A monoclonal antibody, established from mice injected with dolphin peripheral blood lymphocytes (PBLs) was characterized. In addition to its reactivity against .% of dolphin PBLs, reacted with 33.% of bovine PBLs of which % were CD ⁺, 11.% were -. recognized a 34 kD protein on the surface of dolphin and bovine PBLs. Analysis of the protein's N-terminal amino acid sequence indicated that recognizes bovine major histocompatibility complex (MHC) class II antigens. These results suggested that recognized MHC class II antigens on bovine PBLs. As we have already produced an anti-dolphin MHC class I monoclonal antibody, analysis of immune system using these monoclonal antibodies will advance our understanding of the evolution of the mammalian immune system.

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Repetitive liquefactions in angular and subangular gravels from an alluvial fan, in the case of two earthquakes with accelerations lower than that of a former which had caused liquefaction some months before, are described. The liquefaction took place from the surface, down to a depth of approximately 10 m. Water laden with sediments spouted out for some tens of minutes after the strong motion ceased. Knowledge of the grain size distribution of the sediments inside the body of the alluvial fan roughly defines the areas of the fan prone to future liquefaction. Non-repetitive liquefaction in overconsolidated fine fluvio-lacustrine sand of glacial origin at the unusual depth of 14 m to 16 m is also described. From the observation of sand lifted up for 10 m inside a large diameter well, and of that which spouted out from several microvents, it was estimated that extra-pore pressure was close to lithostatic conditions. Nevertheless, modeling of intrinsic pore pressure buildup on the DESRA2 programme gives lower values than the experimental one. Geotechnical characteristics of the 12 m-thick sandy stratum indicate that the upper part is unusually soft, either due to water circulation or past repetitive liquefactions which prevented reconsolidation. It is suggested that excess pore pressure generated by the earthquake was also due to redistribution of pressure from adjacent sediments.

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We compared three groups of pregnant women: placebo with normotensive women, group A which included preeclamptics, and group which comprised preeclamptics who were supplemented their diets with vitamins and . MDA increased from . ± 2. (placebo) to .48 ± .2 (A) and .02 ± . nmol/gHb (). NO concentrations were enhanced from 19.3 ± .2 (P) to 23. ± . (A) and 24. ± . μmol/L (). GSH contents were decreased from 10.42 ± 2.81 (P) to .02 ± 2.92 (A) and .39 ± .02 μmol/g Hb (), whereas GSSG concentrations increased from 0.98 ± 0.28 (P) to .24 ± 0.29 (A) and .08 ± 0.12 μmol/g Hb (). SOD activity decreased 23% in A and 14% in ; GRx decreased 27% in A and .% in ; GPx decreased 12% in A and .% in . Catalase activity, however, increased 27% in A and 29% in as compared to control. Thus, we conclude that the use of vitamins and should be considered for the control of certain important biochemical indices during the development of preeclampsia; however, further studies are needed to develop methods for the prevention of preeclampsia in women at high risk.

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The phase diagrams of the Ni-Mo- and Ni-W- ternary systems in the region of less than 50mol% were constructed by thermodynamic calculation, based on the data obtained by thermodynamic measurement of the related materials. We found three ternary eutectic points and three or two ternary peritecto-eutectic points as follows:
:L (1365K, 71.mol%Ni-.0mol%Mo-.mol%)=(Ni)++
:L (1355K, 62.mol%Ni-2.mol%Mo-30.mol%)=++
:L (1445K, 42.0mol%Ni-30.mol%Mo-10.3mol%)=(Ni)+NiMo+
:L (1812K, 34.mol%Ni-42.3mol%Mo-.mol%)+MoB=+
P₂:L (1633K, 42.3mol%Ni-40.mol%Mo-17.3mol%)+Mo=+
P₃:L (1812K, 53.mol%Ni-33.7mol%Mo-12.mol%)+Mo=NiMo+
:L (1622K, 51.0mol%Ni-31.mol%W-17.mol%)=(Ni)+W+
:L (1260K, 71.0mol%Ni-7.0mol%W-.0mol%)=(Ni)++
:L (1291K, 65.mol%Ni-.mol%W-29.mol%)=++
:L (2115K, 23.mol%Ni-43.mol%W-33.mol%)+WB=+
P₂:L (1657K, 48.mol%Ni-33.mol%W-18.0mol%)+=W+
The calculated phase diagrams are expected to be useful for the development of new Ni-based heat-, corrosion- or wear-resistance alloys.

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Five new marine _<29> sterols (2-) were isolated from the soft coral Sinular ia mayi of the Ishigaki Island, Okinawa. The Spectrosocpic data showed that they are the polyhydroxy derivative of sarcosterol (), a marine sterol having a unique 23-methyl and a 17(20)-double bond. Sterol 2 was the 25-monohydroxy derivative of . The NOE experiment of its 25, 26-didehydro derivative indicated that the stereochemistry at -23 and 24 was identical with that of peridinosterol, whose absolute configuration has previously been established. Sterol 3 was the 25,26-dihydroxy derivative of . It was converted to 2 by reductive cleavage of the -26 tosyloxy bond by LAH. Sterols and were shown, by spectroscopic analyses, to be , 25-dihydroxy and ,23,25-trihydroxy derivatives of . The stereochemistry in the side chain was derived from interpretation of the coupling pattern and the results obtained by NOE experiments. On storage sterol was found to be converted, by dehydration, to sterol having a tetrahydrofuran ring on the side chain Two new cembranoids sinulariol (7) and mayolide (), simultaenously isolated from S. mayi, were correlated to the known compounds sinulariol A () and mayolide (10) by dehydration. The pyridine-induced deshielding effects, observed in the ^H NMR spectra of and 10-12, indicated that the absolute configuration at - of to be (R) and that of 10-12 to be (S). A new polyhydroxygorgostane (15) was obtained from the Andaman Sea soft coral Lobophytum strictum. The structure was derived, by spectroscopic data. to be β-hydroxy--deoxy derivative of the known compound andamansterol.

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Agaricus blazei is an important fungus for producing bioactive compounds. There are some reports of polysaccharides and steroid derivatives from the fruiting bodies of A. blazei. However, the chemical examination of the secondary metabolites of the cultured mycelia of this species has not been reported. Eight compounds, an unprecedented skeleton have been isolated from the cultured mycelia of A. blazei. The structures of the novel compounds each named blazeispirols A (), (2), (3), D (), () X(), Y (7) and Z () were confirmed by extensive D and 2D NMR spectral data and X-ray analysis. Blazeispirols A (), (2), (3), D () and () were des-A-ergostane type compounds having spiroacetal structure as a side chain. Blazeispirols X () and Y (7) were determined to be (20S, S, 23R, 24S)- (10→) abeo-14β, :, 25-diepoxyergosta-,7,,11-tetraene-3α,23-diol and (20S, S, 23R, 24S)-14β,:,25-diepoxy-,23-dihydroxyergosta-,7,11-triene-3,-dione by comparison of extensive D and 2D NMR spectral data with that of blazeispirol A. The biosynthesis of blazeispirol A was investigated by feeding ^<13>-labeled acetates and methionine to the growing cultures of A. blazei. The labeling patterns of derived from singly and doubly ^<13>-labeled acetates were consistent with that of ergosterol reported previously except for the A-ring and then ^<14>-labeled ergosterol was incorporated into blazeispirol A. Taking the structures of blazeispirols Z and D into consideration, it can be assumed that blazeispirol A is biosynthesized from ergosterol by the cleavage of -, - and -, -10 bonds on retro aldol condensation and Michael reaction via such as intermediate A as shown in Fig. . A large number of ergostane-type steroids have been isolated from many fungi. However, blazeispirol A is the first example of a naturally occurring des-A-ergostane-type steroid including a spiroacetal structure moiety as a side chain.

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This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

. coli isolates were detected in 95 (.2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: bla_CTX-M-15 (62), blaTEM-

1b

(42), bla_CMY-2 (

22

), bla_CTX-M-3 (16), blaCTX-M-

1

(15), blaOXA-

1

(

9

) and bla_SHV-12 (3) alone or in combination. The most frequently encountered phylogenetic group was group

A₁

(35.

8

%), followed by group D₂ (

22

.

1

%),

B

1

(15.

8

%),

D₁

(

9

.

5

%), A₀ (7.

4

%),

B2₂

(

5

.3%) and

B2₃

(

4

.2%), respectively. PMQR genes, aac(

6

’)-Ib-cr,

qnrS1

and qnrB10 were detected in 25.3, 10.

5

and

1

.

1

% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.0% for tobramycin to 56.

8

% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE, f17a-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing

E

. coli clone O25

b

:ST131/

B

2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing

E

. coli isolates among dogs in Turkey.

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A sample of [³H] tobrarnycin (, 000 Ci/Mole) has been synthetized and incubated with
the bacterial ribosome and its subunits. The results obtained show that this antibiotic has
two types of binding sites. The primary one is probably responsible for the inhibition of
protein synthesis whereas the secondary one is probably related to the misreading and reading
tiirough of the messenger RNA.

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Two new partially saturated tricyclic ring systems, , a, 7, , , 10, 10a, 11-octahydro-11-oxodibenz [, ] oxepins (3a and 3), and -thiepins (a and ) were synthesized. Compounds a and were desulfurized to give a pair of isomeric 2-methylbenzoylcyclohexanes (10a and 10). Deuterated a and (11a and 11) were prepared starting from butadiene- (12). The stereochemical features of 3a (trans), 3 (cis), a (trans) and (cis) are compared with those of 10a, 10, 11a and 11 on the basis of proton nuclear magnetic resonance data.

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The group velocities of surface waves in the period range - s were measured to retrieve the anisotropy pattern of the surface wave velocities in the Eastern Pacific Ocean. WWSSN long period records at KIP, Hawaii, from ridge events in the Gulf of California, the East Pacific rise, and the Easter Island Cordillera were used to cover propagation directions N60°-N140°. These station and epicenters are in very favorable positions to minimize the effects of the lateral heterogeneity owing to ocean floor ages. The fast direction of the Rayleigh wave group velocity is normal to ridge, showing 3-% azimuthal anisotropy. The Love wave group velocity shows little azimuthal anisotropy. A model of a possible type of preferred orientation of constituent olivine beneath the Pacific Ocean is proposed, based on the elastic constants and the petrofabrics of dunite from Hidaka, Hokkaido, Japan, and that of olivine nodules in the ophiolite complex from the Bay of Islands and Oman. In this model, a-axis is concentrated along a particular direction and - and -axes form a girdle. Elastic constants of the model are =2.44, ==2.05, ==0.72, =0.67, =0.69, and = =0.75 (10¹² dyn·cm), where -axis, or the direction of the a-axis concentration, is normal to ridge and X₃-axis is vertical. If we assume that the type of the preferred orientation is an age-independent property of suboceanic lithosphere, this model can explain the overall features of the large-scale anisotropy in the Pacific Ocean: the azimuthal anisotropy of surface wave velocities as described above; the SH-SV anisotropy of surface waves of about 0.15 km/s; the P_n, anisotropy of 7. km/s to . km/s; the high S_m, velocity of about . km/s; and the ScS polarization anisotropy

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Examination of the secreted wax of a scale insect, Ceroplastes ceriferus has shown the presence of various compounds of considerable biogenetic interest. (a) The sesquiterpenes were enantiomeric with respect to those from . rubens, which infests the same host tree. Each insect may possess a specific set of - cyclases for the formation of sesquiterpenes. () Among the identified twelve diterpenes, two of labdane skeletons were the first naturally occurring compounds. () The sesterterpenes (-)(7-10) having 14-membered monocyclic skeletons were the first naturally occurring compounds being independent of the host tree. Remarkable is the finding of sesterterpenes resulting from different biogenetic cyclization mode from the same species, collected in different districts, or from the different species of the same genus. (d) The "cyclic" wax were the esters consisting of fatty acids (_<10>-_<32>) and unusual cyclic alcohols (diterpenes and sesterterpenes). Structures and stereochemistry of nine new sesterterpenes will be discussed on the basis of spectroscopic and chemical methods: Cericerol-I (), cericerol-II (2), 13-methoxy cericerene (3), 13-ethoxy cericerene (), α-cericerol-I (), cericeroic acid (), 18-dihydro-19-ol cericeroic acid (7), cericera-2(),(Z),10(),15(), 18-pentene-25-acid (), cericera-2(),(Z),10(),15(), 18-pentene-24-acid (), and cericerene-15,24-diol (10). Possibility of the configurational isomers for the structures ()and () at the position -24 and -25 are still remained to be elucidated. Bridged hydrocarbon (16) which was obtained by acid treatment of tosylate of cericerol-I () indicated the crown shape (A) for ().

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Six new cembranoids sarcophytol P (3), R (), S (), K (), F (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P (3) was shown to be the 20-hydroxy derivative of the major component sarcophytol A (), and afforded the cyclization product in CHCl_3 at room temperature, in a same way as in . Sarcophytols R () and S () were correlated to , by conversion of its 7R,R and 7S,S epoxide derivatives. Sarcophytol K() was a 13, 14-dihydroxycembranoid having a ,3Z-diene moiety. The absolute configuration of and its Z,3- and Z,3Z-isomers sarcophytol (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols F (11) and T (13) were - and ,3Z-isomers of . Compound 11 showed characteristic broadening of the ^H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols F (11, ,3), N (15, Z,3Z) and T (13,,3Z), spontaneous autoxidation-cyclization, in CHCl_3,was examined, in order to compare the stereochemical course of the reaction with that of (Z,3), which affords trans-fused bicyclo[.3.0]tetradecene systems. The ,3Z-isomer 13 gave the same cyclization product 18, as in the reaction of , even though it is isomeric at -,3. The Z,3Z isomer 15 gave 19, in consequence of the reversed geometry at -3 of 15. The ,3-isomer (11) gave the bicyclic product , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and , giving enantiomeric ketones 23 and 24 respectively. The (14)-epoxide 26 was shown to be the immediate precursor of , and acounted for the inversion of the geometry at - of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from . The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and , was controlled by the chirality of the epoxy rings. The -14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene (30), the parent hydrocarbon of , also reacted in CHCl_3 giving the bicyclic product 32.

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Eight new sterols, , -epidioxy-(, 24R)-23-methylergosta-, -dien-3β-ol (), 3β, , -trihydroxy-(, 24R)-23-methylergosta-7, -dien--one (2), 3β, , -trihydroxy-(24S)-ergost-7-en--one (3), 3β, , , 14α-tetrahydroxy-(, 24R)-ergosta-7, -dien--one (), (, 24R)-ergosta-7, -diene-3β, , , -tetrol (), , -epidioxy-3β-hydroxy-(, 24R)-ergosta-7, -dien--one (), , -epidioxy-3β-hydroxy-(24S)-ergost-7-en--one (7) and , -epoxy-(, 24R)-ergosta-, -diene-3β, 7β, 14α-triol (), have been isolated from five edible mushrooms, Lentinus edodes, Flammulina velutipes, Hypsizigus marmoreus, Pleurotus ostreatus and Pholiota nameko together with fifteen known ones (-23), of which two (16 and 17) are reported for the first time from a fungal source. The structures of these new compounds were elucidated on the basis of their spectral data.

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