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  • Suguru TAKATSUTO, Kiyomi KOBAYASHI, Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE
    Agricultural and Biological Chemistry
    1988年 52 巻 12 号 3217-3218
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Mitsunobu Ono, Masanobu Shindoh, Masanobu Kobayashi, Fumihiro Higashino, Takao Kohgo, Koichi Yoshida, Yasunori Totsuka
    Oral Medicine & Pathology
    2004年 9 巻 1 号 19-24
    発行日: 2004/03/25
    公開日: 2008/04/01
    ジャーナル フリー
    E
    1
    AF
    , an ets-oncogene family transcription factor, has been shown to upregulate transcription from matrix metalloproteinase (MMP) genes, which confers the invasive phenotype on cancer cells. On the other hand, we have demonstrated by luciferase assay that
    E
    1
    AF
    positively regulates transcription from the p21waf1/cip1 promoter. Ca
    9
    .
    22
    cells were transfected with a metallothionein-inducible
    E
    1
    AF
    expression vector (Ca
    9
    .
    22
    MTF) to investigate the relationship between invasive ability and cell cycle modulation caused by
    E
    1
    AF
    . MMP-
    9
    and p21waf1/cip1 protein were synergistically increased in the ZnCl2 stimulated CA
    9
    .
    22
    MTF cells, and both proteins were identified in the same cells when
    E
    1
    AF
    was induced. These results imply that invasive growth of tumor cells occurs in the static state of the cell cycle and that
    E
    1
    AF
    plays a key role in this phenomenon.
  • Sook Kyoung Kim, Kyoung-Jin Shin, Boyoung Joung, Moon-Hyoung Lee, Hui-Nam Pak
    Journal of Arrhythmia
    2011年 27 巻 Supplement 号 OP42_4
    発行日: 2011年
    公開日: 2012/03/14
    ジャーナル フリー
    Backgrounds: It has been reported that somatic mutations of mitochondrial DNA (mtDNA) is related with aging, or development of cardiovascular disease. We hypothesized that mtDNA4977 deletion mutation (4977-) detected from human blood contributes to atrial dysfunction in patients with
    AF
    . Methods: We studied for mtDNA4977- in 183 patients with non-valvular
    AF
    , (127 PAF, 56 PeAF) and 69 patients with healthy control. The detection of mtDNA4977- was determined by Gene Scan. Results: 1. The mtDNA4977- in
    AF
    patients were older than those without mutation (61.4±
    9
    .2 vs. 51.8±
    9
    .
    9
    , p<0.0001). 2. Frequency of mtDNA4977- mutation in patients with
    AF
    ≤45 years old was not different to age matched control (7.
    3
    % vs. 11.
    6
    %, p=0.2371).
    3
    . The mtDNA4977- had higher
    E
    /
    E
    , (11.
    3
    ±4.8 vs.
    9
    .1±
    3
    .1, p=0.0005), plasma levels of TIMP-1 (1.51±0.77 vs. 1.28±0.57 ng/mL, p=0.0210) and ANP (
    3
    .11±2.67 vs. 1.91±2.01 nmole/L, p=0.0010), and were more frequently taking statin (27.2% vs.
    22
    .4%, p=0.0055) than those without mutation. 4.
    82
    .
    3
    % with mtDNA4977- and 85.5% without mtDNA4977- remain in sinus rhythm 12.7±5.
    9
    months after RFCA (p=0.3008). Conclusion: mtDNA4977- was related with aging, diastolic dysfunction, serologic markers related with atrial remodeling or pressure overloading. Its frequency was not different to age matched patients with control, and did not affect the clinical outcome after RFCA.
  • Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE, Kiyomi KOBAYASHI, Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 8 号 2117-2118
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Keishi Hata, Fuyuki Sugawara, Naganori Ohisa, Saori Takahashi, Kazuyuki Hori
    Biological and Pharmaceutical Bulletin
    2002年 25 巻 8 号 1040-1044
    発行日: 2002年
    公開日: 2002/08/01
    ジャーナル フリー
    We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC
    3
    T
    3
    -
    E
    1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC
    3
    T
    3
    -
    E
    1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (
    22E
    ,24R)-ergosta-7,
    22
    -diene-
    ,5α,
    -triol (1). 1 induced ALP activities of MC
    3
    T
    3
    -
    E
    1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC
    3
    T
    3
    -
    E
    1 cells by 1, ergosterol (2), ergocalciferol (
    3
    ), cholesta-
    ,5α,
    -triol (4), 7-dehydrocholesterol (5) and cholecalciferol (
    6
    ) were tested. The enzyme activities of MC
    3
    T
    3
    -
    E
    1 cells were increased
    3
    .0-fold by 10 μM 1 and 2.4-fold by 10 μM 4. However, 2,
    3
    , 5 and
    6
    did not induce MC
    3
    T
    3
    -
    E
    1 cell ALP activity at 0.1—10 μM. These results suggested that the OH groups at C-5 and/or C-
    6
    of 1 and 4 played an important role in their differentiation-inducing activities on MC
    3
    T
    3
    -
    E
    1 cells. Furthermore, 1 suppressed induction of MC
    3
    T
    3
    -
    E
    1 cell apoptosis by serum starvation.
  • *山下 正道
    日本薬理学会年会要旨集
    2021年 94 巻 94_1-P2-25
    発行日: 2021年
    公開日: 2021/03/21
    会議録・要旨集 オープンアクセス

    Effect of an antirheumatic gold compound, Auranofin (

    AF
    ), have been revealed.  1-10 µM of
    AF
    inhibited prostaglandin
    E2
    (PGE2) production and nitric oxide (NO) production in macrophages treated with some stimulatory compounds.  We found that
    AF
    reduced cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) mRNA levels, but not affected their enzyme activities.  We then revealed that
    AF
    partly inhibited the nuclear-translocation of NF-κB while PGE2 production was almost completely inhibited.

      In the review on manuscripts,

    AF
    is reported to down-regulate the mRNA levels of SLC47A1,
    SLC22A1
    ,
    ATP1A3
    , ABCG2, SLCO1B1,
    PIK3CA
    , and up-regulate the mRNA levels of
    ABCC3
    , ABCB1,
    SLC22A3
    , ABCC2, SLC31A2, CLTD, CLTB.  PGs are known to be transported by 4 transporters, OATP-PG (SLC
    22
    A
    22
    ), SLC21A2 (OATP2A1, SLCO2), SLC21A11 (OATP
    3
    A1), and ABCC4, though I could not identify whether
    AF
    reduced PGE2 concentration in cellular cultured medium as described above via these transporters by downregulation for export of PGE2.

  • Sen-fang Sui, Erich Sackmann
    The Journal of Biochemistry
    1992年 111 巻 1 号 129-138
    発行日: 1992年
    公開日: 2008/11/18
    ジャーナル フリー
    In the first part of the present work the interaction of glycophorin with dimyristoylphosphatidylcholine (DMPC) is studied by freeze fracture electron microscopy, densitometry, calorimetry, and 90° static light scattering. An exothermic lipid/protein interaction energy of WP=190 kJ•mol-1 was found by application of the well known Van Laar relation for the displacement of the freezing point and the Gibbs-Duhem relationship. Secondly, the effects of Ca2+ on the lipid/protein interaction were studied. Following Ca2+ addition a remarkable decoupling of the interaction of the glycophorin head group with the bilayer surface was revealed by densitometry and gold-labeling electron microscopy. It is estimated that about 80% of lipid once disturbed by the adsorption of glycophorin head groups is decoupled after addition of Ca2+. Thirdly, the selective interaction of glycophorin with binary lipid mixtures was studied, including the mixtures of DMPC with dimyristoylphosphatidylserine (DMPS) and dilauroylphosphatidylcholine (DLPC), and the mixture of dipalmitoylphosphatidylcholine (DPPC) with DLPC.
  • 斉 洋之, 高津戸 秀, 池川 信夫, 田中 洋子, スミス コニー, デルカ F.ヘクター
    Chemical and Pharmaceutical Bulletin
    1984年 32 巻 10 号 3866-3872
    発行日: 1984/10/25
    公開日: 2008/03/31
    ジャーナル フリー
    Chemical synthesis of (
    22
    E
    , 24R)- and (
    22
    E
    , 24S)-1, 24-dihydroxy-
    Δ22
    -vitamin
    D3
    has been achieved starting with the commercially available dinorcholenic acid acetate. Synthesis involved introduction of the 1-hydroxy group by a reduction of the 1, 2-epoxide generated by epoxidation of the 1, 4,
    6
    -trien-
    3
    -one. The side chain on the steroid was then constructed by means of a Wittig reaction followed by introduction of the Δ7 bond by standard methods and its protection with 1-phenyl-1, 2, 4-triazoline-
    3
    , 5-dione. Subsequent reduction of the hydroxy groups in the steroid side chain followed by reduction of the Diels-Alder addition products yielded the both 24-isomers. The 5, 7-dienes were irradiated and the corresponding vitamin D compounds isolated. Nuclear magnetic resonance was used to identify individual isomers. The (
    22
    E
    , 24S)-1, 24-hydroxyvitamin
    D3
    compound bound equally well to the chick intestinal cytosol receptor as 1, 25-dihydroxyvitamin
    D3
    , while the 24R-isomer was approximately ten times less active. In vivo, both isomers were less active than 1, 25-dihydroxyvitamin
    D3
    ; however, the 24S-isomer was considerably more active than the 24R-isomer approaching the activity of 1, 25-dihydroxyvitamin
    D3
    .
  • Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 9 号 2361-2363
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Jae-Sun Uhm, Boyoung Joung, Hye Jin Hwang, Jong-Youn Kim, Moon-Hyoung Lee, Hui-Nam Pak
    Journal of Arrhythmia
    2011年 27 巻 Supplement 号 OP58_2
    発行日: 2011年
    公開日: 2012/03/27
    ジャーナル フリー
    Backgrounds: We evaluated that electrophysiological characteristics and clinical outcomes of atrial fibrillation (
    AF
    ) with sinus node dysfunction (SND) after radiofrequency catheter ablation (RFCA). Methods: Among 428 patients (male, 77.
    3
    %; 56.0±11.0 years old; paroxysmal
    AF
    , 69.4%) who underwent RFCA for
    AF
    , 50 patients (11.7%) who had ECG documented symptomatic SND (28 Tachybrady (T-Bs) and
    22
    sick sinus syndrome; SSS) were compared with 378 patients without SND. Results: 1. The patients with SND were older (60.
    3
    ±61617;
    9
    .7 vs. 55.4±61617; 11.1 years, p=0.003) and had higher
    E
    /
    E
    ′ (12.4±61617; 5.1 vs.
    9
    .
    9
    ±61617; 4.1, p=0.002) than those without SND. 2. Effective refractory period (ERP) at high right atrium (RA) was longer (ERP; 244.1±61617; 26.1 vs. 232.2±61617; 28.2 ms, p=0.033) and endocardial voltage at posterior left atrium (LA) was higher (1.2±61617; 0.8 vs. 0.
    9
    ±61617; 0.8 mV, p=0.041) in patients with SND than those without it.
    3
    . The recurrence rate was not different significantly between groups (28.0% vs. 20.0%, p=0.623) during 12.7±5.
    9
    months. A patient with SSS (4.5%) and a patient with T-Bs (
    3
    .
    6
    %) were implanted pacemaker because of recurrent symptomatic bradycardia after RFCA. Conclusion:
    AF
    is associated with SND more often in patients with old age, diastolic dysfunction, and high RA ERP. RFCA results in comparable clinical outcome in those patients, but 4% of them required pacemaker implantation due to
    AF
    recurrence and symptomatic bradycardia.
  • R. I.・Ittrich-Fluorometry
    田中 啓幹
    日本泌尿器科學會雑誌
    1969年 60 巻 12 号 1125-1142
    発行日: 1969年
    公開日: 2010/07/23
    ジャーナル フリー
    Urinary estrogens have been assayed chiefly with the Kober hydroquinone colorimetry or sulfuric acid fluorimetry. However, it is difficult to determine accurately small amounts of estrogens such as in urines from males, oophorectomized women or menopausal women. In this study, male urinary estrogens were determined by a modification of the method reported by Kambegawa and some experimental studies were made on male urines. 200ml aliquot of 24-hour urine is hydrolyzed by the
    3
    -step hydrolysis, added with estrone-
    6
    , 73H, 17β-estradiol-
    6
    , 73H and estriol-
    6
    , 73H, and extracted with ether, followed by alkaline washes, methylation by a supersonic wave mixer, thin layer and column chromatography, liquid scintillation spectrometry and the Ittrich fluorometry. Results of experimental studies and estimation of urinary estrogens in males are as follows: 1) After the
    3
    -step hydrolysis, 54.5% of the urinary estrogens were collected as the glucuronide, 30% in the solvolyzed fraction and 15.5% as the hot acid hydrolyzate. 2) The methylation of estrogens was achieved 10-20% better by the use of the supersonic wave mixer than by a magnetic stirrer.
    3
    ) Calculating efficiency of the Packard Tri-carb liquid scintillation spectrometer was tested in varieties of the estrogen concentrations using the dilution and the external standardization methods. There was noted no influence of the estrogen concentrations on the counting efficiency in the determination ranges. 4) In the range of 2×10-
    3
    -1.0μg, a linear relationship on the standard curve was obtained applying the Allen's correction, when fluorometry was conditioned with the excitation light wave length of 510mμ and the fluorescence wave lengths of 530, 550 and 570mμ for each of the three estrogen-methyl ethers. 5) Urinary estrogens were determined in 15 normal males. In 5 subjects (2-13 years old) the values were:
    E1
    : 1.06±1.09;
    E2
    : 0.64±0.54;
    E3
    : 0.76±0.68 and the total
    E
    : 2.46±1.99μg/24hr. In 5 men (
    22
    -38 years old),
    E1
    : 5.02±1.57;
    E2
    : 2.58±0.21;
    E3
    : 4.11±1.
    82
    and the total
    E
    : 11.72±2.97μg/24hr. In 5 men (46-72 years old),
    E1
    :
    3
    .81±0.31;
    E1
    : 1.71±0.48;
    E3
    :
    3
    .51±0.
    94
    and the total
    E
    :
    9
    .03±1.33μg/24hr.
    6
    ) Urinary estrogens were also determined in patients with a variety of clinical syndromes.
  • Nobutoshi TANAKA, Ken-ichi HOSOI, Daizo TANAKA, Mimei TAKAHASHI
    Chemical and Pharmaceutical Bulletin
    1996年 44 巻 4 号 843-846
    発行日: 1996/04/15
    公開日: 2008/03/31
    ジャーナル フリー
    Chemical properties of ergosta-4,
    6
    , 8(14),
    22
    -tetraen-
    3
    -one (1) were investigated. Though 1 is rather stable to acids or bases, it reacts easily with two moleculres of oxygen on irradiation with UV light to give
    ,
    -epidioxy-14α-hydroperoxyergosta-4, 7,
    22
    -trien-
    3
    -one (2), which is transformed successively to
    , 7α;8α,
    -diepoxy-14α-hydroperoxyergosta-4,
    22
    -dien-
    3
    -one (
    3
    ) and 14α-hydroperoxy-
    -hydroxyergosta-4, 7,
    22
    -triene-
    3
    ,
    6
    -dione (4) under these reaction conditions.
  • 伊藤 大介
    日本物理学会講演概要集
    1997年 52.2.1 巻 22a-E-9
    発行日: 1997/09/02
    公開日: 2018/03/04
    会議録・要旨集 フリー
  • Hideyuki Hara, Masahiro Yoshinaga, Yumie Matsui, Satoshi Yamamoto, Yuzo Akita, Takahiro Ishido, Bin Tsukada, Kotaro Yutaka, Keita Horitani, Masahiro Karakawa
    Journal of Arrhythmia
    2011年 27 巻 Supplement 号 OP11_5
    発行日: 2011年
    公開日: 2012/02/02
    ジャーナル フリー
    Objective: Our objective was to evaluate the mechanism of atrial tachycardia (AT) induced by atrial pacing using EnSite NavX multi-electrode mapping system after extensive encircling pulmonary vein isolation (EEPVI) for the paroxysmal atrial fibrillation (
    AF
    ). Methods: We evaluated 100 consecutive paroxysmal
    AF
    patients undergoing EEPVI (all were first procedures, age 60±11 years, 66 males, LA diameter 34±5 mm). Rapid atrial pacing up to 170 ms was performed to induce
    AF
    or AT after EEPVI. Activation mapping of induced AT was performed using EnSite NavX. Results: EEPVI was completed in all patients. After EEPVI,
    AF
    was induced in 13 patients and AT was induced in 31 patients (34 AT). Atrial activation sequences of 27 AT were evaluated. In the 27 AT,
    22
    (81%) were macro-reentrant AT and 5 (19%) were focal AT. In the
    22
    macro-reentrant AT, 11 were peri-mitral AT with a cycle length (CL) of 200±25 ms, 5 were roof-dependent AT (CL 202±56 ms,
    3
    around left PV and 2 around right PV) and
    6
    were atrial flutter around tricuspid annulus (CL 212±
    22
    ms). Conclusions: Majority of AT induced by atrial pacing after the completion of EEPVI are macro-reentrant AT. Three reentrant circuits, i.
    e
    ., around mitral valve, tricuspid valve and pulmonary vein, relate with maintenance of the macro-reentrant AT.
  • Daisuke SATO, Yasuhiro ANDO
    Journal of Oleo Science
    2002年 51 巻 8 号 563-567
    発行日: 2002年
    公開日: 2002/09/26
    ジャーナル フリー
    Fatty acid compositions of neutral lipids (NL) and polar lipids (PL) have been analyzed for a unique sample of Ophiuroidea (brittle star) Ophiura sarsi containing novel nonmethylene-interruped (NMI) fatty acids,
    7E
    ,
    13E
    -icosadienoic (20:2),
    7E
    ,
    13E
    ,17Z-icosatrienoic (20:
    3
    ),
    9E
    ,
    15E
    ,19Z-docosatrienoic (
    22
    :
    3
    ), and 4Z,
    9E
    ,
    15E
    ,19Z-docosatetraenoic (
    22
    :4) acids. All of the NMI fatty acids were concentrated in NL (mainly triacylglycerols) rather than in PL (mainly phosphatidylcholines and phosphatidylethanolamines). On the other hand,
    6
    ,
    9
    ,12,15,18,21-tetracosahexaenoic acid (24:
    6
    n-
    3
    ) usually observed in Ophiuroidea lipids was found in PL at higher concentration than in NL. Biological property and origin of the novel NMI fatty acids appear to be different from those of 24:
    6
    n-
    3
    .
  • Masuko Kobori, Mitsuru Yoshida, Mayumi Ohnishi-Kameyama, Toshiyuki Takei, Hiroshi Shinmoto
    Biological and Pharmaceutical Bulletin
    2006年 29 巻 4 号 755-759
    発行日: 2006年
    公開日: 2006/04/01
    ジャーナル フリー
    We purified a sterol with antitumor activity from the edible mushroom Sarcodon aspratus (BERK.) S. ITO and identified it as 5α,8α-epidioxy-
    22E
    -ergosta-
    6
    ,
    9
    (11),
    22
    -trien-
    -ol (
    9
    ,11-dehydroergosterol peroxide (
    9
    (11)-DHEP)). Purified
    9
    (11)-DHEP was a more effective inhibitor of HL60 leukemia cell growth and stronger apoptosis-inducer than 5α,8α-epidioxy-
    22E
    -ergosta-
    6
    ,
    22
    -dien-
    -ol (ergosterol peroxide (EP)) that we had previously identified as an apoptosis inducer. Moreover,
    9
    (11)-DHEP selectively suppressed the growth of HT29 human colon adenocarcinoma cells but not WI38 normal human fibroblasts. After 5 d incubation of HT29 with 7 μM
    9
    (11)-DHEP, the number of S phase cells decreased from 23 to 15% of total diploid cells and 17% became hypodiploid. Expression of the cyclin-dependent kinase inhibitor 1A (p21, WAF1, Cip1) (CDKN1A), which has been shown to cause cell cycle arrest and apoptosis in HT29 cells, was induced by
    9
    (11)-DHEP. These results suggest that
    9
    (11)-DHEP inhibits HT29 cell growth by inducing CDKN1A expression, thus causing cell cycle arrest and apoptosis.
  • Yasunori YAOITA, Keiko AMEMIYA, Hiroyuki OHNUMA, Katsuyuki FURUMURA, Akihiro MASAKI, Toshihiko MATSUKI, Masao KIKUCHI
    Chemical and Pharmaceutical Bulletin
    1998年 46 巻 6 号 944-950
    発行日: 1998/06/15
    公開日: 2008/03/31
    ジャーナル フリー
    Eight new sterols, 5α, 8α-epidioxy-(
    22
    E
    , 24R)-23-methylergosta-
    6
    ,
    22
    -dien-
    -ol (1),
    , 5α,
    -trihydroxy-(
    22
    E
    , 24R)-23-methylergosta-7,
    22
    -dien-
    6
    -one (2),
    , 5α,
    -trihydroxy-(24S)-ergost-7-en-
    6
    -one (
    3
    ),
    , 5α,
    , 14α-tetrahydroxy-(
    22
    E
    , 24R)-ergosta-7,
    22
    -dien-
    6
    -one (4), (
    22
    E
    , 24R)-ergosta-7,
    22
    -diene-
    , 5α,
    ,
    -tetrol (5), 5α,
    -epidioxy-
    -hydroxy-(
    22
    E
    , 24R)-ergosta-7,
    22
    -dien-
    6
    -one (
    6
    ), 5α,
    -epidioxy-
    -hydroxy-(24S)-ergost-7-en-
    6
    -one (7) and 5α,
    -epoxy-(
    22
    E
    , 24R)-ergosta-8,
    22
    -diene-
    , 7β, 14α-triol (8), have been isolated from five edible mushrooms, Lentinus edodes, Flammulina velutipes, Hypsizigus marmoreus, Pleurotus ostreatus and Pholiota nameko together with fifteen known ones (
    9
    -23), of which two (16 and 17) are reported for the first time from a fungal source. The structures of these new compounds were elucidated on the basis of their spectral data.
  • Yasunori YAOITA, Makiko ENDO, Yoshino TANI, Kaori MACHIDA, Keiko AMEMIYA, Katsuyuki FURUMURA, Masao KIKUCHI
    Chemical and Pharmaceutical Bulletin
    1999年 47 巻 6 号 847-851
    発行日: 1999/06/15
    公開日: 2008/03/31
    ジャーナル フリー
    Six new sterols, 5α,
    ;8α,
    -diepoxy-(
    22
    E
    , 24R)-ergost-
    22
    -ene-
    , 7α-diol (1), 5α,
    ;8α,
    -diepoxy-(
    22
    E
    , 24R)-ergost-
    22
    -ene-
    , 7β-diol (2), 5α,
    -epoxy-(
    22
    E
    , 24R)-ergosta-8,
    22
    -diene-
    , 7β-diol (
    3
    ), (
    22
    E
    , 24R)-23-methylergosta-7,
    22
    -diene-
    , 5α,
    -triol (4), (
    22
    E
    , 24R)-23-methulergosta-7,
    22
    -diene-
    , 5α,
    ,
    -tetrol (5) and (24S)-ergost-7-ene-
    , 5α,
    ,
    -tetrol (
    6
    ), have been isolated from seven mushrooms, Amanita pantherina, Amantia virgineoides, Lactarius piperatus, Lyophyllum shimeji, Tricholoma portentosum, Hypsizigus marmoreus and Lentinula edodes together with eighteen known ones (7-24). The structures of these new compounds were elucidated on the basis of their spectral data.
  • Jozsef RÁTKY, Helmut TORNER, Istvan EGERSZEGI, Falk SCHNEIDER, Peter SARLOS, Noboru MANABE, Klaus-Peter BRÜSSOW
    Journal of Reproduction and Development
    2005年 51 巻 1 号 109-115
    発行日: 2005年
    公開日: 2005/03/07
    ジャーナル フリー
    The aim of the present study was to assess follicular and oocyte development in the same gilts during three phases of their reproductive life [prepuberal gilts (PP;
    6
    .0 months of age), puberal gilts (P;
    9
    .5 months of age) and primiparous sows (S)]. Follicular development was stimulated by the injection of 1,000 IU of equine chorionic gonadotropin (eCG) followed by 500 IU of human chorionic gonadotropin (hCG) 72 h later. Cumulus-oocyte-complexes (COCs) were recovered by endoscopic ovum pick up/aspiration from preovulatory follicles of the left ovary, and the follicular fluid (FF) from the right ovary was collected 34 h after the hCG treatment by endoscopy. Altogether, 19 pigs were used in the PP and P trials and 12 in the S trial. From the left ovaries, 168, 190 and
    82
    follicles were aspirated and 106, 125 and 42 COCs, respectively, were recovered (recovery rate 61 ± 27, 63 ± 21 and 53 ±
    22
    %, respectively). The mean number of follicles was greater in the P phase than in the PP phase (19.7 ±
    6
    .8 vs. 15.7 ±
    6
    .8; p=0.06) and S phases (14.2 ± 4.0; p<0.05). More uniform oocytes with an expanded cumulus were aspirated in the P and PP phases than in the S phase (90 and 78 vs. 46%; p<0.05). Furthermore, the meiotic configuration in oocytes (T I/M II stage) differed between the three phases (56 and 62 vs. 0%; p<0.05). Progesterone (P4) levels in FF decreased from 590.0 ± 333.
    6
    (PP) to 249.1 ± 72.
    6
    (P) and 161.4 ± 75.2 ng/ml (S) (p<0.05). Estradiol-17β (
    E
    2) levels differed between PP and P gilts and S sows (
    9
    .
    3
    ± 2.
    9
    , 21.
    9
    ± 10.
    6
    and
    94
    .0 ± 15.
    9
    pg/ml, respectively; p< 0.05), and the P4/
    E
    2 ratio was 72, 15 and 5, respectively. These results indicate differences in follicular and oocyte development between the reproductive phases investigated. Puberal gilts should preferably be used in IVF and breeding programs. The lower reproductive potential of primiparous sows must be taken into consideration in breeding. Any prediction of lifetime performance based on individual ovarian reactions of prepuberal gilts is unreliable.
  • Hideharu Okamatsu, Takahiro Ohara, Hideaki Kanzaki, Ikutaro Nakajima, Koji Miyamoto, Hideo Okamura, Takashi Noda, Takeshi Aiba, Kengo Kusano, Shiro Kamakura, Wataru Shimizu, Kazuhiro Satomi
    Circulation Journal
    2015年 79 巻 2 号 419-424
    発行日: 2015/01/23
    公開日: 2015/01/23
    [早期公開] 公開日: 2014/11/28
    ジャーナル フリー HTML
    Background:The relationship between outcome of radiofrequency catheter ablation (RFCA) for atrial fibrillation (
    AF
    ) and the severity of left ventricular (LV) diastolic dysfunction in patients with hypertrophic cardiomyopathy (HCM) remains unknown.Methods and Results:Twenty-two HCM patients (12 female, aged 65±11 years) with paroxysmal (n=5; 23%) or persistent (n=17; 77%)
    AF
    were enrolled. LV diastolic function was evaluated according to the ratio of the mitral inflow early filling velocity to the velocity of the early medial mitral annular ascent (
    E
    /
    e
    ’) measured on pulsed wave and tissue Doppler assessments in all patients. Pulmonary vein isolation was performed in all patients. A second procedure was performed in
    3
    patients. During a follow-up of 21±12 months, sinus rhythm was maintained in 13 of
    22
    patients (59%).
    E
    /
    e
    ’ was significantly higher in the patients with
    AF
    recurrence than in those without (18±7 vs. 11±
    3
    ; P<0.01). On Kaplan-Meier analysis the prevalence of
    AF
    recurrence was significantly higher in patients with
    E
    /
    e
    ’ ≥15 (n=
    6
    ) than in those with
    E
    /
    e
    ’ <15 (n=16; P<0.01). On multivariate Cox regression analysis the only significant and independent predictor for
    AF
    recurrence was
    E
    /
    e
    ’ (hazard ratio, 1.16; 95% confidence interval: 1.01–1.37, P=0.03).Conclusions:LV diastolic dysfunction evaluated using
    E
    /
    e
    ’ was associated with difficulty of rhythm control after RFCA in patients with HCM and
    AF
    . (Circ J 2015; 79: 419–424)
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