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  • Suguru TAKATSUTO, Kiyomi KOBAYASHI, Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE
    Agricultural and Biological Chemistry
    1988年 52 巻 12 号 3217-3218
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE, Kiyomi KOBAYASHI, Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 8 号 2117-2118
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Keishi Hata, Fuyuki Sugawara, Naganori Ohisa, Saori Takahashi, Kazuyuki Hori
    Biological and Pharmaceutical Bulletin
    2002年 25 巻 8 号 1040-1044
    発行日: 2002年
    公開日: 2002/08/01
    ジャーナル フリー
    We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC
    3
    T
    3
    -
    E
    1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC
    3
    T
    3
    -
    E
    1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (
    22E
    ,24R)-ergosta-7,
    22
    -diene-
    ,
    ,
    -triol (1). 1 induced ALP activities of MC
    3
    T
    3
    -
    E
    1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC
    3
    T
    3
    -
    E
    1 cells by 1, ergosterol (
    2
    ), ergocalciferol (
    3
    ), cholesta-
    ,
    ,
    -triol (4), 7-dehydrocholesterol (
    5
    ) and cholecalciferol (
    6
    ) were tested. The enzyme activities of MC
    3
    T
    3
    -
    E
    1 cells were increased
    3
    .0-fold by 10 μM 1 and
    2
    .4-fold by 10 μM 4. However,
    2
    ,
    3
    ,
    5
    and
    6
    did not induce MC
    3
    T
    3
    -
    E
    1 cell ALP activity at 0.1—10 μM. These results suggested that the OH groups at C-
    5
    and/or C-
    6
    of 1 and 4 played an important role in their differentiation-inducing activities on MC
    3
    T
    3
    -
    E
    1 cells. Furthermore, 1 suppressed induction of MC
    3
    T
    3
    -
    E
    1 cell apoptosis by serum starvation.
  • Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 9 号 2361-2363
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Peter
    E
    . Hartmann, Leon R. Mitoulas, Jillian L. Sherriff
    日本食生活学会誌
    2000年 10 巻 4 号 65-73
    発行日: 2000/03/31
    公開日: 2011/01/31
    ジャーナル フリー
    Approximately
    99
    % of the fat in huma milk is secreted into the alveoli by the mammary secretory epithelial cell (lactocyte) in membrane bound milk fat globules (MFG). The MFG, unlike small molecules such as lactose, have little effect on the osmotic balance between milk and blood and therefore they can be stored in large amounts in the alveolar lumen.Approximately 98% of the fat in the MFG are tri-acylglycerols (TAG). Importantly, different species,
    e
    . g. women, rabbits, cows and elephants, have distinctive combinations of fatty acids esterified as TAG in their milk.We have measured 24-h milk production, fat content and fatty acid composition at 1,
    2
    , 4,
    6
    ,
    9
    , and 12 months of lactation in
    5
    women.Mean (±SD) milk production (375.
    5
    -1: 153.
    5
    mL/24-h/breast) differed between breasts, between women and with stage of lactation (p<0.05). Whereas the fat content (35.
    5
    ±7.86g/L) and the percentage composition of 18: 1n
    9
    (32.24±
    3
    .
    3
    ), 18:
    2
    n
    6
    (
    9
    .18±
    2
    .66), 18:
    3
    n
    3
    (0.76±0.21), 20: 4n
    6
    (0.37±0.07),
    22
    :
    5
    n
    3
    (0.17±0.04), and
    22
    :
    6
    n
    3
    (0.
    2
    ±0.07) differed only between women and with stage of lactation (p<0.05).In contrast, the amount delivered to the infant differed (p<0.05) between women only for 18:
    3
    n
    3
    ,
    22
    :
    5
    n
    3
    and
    22
    :
    6
    n
    3
    and no differences in amounts delivered were observed for any of these fatty acids from 1 to 12 months of lactation.Each child received a mean (±SD) of 8.27-
    2
    .84 g 18: 1n
    9
    ;
    2
    .38±0.98g 18:
    2
    n
    6
    ;194±73mg 18:
    3
    n
    3
    ;
    92
    ±31mg 20: 4n
    6
    ;43±14 mg
    22
    :
    5
    n
    3
    and 49±21 mg
    22
    :
    6
    n
    3
    every 24-h from breastmilk over the first year of life.These results indicate that variation in percentage composition of individual fatty acids (
    e
    .g.18:
    2
    n
    6
    ) does not always translate to variation in the amount delivered to the infant.
    Milk fat not only accounts for approximately 50% of the infant's energy intake, but also is responsible for the supply of the essential and long-chain polyunsaturated fatty acids that are required for the optimal development of the infant.For example, arachidonic acid (20: 4n
    6
    ) and eicosapentaenoic acid (20:
    5
    n
    3
    ) are essential precursors for the synthesis of prostaglandins and immunomodulatory eicosanoids.On the other hand, docosahexaenoic acid (
    22
    :
    6
    n
    3
    , DHA) is a major polyunsaturated fatty acid in the membranes of the cerebral cortex and retina and higher intakes of DHA have been associated with higher ratings in intelligence tests particularly in children born prematurely. Fatty acids and mono-acylglycerols released by hydrolysis of TAG in the infant's digestive tract have a detergent like lytic action and inactivate enveloped viruses, gram positive and gram negative bacteria, fungi and protozoa.The membrane surrounding the human MFG contains mucin filaments that may act as a decoy to pathogenic micro-organisms (
    e.g.E
    coli).Micro-organisms recognise mucin filaments as membrane docking sites from which to launch an invasive infection and are thereby lured away from the membrane docking sites on the epithelial cells lining the infant's digestive tract.
    Despite the importance of milk fat to the infant, it is the most variable component of human milk.It varies, over the course of a feed, over the course of the day, with stage of lactation, from one lactation to the next, between breasts, and between women. The major predictors of the fat content of milk over the course of a day have been shown to be the length of the interval between breastfeeds, the fat content at the end of the previous breastfeed, the amount of milk removed at the previous breastfeed and the amount of milk removed at the current feed.However, we have found that the fat content of milk is determined primarily by the amount of milk (degree of fullness) in the breast.For example, if the baby sleeps overnight, the mother's breasts will be full of milk in the morning and the fat content of fore-milk (milk obtained before a breastfeed) will be very low.
  • 榎 明潔, 八木 則男, 矢田部 龍一, 一本 英三郎
    土質工学会論文報告集
    1991年 31 巻 2 号 1-13
    発行日: 1991/06/15
    公開日: 2008/02/29
    ジャーナル フリー
    It has been shown after mathematical and mechanical investigation that Limit Equilibrium Method (LEM) can be considered as a method to obtain the necessary condition of Slip Line Method (SLM), and that the solution can be obtained under the condition that Fs=1 and ∂Fs/∂θ=O, where Fs is the safety factor and θ is the inclination of the plane on which the safety factor is defined. Next, the generalized LEM (GLEM) is proposed, dealing with the following points : (1) Triangular or quadrangular blocks can be treated; (
    2
    ) Safety factors are defined also on interblock planes; and (
    3
    ) All types of plastic problems, slope stability, bearing capacity, and earth pressure are identically formulated. Two situations regarding treatment of the moment equilibrium condition are discussed. GLEM is applied to well-known problems. The results agree well with those obtained by theoretical methods. GLEM can be used to obtain the distribution of earth pressure or bearing capacity. GLEM, Iike ordinary LEM, is very effective for the practical problems, but GLEM is more theoretical and it can result in more accurate solutions.
  • Sen-fang Sui, Erich Sackmann
    The Journal of Biochemistry
    1992年 111 巻 1 号 129-138
    発行日: 1992年
    公開日: 2008/11/18
    ジャーナル フリー
    In the first part of the present work the interaction of glycophorin with dimyristoylphosphatidylcholine (DMPC) is studied by freeze fracture electron microscopy, densitometry, calorimetry, and 90° static light scattering. An exothermic lipid/protein interaction energy of WP=190 kJ•mol-1 was found by application of the well known Van Laar relation for the displacement of the freezing point and the Gibbs-Duhem relationship. Secondly, the effects of
    Ca2
    + on the lipid/protein interaction were studied. Following
    Ca2
    + addition a remarkable decoupling of the interaction of the glycophorin head group with the bilayer surface was revealed by densitometry and gold-labeling electron microscopy. It is estimated that about 80% of lipid once disturbed by the adsorption of glycophorin head groups is decoupled after addition of
    Ca2
    +. Thirdly, the selective interaction of glycophorin with binary lipid mixtures was studied, including the mixtures of DMPC with dimyristoylphosphatidylserine (DMPS) and dilauroylphosphatidylcholine (DLPC), and the mixture of dipalmitoylphosphatidylcholine (DPPC) with DLPC.
  • 斉 洋之, 高津戸 秀, 池川 信夫, 田中 洋子, スミス コニー, デルカ F.ヘクター
    Chemical and Pharmaceutical Bulletin
    1984年 32 巻 10 号 3866-3872
    発行日: 1984/10/25
    公開日: 2008/03/31
    ジャーナル フリー
    Chemical synthesis of (
    22
    E
    , 24R)- and (
    22
    E
    , 24S)-1, 24-dihydroxy-
    Δ22
    -vitamin
    D3
    has been achieved starting with the commercially available dinorcholenic acid acetate. Synthesis involved introduction of the 1-hydroxy group by a reduction of the 1,
    2
    -epoxide generated by epoxidation of the 1, 4,
    6
    -trien-
    3
    -one. The side chain on the steroid was then constructed by means of a Wittig reaction followed by introduction of the Δ7 bond by standard methods and its protection with 1-phenyl-1,
    2
    , 4-triazoline-
    3
    ,
    5
    -dione. Subsequent reduction of the hydroxy groups in the steroid side chain followed by reduction of the Diels-Alder addition products yielded the both 24-isomers. The
    5
    , 7-dienes were irradiated and the corresponding vitamin D compounds isolated. Nuclear magnetic resonance was used to identify individual isomers. The (
    22
    E
    , 24S)-1, 24-hydroxyvitamin
    D3
    compound bound equally well to the chick intestinal cytosol receptor as 1, 25-dihydroxyvitamin
    D3
    , while the 24R-isomer was approximately ten times less active. In vivo, both isomers were less active than 1, 25-dihydroxyvitamin
    D3
    ; however, the 24S-isomer was considerably more active than the 24R-isomer approaching the activity of 1, 25-dihydroxyvitamin
    D3
    .
  • Thomas Efferth
    日本薬理学会年会要旨集
    2018年 WCP2018 巻 WCP2018_SY86-4
    発行日: 2018年
    公開日: 2020/09/10
    会議録・要旨集 オープンアクセス

    To combat complex systemic diseases that harbour robust biological networks such as cancer, single target intervention may be ineffective. Network pharmacology approaches are highly useful, because they differ from conventional drug discovery by addressing the ability of drugs to target numerous proteins or networks involved in a disease. Pleiotropic natural products are one of the promising strategies due to their multi-targeting and due to lower side effects. Here, we discuss the application of network pharmacology for cancer drug discovery. We provide an overview of the current state of knowledge on network pharmacology, focus on different technical approaches and implications for cancer therapy (

    e
    .g. polypharmacology and synthetic lethality), and illustrate the therapeutic potential with selected examples from herbal mixtures, medicinal herbs and isolated phytochemicals. Finally, we present future perspectives on their plausible applications for diagnosis and therapy of cancer.

    Selected papers: (1) Kadioglu O et al. Biochem Pharmacol. 2014;

    87
    :399-409. (
    2
    ) Zhao Q et al. Int J Cancer. 2015 ;137(
    6
    ):1446-56. (
    3
    ) Panossian et al. Phytomedicine. 2015;
    22
    :981-
    92
    . (4) Saeed M et al. J Nutr Biochem. 2015;26:44-56. (
    5
    ) Kadioglu O et al. Arch Toxicol. 2016;90:575-
    88
    . (
    6
    ) Poornima P et al. Pharmacol Res. 2016;111:290-302. (7) Efferth T et al. Oncotarget. 2017;8:50284-50304. (
    9
    ) Hong C et al. Sci Rep. 2017;7:11551. (10) Hamdoun S et al. Biochem Pharmacol. 2017;146:63-73.

  • 杉本 亘之, 高橋 正也
    日本養豚研究会誌
    1986年 23 巻 2 号 57-61
    発行日: 1986/06/30
    公開日: 2011/06/08
    ジャーナル フリー
    飼料の粒度と消化率との関係について, 現在配合飼料の主原料の一つであるトウモロコシを用いて検討した。
    粉砕機のスクリーンの穴径を1mm,
    2
    mm,
    3
    mmおよび
    5
    mmの4段階に設定し, 穀実トウモロコシをそれぞれ紛砕処理した。各粉砕トウモロコシは, 豚産肉能力検定飼料に40%づつ配合し, 試験飼料とした。供試豚は, ランドレース去勢雄
    5
    頭 (体重32~38kg) で, 消化試験は4種の試験飼料の他に基礎飼料として用いた配合飼料の
    5
    種類について
    5
    ×
    5
    のラテン方格法により, 予備期間
    5
    日, 採糞期間
    5
    日の全糞採取法により行なった。飼料の給与量は体重の4%とし, 1日に
    2
    回に分けて給与した。
    トウモロコシの消化率を1mm,
    2
    mm,
    3
    mmおよび
    5
    mm処理の順に示すと, 乾物90.
    3
    , 89.
    3
    ,
    88
    .8,
    87
    .
    6
    %, 有機物91.
    2
    , 90.1, 89.
    6
    ,
    88
    .4%, 粗蛋白質82.1, 74.
    9
    , 73.8, 70.
    6
    %, 粗脂肪84.
    3
    , 80.
    5
    , 74.
    6
    , 66.
    9
    %, NFE94.1, 93.
    9
    , 93.0,
    92
    .7%, 粗繊維19.
    5
    ,
    22
    .0, 27.
    6
    , 26.4%, エネルギー89.8,
    88
    .
    2
    ,
    87
    .
    5
    , 85.
    9
    %, 澱粉100.0,
    99
    .
    9
    ,
    99
    .7,
    99
    .
    6
    %であった。したがって, 粗繊維の消化率を除き, 各成分の消化率は粉砕粒度が大きくなるにつれ低下の傾向を示した。なお, 粗繊維以外の各成分の消化率は, 1mmと
    5
    mm処理との間にいずれも有意差 (P<0.05) がみられた。
    トウモロコシの乾物の栄養価を, 1mm,
    2
    mm,
    3
    mmおよび
    5
    mm処理の順に示すと, DCP7.7, 7.0,
    6
    .
    9
    ,
    6
    .7%, DE4.05, 4.05,
    3
    .94,
    3
    .93kcal/g, TDN94.
    3
    , 93.1,
    92
    .1, 90.4%であり, 1mmと
    5
    mm処理との間に, いずれの栄養価とも有意差 (P<0.05) が認められた。なお, DCPおよびTDNについては, 1mmと
    3
    mm処理との間にも有意差 (P<0.05) が認められた。
    以上の結果, 粒度が消化率に大きな影響を及ぼすことから, 飼料の消化率および栄養価を精度よく比較検討する上で, 飼料の粒度表示も重要な要因になるものと考えられた。
  • Kazuo TAKEUCHI, Masayuki ATSUCHI, Shoji ODA, Minoru JINBO, Takeshi MASUI, Motohide OGASHIWA
    Neurologia medico-chirurgica
    1968年 10 巻 132-133
    発行日: 1968年
    公開日: 2007/08/17
    ジャーナル フリー
  • Paulo F Silveira, Patricia L Alves, Rafaela F Alponti
    日本薬理学会年会要旨集
    2018年 WCP2018 巻 WCP2018_PO3-6-14
    発行日: 2018年
    公開日: 2020/09/10
    会議録・要旨集 オープンアクセス

    Background: Exenatide (EXE), a prototypical GLP-1R agonist, is known as glucoregulator, antiobesogenic and antidyslipidemic in hyperlipidic and hyperglucidic diet-induced obesity in rats (DIO). Objective: To evaluate bone effects of DIO and DIO treatment with EXE (DIO-

    E
    ). Methods: 72-75-day-old male rats had access only to (i) hyperlipidic food (
    5
    .
    2
    kcal/g) and 30% sucrose solution for drinking (1.
    2
    kcal/mL), or (ii) received normocaloric diet (
    3
    kcal/g) and were allowed to feed and to drink water ad libitum. 122-125-day-old rats with 20% overweight were selected from i as obese and those with normal weight were selected from ii as control (C) animals. Thus, obese animals remained untreated (DIO) or were treated with 10 μg EXE/kg sc (DIO-
    E
    ) daily, for 20 days. Plasma levels of insulin (INS), leptin (LEP), osteocalcin (OCN), carboxy-terminal telopeptide of type 1 collagen (CTX-1) (ng/mL), as well as calcitonin (CT) and procollagen type 1 amino-terminal propeptide (P1NP) (pg/mL) were measured by ELISA. Bone mineral density of femur (BMDF) (g/
    cm3
    ) was measured by X-rays. Results: DIO exhibited similar INS (12.
    9
    ±1.83, n=4) and CT (
    2
    .80±1.05, n=4), higher LEP (0.33±0.04, n=
    5
    ) and lower CTX-1 (0.48±0.
    22
    , n=
    3
    ) than C. The treatment of DIO with EXE restored LEP (0.04±0.02, n=
    5
    ), decreased CTX-1 (0.50±0.
    22
    , n=
    5
    ) and increased CT (
    6
    .
    87
    ±0.72, n=
    5
    ). The levels of OCN (1.58±0.41, n=
    5
    ), P1NP (
    92
    .64±16.89, n=
    5
    ) and BMDF (1.
    9
    ±0.07, n=
    5
    ) of C and those of DIO and DIO-
    E
    were similar. Conclusions: Despite of increased LEP, decreased CTX-1 and normal OCN, P1NP and BMDF reflect a relative normal balance in bone turnover in DIO. Mechanical overloading due to high body mass is known as a factor that promotes this normal condition. Given that EXE decreases 14% body mass of DIO, the present study suggests that normalization of LEP and increased CT and decreased CTX-1 are concomitant beneficial effects of EXE that contribute for maintaining bone turnover under decreased mechanical load in DIO.

    Supported by FAPESP, CNPq and CAPES

  • Yasunori YAOITA, Keiko AMEMIYA, Hiroyuki OHNUMA, Katsuyuki FURUMURA, Akihiro MASAKI, Toshihiko MATSUKI, Masao KIKUCHI
    Chemical and Pharmaceutical Bulletin
    1998年 46 巻 6 号 944-950
    発行日: 1998/06/15
    公開日: 2008/03/31
    ジャーナル フリー
    Eight new sterols,
    , 8α-epidioxy-(
    22
    E
    , 24R)-23-methylergosta-
    6
    ,
    22
    -dien-
    -ol (1),
    ,
    ,
    -trihydroxy-(
    22
    E
    , 24R)-23-methylergosta-7,
    22
    -dien-
    6
    -one (
    2
    ),
    ,
    ,
    -trihydroxy-(24S)-ergost-7-en-
    6
    -one (
    3
    ),
    ,
    ,
    , 14α-tetrahydroxy-(
    22
    E
    , 24R)-ergosta-7,
    22
    -dien-
    6
    -one (4), (
    22
    E
    , 24R)-ergosta-7,
    22
    -diene-
    ,
    ,
    ,
    -tetrol (
    5
    ),
    ,
    -epidioxy-
    -hydroxy-(
    22
    E
    , 24R)-ergosta-7,
    22
    -dien-
    6
    -one (
    6
    ),
    ,
    -epidioxy-
    -hydroxy-(24S)-ergost-7-en-
    6
    -one (7) and
    ,
    -epoxy-(
    22
    E
    , 24R)-ergosta-8,
    22
    -diene-
    , 7β, 14α-triol (8), have been isolated from five edible mushrooms, Lentinus edodes, Flammulina velutipes, Hypsizigus marmoreus, Pleurotus ostreatus and Pholiota nameko together with fifteen known ones (
    9
    -23), of which two (16 and 17) are reported for the first time from a fungal source. The structures of these new compounds were elucidated on the basis of their spectral data.
  • 佐藤 昭子, 田河 清, 池 康嘉, 三橋 進
    Japanese Journal of Medical Science and Biology
    1980年 33 巻 3 号 185-188
    発行日: 1980年
    公開日: 2010/03/19
    ジャーナル フリー
    Two hundred and eighty-seven drug-resistant strains of Escherichia coli were isolated from 150 ground meat samples of chicken purchased at markets in 1978, and 210 R plasmids were demonstrated in those strains.
    The most frequent resistance pattern (
    88
    %) included SA or TC resistance. The pattern associated with SM resistance was secondly frequent and the frequency of CM resistance was as low as 20%. In relation to TC, CM, SM, and SA, the isolation frequency was the highest with strains triple resistant to any combination of three of four drugs. Among the resistant
    E
    coli
    strains, R plasmids with single resistance to each of four drugs and those accompanying KM or APC resistance were most frequently demonstrated.
    The use of antibiotics has brought many advantages to the livestock industry. The drugs not only cure the animals of bacterial infections but also accelerate growth and the increase in the body size. However, long-term administration of antibiotics has brought an increase in the number of drug-resistant organisms, especially those with conjugally transferable resistance. This paper deals with the studies on the distribution of drug resistance plasmids among
    E
    . coli
    strains isolated from chickens purchased at commercial markets.
    We isolated 287
    E
    . coli
    strains resistant to any one or any combination of the drugs tested, i.
    e
    ., TC, CM, SM, SA, KM and APC. Drug-resistant
    E
    . coli
    strains were isolated from all chicken materials tested. SA-and TC-resistant strains were isolated most frequently (
    87
    .8% and
    87
    .
    5
    %, respectively), followed by SM- (68.
    6
    %) and KM-resistant ones (41.8%) . The isolation frequencies of CM- and APC-resistant strains were very low, being
    22
    .0% and
    22
    .
    3
    %, respectively.
  • Yasunori YAOITA, Makiko ENDO, Yoshino TANI, Kaori MACHIDA, Keiko AMEMIYA, Katsuyuki FURUMURA, Masao KIKUCHI
    Chemical and Pharmaceutical Bulletin
    1999年 47 巻 6 号 847-851
    発行日: 1999/06/15
    公開日: 2008/03/31
    ジャーナル フリー
    Six new sterols,
    ,
    ;8α,
    -diepoxy-(
    22
    E
    , 24R)-ergost-
    22
    -ene-
    , 7α-diol (1),
    ,
    ;8α,
    -diepoxy-(
    22
    E
    , 24R)-ergost-
    22
    -ene-
    , 7β-diol (
    2
    ),
    ,
    -epoxy-(
    22
    E
    , 24R)-ergosta-8,
    22
    -diene-
    , 7β-diol (
    3
    ), (
    22
    E
    , 24R)-23-methylergosta-7,
    22
    -diene-
    ,
    ,
    -triol (4), (
    22
    E
    , 24R)-23-methulergosta-7,
    22
    -diene-
    ,
    ,
    ,
    -tetrol (
    5
    ) and (24S)-ergost-7-ene-
    ,
    ,
    ,
    -tetrol (
    6
    ), have been isolated from seven mushrooms, Amanita pantherina, Amantia virgineoides, Lactarius piperatus, Lyophyllum shimeji, Tricholoma portentosum, Hypsizigus marmoreus and Lentinula edodes together with eighteen known ones (7-24). The structures of these new compounds were elucidated on the basis of their spectral data.
  • 百目鬼 郁男, 中原 達夫, 山内 亮
    家畜繁殖研究會誌
    1974年 20 巻 2 号 76-80
    発行日: 1974/08/30
    公開日: 2008/05/15
    ジャーナル フリー
    性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
    Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-
    2
    AおよびType203で測定した結果,励起光538nm,螢光552.
    5
    nmであった。この螢光特性は
    E1
    ,
    E2
    および
    E3
    にそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•
    5
    ±
    22
    5nm
    を読み,ALLENの補正を行なった。この条件において
    E1
    ,
    E2
    および
    E3
    -methyletherの最少検出量は1ngであった。回収率補正の目的で加えた
    6
    ,7-
    3H
    -
    E2
    -17βの全過程における回収率は平均60.
    3
    ±11.7%であった。正常性周期を示す黒毛和種
    2
    頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共
    E1
    ,
    E2
    の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,
    E2
    E1
    にくらべ全般に高値であった。また
    E3
    は検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.
    3
    および
    99.8ng
    /l;
    E15.9
    および16.0ng/l,
    E229.4
    および83.8ng/l)を形成し,排卵後は急激に減少して最低値(
    3
    .8~
    5.3ng
    /l;
    E11.6
    および
    1.9ng
    /l,
    E22.2
    および
    3.4ng
    /l)を示した。黄体期の最高値(10.1および27.0ng/l;
    E12.4
    および
    3.4ng
    /l,
    E27.7
    および
    23.6ng
    /l)は排卵後
    6
    ~8日に認めた。すなわちestrogenの血中濃度は性周期の間に
    2
    つのピークを形成することを認めた。
  • 吉良 正道
    日本内分泌学会雑誌
    1980年 56 巻 10 号 1461-1474
    発行日: 1980/10/20
    公開日: 2012/09/24
    ジャーナル フリー
    Testosterone (T) and estradiol (
    E2
    ) are bound to serum proteins, and it is believed that this binding affects the biological activity of these hormones. Testosterone-estradiol binding globulin (TeBG) is the primary β-globulin which binds both T and
    E2
    specifically.
    In recent years, various assay methods for determining TeBG capacity (TeBGC) and %-bound T,
    E2
    to TeBG (%T-TeBG, %
    E2
    -TeBG) have been developed but most of them too laborious and complex to apply in practice.
    We have developed a rapid and simplified method to determine TeBGC and %T,
    E2
    -TeBG by means of a modified ammonium sulfate precipitation method;
    1) Assay method for TeBGC
    (1) to a series of assay tubes, add
    3
    H-DHT and
    5
    ng DHT (control; 500ng DHT)
    (
    2
    ) dry up under gentle
    N2
    gas stream at 40°C
    (
    3
    ) add 0.1 ml of undiluted serum (both male and female) or diluted serum (pregnant) to the appropriate tube, and allow to incubate at 37°C for 30 min.
    (4) add 0.
    5
    ml of ice-cold 60%-saturated ammonium sulfate
    (
    5
    ) keep in ice bath for 15 min.
    (
    6
    ) centrifuge at 1500 xg at 0°C for 30 min.
    (7) count 0.
    2
    ml supernatant and calculate
    2
    ) Assay method for %T,
    E2
    -TeBG
    (1) to a series of assay tubes, add
    3
    H-T or
    3
    H-
    E2
    (control; with 500 ng T or
    E2
    ) Steps (
    2
    ), (
    3
    ), (4), (
    5
    ), (
    6
    ) and (7) are processed as in the TeBGC method except using undiluted serum in every case.
    The values obtained were
    9
    .
    9
    ±
    3
    .4 (mean ± SD) for male (expressed as ng DHT/ml), 15.
    3
    ±
    3
    .
    9
    for female and
    87
    .1 ± 16.
    9
    for pregnant. While %T-TeBG was 61.
    6
    ±
    5
    .1% for male, 76.8 ±
    2
    .8% for female, and
    92
    .4 ± 4.
    3
    % for pregnant, %
    E2
    -TeBG was 25.0 ± 4.0% for male, 48.1 ±
    3
    .4% for female and 80.
    3
    ±
    6
    .
    3
    % for pregnant.
    The values thus obtained for %T-and %
    E2
    -TeBG by the author's ammoniuum sulfate precipitation method are highly correlated with those which are determined by the equilibrium dialysis method. Correlation coefficients are 0.
    99
    for %T-TeBG and 0.97 for %
    E2
    -TeBG.
  • Masuko Kobori, Mitsuru Yoshida, Mayumi Ohnishi-Kameyama, Toshiyuki Takei, Hiroshi Shinmoto
    Biological and Pharmaceutical Bulletin
    2006年 29 巻 4 号 755-759
    発行日: 2006年
    公開日: 2006/04/01
    ジャーナル フリー
    We purified a sterol with antitumor activity from the edible mushroom Sarcodon aspratus (BERK.) S. ITO and identified it as
    ,8α-epidioxy-
    22E
    -ergosta-
    6
    ,
    9
    (11),
    22
    -trien-
    -ol (
    9
    ,11-dehydroergosterol peroxide (
    9
    (11)-DHEP)). Purified
    9
    (11)-DHEP was a more effective inhibitor of HL60 leukemia cell growth and stronger apoptosis-inducer than
    ,8α-epidioxy-
    22E
    -ergosta-
    6
    ,
    22
    -dien-
    -ol (ergosterol peroxide (EP)) that we had previously identified as an apoptosis inducer. Moreover,
    9
    (11)-DHEP selectively suppressed the growth of HT29 human colon adenocarcinoma cells but not WI38 normal human fibroblasts. After
    5
    d incubation of HT29 with 7 μM
    9
    (11)-DHEP, the number of S phase cells decreased from 23 to 15% of total diploid cells and 17% became hypodiploid. Expression of the cyclin-dependent kinase inhibitor 1A (p21, WAF1, Cip1) (CDKN1A), which has been shown to cause cell cycle arrest and apoptosis in HT29 cells, was induced by
    9
    (11)-DHEP. These results suggest that
    9
    (11)-DHEP inhibits HT29 cell growth by inducing CDKN1A expression, thus causing cell cycle arrest and apoptosis.
  • 臺灣平埔族の人類學的研究VI
    余 錦泉
    人類學雜誌
    1942年 57 巻 4 号 163-176
    発行日: 1942/03/28
    公開日: 2008/02/26
    ジャーナル フリー
    Die Hauptresultate der Untersuchungen der Fingermustern bei 310 Peipo von Rato werden wie folgt zusammengef asst:
    Prädominierende Formeln für die Leistenbilder auf den ganzen fünf Fingern :
    Figura tensa auf den allen funf Fingern;(
    6
    .
    9
    ±0.46)nf Fingern;
    w0(11.8±1.30), w1(12.
    6
    ±1.33),
    w2
    (16.
    5
    ±1.49),
    w3
    (16.0±1.47), w4(21.
    3
    ±1.64),
    w5
    (21.
    9
    ±1.66), w0-1(24.4±1.72),
    w2
    -
    3
    (32.4±1.
    88
    ), w4-
    5
    (43.
    2
    ±1.
    99
    ),
    Figurae tactiles des Grossfingern; a(0.
    3
    ±0.
    22
    ), r(0.
    5
    ±0.28), u(33.4±1.89), w(65.8±1.91),
    Figurae tactiles des Zeigefingers; a(
    3
    .4±0.73), r(10.
    2
    ±1.
    22
    ), u(21.8±1.66), w(64.4±1.
    92
    ),
    Figurae tactiles des Mittelfingers; a(1.8±0.53), r(1.
    9
    ±0.55), u(41.
    3
    ±1.98), w(54.8±
    2
    .00),
    Figurae tactiles des Ringfingers; a(0.
    3
    ±0.
    22
    ), r(0.
    2
    ±0.18), u(29.8±1.84), w(69.
    5
    ±1.85),
    Figurae tactiles des Kleinfingers; a(0.
    5
    ±0.28), r(0.
    2
    ±0.18), u(65.
    5
    ±1.91), w(33.7±1.90),
    Figurae tacties auf allen fünf Fingern; a(1.
    3
    ±0.20), r(
    2
    .
    6
    ±0.29), u(38.4±0.
    87
    ), w(57.
    6
    ±0.89),
    Sinus duplicatus auf den allen fünf Fingern;(15.
    2
    ±0.85),
    Figura tensa auf den allen funf Fingern;(
    6
    .
    9
    ±0.46)
  • Sultan Samran Al-Lihaibi, Seif-Eldin Nasr Ayyad, Fekry Shaher, Walied Mohamed Alarif
    Chemical and Pharmaceutical Bulletin
    2010年 58 巻 12 号 1635-1638
    発行日: 2010/12/01
    公開日: 2010/12/01
    ジャーナル フリー
    From the black coral Antipathies dichotoma, a sphingolipid (
    2S
    *,
    3S
    *,
    4E
    ,
    8E
    )-
    2N
    -[tetradecanoyl]-4(
    E
    ),8(
    E
    )-icosadiene-1,
    3
    -diol (1) and a steroid (
    22E
    )-methylcholesta-
    5
    ,
    22
    -diene-1α,
    ,7α-triol (
    2
    ) were isolated. Other known compounds,
    ,7α-dihydroxy-cholest-
    5
    -ene (
    3
    ), (
    22E
    ,24S),
    ,8α-epidioxy-24-methylcholesta-
    6
    ,
    22
    -dien-
    -ol (4) and (
    22E
    ,24S),
    ,8α-epidioxy-24-methylcholesta-
    6
    ,
    9
    (11),
    22
    -trien-
    -ol (
    5
    ). The structures were established on the basis of NMR spectroscopic analysis and comparison with literature. The antibacterial activity of five compounds was evaluated.
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