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  • Yosizo YAMAMOTO
    Nippon Sugaku-Buturigakkwai Kizi Dai 3 Ki
    1940年 22 巻 12 号 1048-1052
    発行日: 1940年
    公開日: 2009/06/09
    ジャーナル フリー
    The numerical values of the intervals betrween optieal levels are competed for the configurations
    1s22s22p83s23p83
    *
    94s
    ,
    5
    s, 6s and
    7
    s of Cu+, according to the general expression of energy-levels derived in the previous paper The self-consistent field radial functions computed by Hartree adn Hartree are used for1s, 2s, 2p, 3s, 3p and 3
    d
    . Those of 4s,
    5
    s,
    e
    .s and are ealenlated from Hartree Hartree's core-functions by the numerical integrations. The calculated results are shown in Table I.The agreement with experiment is satisfactory
  • Joey
    B
    . Tanney, Leonard J. Hutchison
    Mycoscience
    2012年 53 巻 1 号 31-35
    発行日: 2012年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–1,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

  • 藤原 東雄, 上 俊二
    土質工学会論文報告集
    1990年 30 巻 1 号 76-86
    発行日: 1990/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    The behavior of the soft clay grounds subjected to repeated loading is different from that subjected to sustained loading. The difference in settlement between these two loading patterns should be due to secondary compression over a long period of time. Consequently, soft clay grounds subjected to repeated loading tend to be more compressible than those subjected to sustained loading. Therefore, it is necessary for engineers to predict post-construction settlements under repeated loading. In this respect, the preloading is considered to be promising as a countermeasure to reduce the settlement of clay under repeated loading as well as under sustained loading. The effect of preloading on post-construction consolidation settlement of soft clay subjected to repeated loading after removal of a part of preload is investigated in the present paper. It has become clear that the settlement of a clay sample after preconsolidation is mainly affected by the amount of preload, the degree of consolidation due to the preload, the amount of permanent load and the amount of repeated load after removal of preload. The calculated settlement versus time relations using a method to estimate the amount of consolidation settlement of soft clay grounds subjected to repeated loading after removal of preloading were compared with the observed degree of consolidation as parameters of the intensities of preload and repeated load.
  • Yoshio UEDA
    Journal of geomagnetism and geoelectricity
    1988年 40 巻 12 号 1481-1501
    発行日: 1988年
    公開日: 2010/09/14
    ジャーナル フリー
    Sea-bottom topography, magnetic and gravity anomaly maps were compiled for two seamounts, Smt-948 (23.
    7
    °N, 151.
    9
    °
    E
    ) and Smt-1100 (
    22
    .
    8
    °N, 153.4°
    E
    ) in the vicinity of Minami-Tori Sima (Marcus) Island based on survey data observed by the S/V Takuyo of the Hydrographic Department of Japan from July to August, 1985. These flat-topped seamounts are located in the Jurassic magnetic quiet zone on crust older than 150Ma. On Smt-1100, sea bottom photographs were taken with a deep-sea camera and three dredge hauls were made. Three-dimensional magnetic analyses of Smt-948 gave a paleomagnetic pole position of 57.
    5
    °N, 329.
    5
    °
    E
    with the goodness-of-fit ratio 2.
    83
    . The pole position lies in the Pacific apparent polar wander path between the Late Jurassic (150Ma) and the Late Cretaceous (65-
    94
    Ma). This result implies that Smt-948 was generated in the middle Cretaceous, a significant period after the formation of the Jurassic sea-floor. Two-dimensional modeling of magnetic and gravity anomalies across these seamounts reveal a caldera-like basement structure, suggesting a constructional origin of the flat top of the guyot. A sequence of normal and reversed magnetic polarity layers is estimated from magnetic modeling on Smt-1100.
  • 井合 進, 森田 年一, 亀岡 知弘, 松永 康男, 安彦 和之
    土質工学会論文報告集
    1995年 35 巻 1 号 115-131
    発行日: 1995/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    In the 1993 Kushiro-oki Earthquake of Richter magnitude
    7
    .
    8
    , simultaneous recording of earthquake motions was successfully made at the ground surface and at a depth of 77 meters in a dense saturated sand deposit. The peak horizontal acceleration was 0.47 g on the ground surface and 0.21 g at a depth of 77 meters. The acceleration record at the ground surface showed a distinctive ground response, which consisted of a cyclic motion having a period of about 1.
    5
    seconds overlain by a spike at each peak of the motion. In order to study the mechanism of this peculiar ground response, effective stress analysis was conducted on the dense saturated sand deposit. The model used for this study was a strain space multiple mechanism model, which takes into account the effect of principal stress axis rotation. The recorded earthquake motion at a depth of 77 meters was used as the input earthquake motion for the analysis. Sampling after in-situ freezing was done in order to evaluate the properties of the sand. The results of the analysis indicated that the observed ground response was due to the effect of dilatancy of sand, which plays a significant role in the response of the dense saturated sand deposits during strong earthquake motions.
  • 國生 剛治
    地盤工学会論文報告集
    2000年 40 巻 5 号 99-111
    発行日: 2000/10/15
    公開日: 2008/02/29
    ジャーナル フリー
    In view of the significant role of the water film effect in flow failure for a liquefied sandy deposit, the mechanism of water film generation is numerically studied based on a 1-dimensional model test. The process of water film growth and decay can be simulated to a certain extent by a simple consolidation analysis, which indicates that only a small difference in permeability in layered sand is enough for a water film to develop. A 1 G shaking table test for a two-dimensional slope model with an arc of silt within a saturated sand is then addressed to discuss the dilatancy effect exerted in sheared sand during flow failure. It is possible that, once the water film is formed, the transmission of shear stress through it is interrupted, leaving the sand below free from the dilatancy ; this eventually allows the water film to stay without being absorbed during flow failure. The result of another shaking table test for a trapezoidal slope with horizontal silt seams indicate that water films beneath the seams enable the soil mass above them to laterally flow along water films very gently inclined even after shaking. If a silt seam breaks due to excessive pressure in the water film, it triggers re-liquefaction in the upper sand and leads to further instability.
  • T BAKER, C. RANGACHARYULU
    日本物理学会講演概要集
    1999年 54.2.1 巻 23pSM-6
    発行日: 1999/09/13
    公開日: 2018/03/04
    会議録・要旨集 フリー
  • 金谷 守, 河井 正, 栃木 均
    地盤工学会論文報告集
    2001年 41 巻 6 号 79-96
    発行日: 2001/12/15
    公開日: 2008/02/29
    ジャーナル フリー
    In this paper, centrifuge shaking table tests were conducted in order to understand the performance of seawalls during a seismic event. The model tests showed that the displacement of the caisson was much affected by the seaward shear deformation of the sand seabed beneath it during shaking. It was also confirmed that an armored embankment played an important role in the displacement of the caisson during shaking. Based on these test results, a two-dimensional DEM-FEM coupled analysis method was newly developed to numerically predict the deformation of seawalls covered with armored embankments during earthquakes. The movements of the armor units were calculated by DE analysis and deformations of the caisson, rubble mound, sand seabed and backfill were calculated by FE analysis considering the non-linearity of the soil materials based on the effective stress. Dynamic interaction was taken into account by delivering the nodal displacements of the finite elements or the nodal forces converted from the contact forces through the imaginary distinct elements defined at the boundaries between the DE and FE domain. The applicability of this method to the prediction of the deformation of seawalls was verified through numerical simulations of the centrifuge model test.
  • 本城 勇介, 岩本 悟志, 杉本 三千雄, 鬼丸 貞友, 吉澤 睦博
    地盤工学会論文報告集
    1998年 38 巻 1 号 131-143
    発行日: 1998/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    Seismic records obtained from a seismometer array located in downtown Tokyo Japan for about ten years were inversely analyzed to estimate the dynamic soil parameters. Due to the illposed nature of the problem, the simple and often used "least square method" does not properly estimate the parameters. The Extended Bayesian Method combined with the Akaike Bayesian Information Criterion was introduced to overcome this difficulty. The results obtained were compared with dynamic triaxial test results obtained at the time of the seismometer installation. The shear moduli agree quite well with the estimated results, however the damping ratios estimated are slightly higher than the ones obtained in the laboratory.
  • 澁谷 啓, 田中 洋行
    地盤工学会論文報告集
    1996年 36 巻 4 号 45-55
    発行日: 1996/12/15
    公開日: 2008/02/29
    ジャーナル フリー
    An empirical equation in use for estimating the pseudo-elastic shear modulus, Gf, of subsoil, associated with shear strains less than 0.001% is proposed in this paper. In a series of in-situ seismic cone tests performed nationwide, the profiles of both Gf and the in-situ void ratio,
    e0
    , with depth were successfully characterised at five sites, each comprising a soft clay layer deposited in the Holocene era. The database which comprised the original data from the field and laboratory tests, coupled with similar information on well-documented Holocene clay deposits in Europe, was statistically analyzed in attempts to determine a generalised relationship with which Gf of soft clay may be reasonably estimated only from routinely available borehole data; that is
    e0
    and the current geostatic effective overburden pressure, σ'v. An empirical relationship, Gf=
    5
    , 000
    e0
    -
    1.5
    √(σ'v) (kPa), was derived from the statistical analysis applied to data from seven different clays worldwide, for which
    e0
    extended over a range between 1 and
    5
    , and the overconsolidation ratio ranged roughly between 1 and 2. The applicability of the proposed relationship was evaluated for two case records, each in which the clay exhibited unusual behavior; i.
    e
    ., the undrained shear strength remained more or less constant with depth due to the existence of artesian pressure at one site, and, at the other, Gf decreased, whereas
    e0
    increased, with depth. It was demonstrated that even in these clay deposits exhibiting exceptional profiles, the proposed relationship was capable of predicting Gf with a reasonable accuracy by determining the profiles of
    e0
    and σ'v with depth. In addition, the prediction when compared to Gmax from carefully performed laboratory cyclic tests, yielded a better estimate of Gf from the in-situ seismic survey. Despite the fact that the empirical relationship was initially designated to estimate Gf of soft clays, it may be equally applicable to sandy deposits. This was verified by comparing it to similar, and well-established, relationships developed for sands. A case record as such is also described for a loose sand deposit at Higashi-Ohgishima in Tokyo Bay which was placed in 1960's by land reclamation.
  • A.M. BRITTO, C. SAVVIDOU, M.J. GUNN, J.R. BOOKER
    土質工学会論文報告集
    1992年 32 巻 1 号 13-25
    発行日: 1992/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    One method currently being considered for the disposal of high level radio-active waste is burial in the seabed. When a hot object is buried in soil, the temperature of the soil adjacent to the object is raised and there is transient heat flow away from the object. The rise in the temperature of the soil causes excess pore pressures to be generated and transient pore water diffusion also takes place. A finite element formulation of the coupled heat flow and consolidation problem is presented. The computer implementation of this formulation is found to compare favourably with analytical solutions based on the same physical principles.
  • 琴尾 幸徳, 石川 成実, 田辺 順子, 御園 生尭久
    日本化学会誌(化学と工業化学)
    1980年 1980 巻 9 号 1391-1396
    発行日: 1980/09/10
    公開日: 2011/05/30
    ジャーナル フリー
    Nアルキル3,4:
    9
    ,10-ペリレンテトラカルボン酸モノアンヒドリド=モノイミド[4a~
    e
    ]と芳香族アミン(アニリン,p-トルイジン,p-アニシジン,3,
    5
    -キシリジン,4-アミノナゾベンゼン,およびo-フェニレンジアミン)を縮合して非対称型3,4:
    9
    ,10-ペリレンビス(ジカルボキシミド)誘導体-N-アルキル-N'-アリール-3,4:
    9
    ,10-ペリレンビス(ジカルボキシミド)(〔
    5
    a~
    e
    〕,〔6a~
    e
    〕,〔
    7
    a~
    e
    〕,〔
    8
    a~
    e
    〕,〔
    9
    a~
    e
    〕,および〔10a~
    b
    〕)を合成した. これらの各誘導体はすべて赤色系の色相を示し, 顔料試験の結果N-ブチル-N'-アリール-3,4:
    9
    ,10-ペリレンビス(ジカルボキシミド)(たとえば〔
    5
    e
    〕や〔6
    e
    〕)がとくにすぐれた耐光性を示した.
  • 安原 一哉, 村上 哲, 豊田 紀孝 /, ADRIAN F.L. HYDE
    地盤工学会論文報告集
    2001年 41 巻 6 号 25-36
    発行日: 2001/12/15
    公開日: 2008/02/29
    ジャーナル フリー
    Based on the methods previously presented by the authors (Yasuhara et al., 1992, 1994, 1996) for predicting the degradation in strength and stiffness of soft clays in the course of cyclic loading, a methodology has been developed to estimate the cyclic loading-induced settlements. The method also includes not only immediate settlements but also post-cyclic long-term settlements due to dissipation of cyclically induced excess pore pressures in soft soils. The simplified formulae included in the proposed methodology are given as functions of the amplitude of cyclic-induced excess pore pressure normalized by the confining pressure, u/p'c, plasticity index Ip and factor of safety against bearing capacity failure, Fs. The calculations of cyclic-induced settlements were conducted for soft soil deposits with different index and geotechnical properties. The results calculated using the proposed methodology are presented in the form of a design chart to give the settlement versus normalized excess pore pressure ratio relations including the effects of the plasticity index and safety factor for bearing capacity. An example of the calculated results using the proposed procedure for the earthquake-induced settlements of embankments founded on soft clay, is presented to demonstrate the practicality of the method for design at fields.
  • 安田 進, 伯野 元彦, 永瀬 英生, 小林 正二郎, 吉田 剛
    土質工学会論文報告集
    1994年 34 巻 1 号 65-76
    発行日: 1994/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    Shaking table tests were performed to clarify the settlement in liquefied loose sand of a new foundation incorporating top-shaped concrete blocks. In the tests, blocks of half scale were installed as they are in practice, by using a crusher-run, a lattice of iron rods and iron bars. Shaking table tests were also conducted on foundations with two differently shaped concrete blocks to investigate the effectiveness of the three foundations in reducing settlement. One was a T-shaped concrete block, or a disk-shaped concrete plate with a leg. The other was a conical concrete block without a leg. The results of shaking table tests showed that the foundation with the top-shaped concrete blocks was most effective in reducing the settlement among the three types of foundation. FEM elastic analyses were performed to compute the stress distributions in the models and in-situ ground when the top-shaped concrete blocks were applied and to confirm whether liquefaction resistance of the sand layer below the foundation is large. Moreover, the factor of safety against liquefaction, FL was evaluated in in-situ model ground, in order to compare with sensitivities against liquefaction in the ground without countermeasures and with top-shaped concrete blocks. For the liquefaction analyses, shear stress in the ground during an earthquake was estimated by dynamic analyses using the SHAKE computer program.
  • ANESTIS S. AVRAMIDIS, SURENDRA K. SAXENA
    土質工学会論文報告集
    1990年 30 巻 3 号 53-68
    発行日: 1990/09/15
    公開日: 2008/02/29
    ジャーナル フリー
    The original Drnevich Resonant Column apparatus has been designed for testing cylindrical soil specimen with maximum longitudinal and torsional stiffnesses corresponding to approximate resonant frequencies 285 Hz and 318 Hz respectively. This apparatus has been modified successfully and its stiffness has been increased to over threefold in the longitudinal vibratory mode and at least twofold in the torsional vibratory mode. Monterey No. O sand samples prepared at approximate relative densities 43% and 60% were tested with the original and the modified apparatus in the range of effective confining pressures from 49 kPa to 588 kPa. Measurement of dynamic moduli values measured with the original apparatus were up to 23% higher, and damping values up to five times higher than those measured by the modified apparatus.
  • ―特にアポ蛋白Eの変化について―
    本間 康彦, 三神 美和, 佐藤 美智子, 石原 仁一, 吉川 広, 木下 栄治, 田川 隆介, 星合 充基, 古屋 秀夫, 井出 満, 田辺 晃久, 玉地 寛光, 兼本 成斌, 友田 春夫, 中谷 矩章, 五島 雄一郎
    動脈硬化
    1984年 12 巻 3 号 599-603
    発行日: 1984/08/01
    公開日: 2011/09/21
    ジャーナル フリー
    Seven hundred fifty mg of cholesterol were fed daily to 32 patients of the ischemic heart disease (IHD) for 2 weeks. Cholesterol amounts in VLDL, LDL, HDL, HDL2 and HDL3 were estimated on the 0th,
    7
    th and 14th days of cholesterol load. Plasma apoprotein A-I, A-II,
    B
    , C-II,
    E
    levels were also measured in 16 from 32 IHD subjects. Apoprotein levels were estimated by a method of single radial immunodiffusion (SRID). Before cholesterol administration, cholesterol amounts in VLDL, LDL, HDL, HDL2 and HDL3 were 15.
    9
    ±
    7
    .
    5
    mg/dl (mean±SD), 150.
    9
    ±58.1mg/dl 43.
    8
    ±
    9
    .6mg/dl, 16.4±
    5
    .
    8
    mg/dl and 24.
    9
    ±6.3mg/dl respectively. Plasma apoprotein A-I, A-II,
    B
    , C-II and
    E
    levels were 81.
    7
    ±
    22
    .0 mg/dl, 15.1±
    5
    .4mg/dl, 93.
    7
    ±36.0mg/dl, 3.
    83
    ±1.00mg/dl and 4.29±1.36mg/dl respectively. After 2 weeks' cholesterol feeding, all plasma lipoprotein cholesterol and apoprotein levels did not change significantly. Correlation coefficients between plasma apoprotein and lipoprotein cholesterol levels were calculated. Apoprotein A-I and A-II correlated with HDL-C (r=0.348), HDL2-C (r=0.612) and HDL3 (r=0.569). Apoprotein
    B
    correlated with total cholesterol (TC) (r=0.610), VLDL-C (r=0.341), LDL-C (r=0.726), HDL-C (r=0.432) and HDL2-C (r=0.465). Apoprotein C-II correlated with TC (r=0.765), TG (r=0.679), VLDL-C (r=0.651), LDL-C (r=0.461) and HDL2-C (r=0.326). Apoprotein
    E
    correlated with TC (r=0.580), TG (r=0.575) and VLDL-C (r=0.666). Interapoprotein's correlations were also calculated. Apoprotein A-I correlated with apoprotein A-II (r=0.468). Apoprotein
    B
    correlated with apoprotein C-II (r=0.393). Apoprotein C-II correlated with apoprotein
    B
    (r=0.393) and
    E
    (r=0.549). Apoprotein
    E
    only correlated with apoprotein C-II (r=0.549).
  • 斎藤 徹, 井上 勲, 藤井 澄三
    Chemical and Pharmaceutical Bulletin
    1990年 38 巻 6 号 1536-1547
    発行日: 1990/06/25
    公開日: 2008/03/31
    ジャーナル フリー
    A full account is given of the chemical behavior observed for
    7
    ,
    9
    -dialkyladeninium salts (16). On treatment with boiling 1N aqueous NaOH for 60min, 16a,
    b
    ,
    d
    ,
    e
    (X=I), 16c (X=Br), and 16f (X=ClO4) rearranged to isomeric N6,
    7
    -dialkyladenines (21a-f) in 50-91% yields. Treatment of the salts with 0.
    5
    N aqueous Na2CO3 at room temperature for 30-90min or with Amberlite CG-400 (OH-) in H2O at room temperature gave the ring-opened derivatives
    22
    a-f (in the trans-formamide form) in 56-
    83
    % yields, and rate constants for the ring-opening reactions of 16a,
    b
    ,
    d
    -g (X=ClO4) and 16c (X=Br) leading to
    22
    a-g were determined in H2O at pH
    9
    .84 and ionic strength 0.50 at 25°C. Cyclization of
    22
    a with NaH in AcNMe2 at room temperature or with boiling 1N aqueous NaOH produced 21a in 84% or 72% yield, respectively.In solution, the trans-formamides
    22
    seemed to transform slowly into the cis-formamides 23, attaining equilibria. The existence of such an equilibrium in
    D2O
    or Me2SO-
    d6
    at 25°C or in H2O at pH
    9
    .84 and ionic strength 0.50 at 25°C was kinetically confirmed in the case of
    22
    a, and the mechanism of the rearrangement of 16 to 21 through
    22
    is discussed on the basis of the above kinetic results and Deslongchamps' theory of stereoelectronic control. On treatment with NaBH4 in MeOH at room temperature, 16a (X=I) furnished the
    7
    ,
    8
    -dihydro derivative 28 (84% yield), which slowly decomposed in H2O at 60°C to give
    22
    a in 49% yield.The
    7
    ,
    9
    -dialkyladeninium salts (16) were found to be obtainable from N'-alkoxy-1-alkyl-
    5
    -formamidoimidazole-4-carboxamidines (
    9
    ) through an alternative synthetic route : Alkylations of
    9
    with alkyl halides in HCONMe2 in the absence of base, followed by hydrogenolysis of the N'-alkoxy group and cyclizatio (or vice versa) produced 16 in acceptable yields. In order to interpret the proton nuclear magnetic resonance spectrum of
    22
    a, the 2-deuterated species 26 was also synthesized from 24 via 25 and 27.
  • 澁谷 啓, 三田地 利之
    土質工学会論文報告集
    1994年 34 巻 4 号 67-77
    発行日: 1994/12/15
    公開日: 2008/02/29
    ジャーナル フリー
    In this paper, consideration is given as to how to characterize depth-variation for the small-strain shear modulus of natural clay sedimentation, in a state of normal consolidation. A case study was carried out for a relatively uniform clay layer deposited in the Holocene era. Initially, the effects of both strain and in-situ stress levels on secant shear modulus were carefully examined in cyclic torsion shear tests using undisturbed samples, which were recovered at different depths in a test borehole. The range of shear strain examined was between 0.001% and 1%. Similar examination was made for a silty clay using reconstituted samples that were isotropically consolidated at different stress levels. On the basis of the results of these laboratory tests, together with the shear modulus from an in-situ seismic survey, the small-strain shear modulus was formulated in terms of the stress and strain levels, and linked also to undrained shear strength. Interactions of the small strain stiffness between in-situ and laboratory are discussed in depth with an attention paid to the existing aging effect in the original subsurface condition.
  • 大澤 康次, 幡野 恵, 西宮 一尋, 岡崎 彬, 太田 真一, 宇田 文昭, 柳田 由紀, 檜垣 洋子, 吉田 知江里
    薬物動態
    1988年 3 巻 4 号 441-446
    発行日: 1988年
    公開日: 2007/03/29
    ジャーナル フリー
    ラットに3H-CU-
    83
    (S)を25μg/kgで静脈内あるいは経口投与し,血液中濃度および尿糞中排泄を検討した.
    静脈内投与後の血液中濃度推移は投与後
    5
    分より上昇し,投与後45分に25.74ng eq./mlのCmaxを示し,それ以後t1/2 3.05時間とt1/2 33.09時間の二相で減少した.投与後72時間までのAUCは135.42ng eq.·hr/mlであった.
    経口投与では,投与後3時間でCmax 4.10ng eq./mlに達し,以後t1/2α 4.46時間とt1/2β 26.
    83
    時間の二相で減少した.投与後72時間までのAUCは48.62ng eq.·hr/mlであった.
    静脈内投与と経口投与のいずれの場合も,尿および糞中への放射能の排泄は,投与後48時間でほぼ終了した.静脈内投与では,投与後72時間までに投与量の30.52%が尿中に,60.42%が糞中に排泄された.経口投与では,同じく72時間までに40.34%が尿中に,69.24%が糞中に排泄された.
  • 百目鬼 郁男, 中原 達夫, 山内 亮
    家畜繁殖研究會誌
    1974年 20 巻 2 号 76-80
    発行日: 1974/08/30
    公開日: 2008/05/15
    ジャーナル フリー
    性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
    Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-2AおよびType203で測定した結果,励起光538nm,螢光552.
    5
    nmであった。この螢光特性は
    E1
    ,
    E2
    および
    E3
    にそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•
    5
    ±
    22
    5nm
    を読み,ALLENの補正を行なった。この条件において
    E1
    ,
    E2
    および
    E3
    -methyletherの最少検出量は1ngであった。回収率補正の目的で加えた6,
    7
    -3H-
    E2
    -17βの全過程における回収率は平均60.3±11.
    7
    %であった。正常性周期を示す黒毛和種2頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共
    E1
    ,
    E2
    の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,
    E2
    E1
    にくらべ全般に高値であった。また
    E3
    は検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.3および
    99.8ng
    /l;
    E15.9
    および16.0ng/l,
    E229.4
    および
    83.8ng
    /l)を形成し,排卵後は急激に減少して最低値(3.
    8
    ~
    5.3ng
    /l;
    E11.6
    および
    1.9ng
    /l,
    E22.2
    および3.4ng/l)を示した。黄体期の最高値(10.1および27.0ng/l;
    E12.4
    および3.4ng/l,
    E27.7
    および23.6ng/l)は排卵後6~
    8
    日に認めた。すなわちestrogenの血中濃度は性周期の間に2つのピークを形成することを認めた。
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