PaCO2 between 17.6 and 21.5. After
    E
    . C. C., the corrected tcPCO2 ranged between
    22
    .2 and 37.
    4
    , PaCO2 between 24.
    8
    and 33.
    8
    mmHg respectively.
    This value of tcPCO2 with this machine proved to have a good coefficient with that of PaCO2 (r=0.
    93
    ).
    In conclusion, this transcutaneous CO2 electrode is very useful for the monitoring of PaCO2 during open heart surgery.
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