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  • Joey
    B
    . Tanney, Leonard J. Hutchison
    Mycoscience
    2012年 53 巻 1 号 31-35
    発行日: 2012年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–

    1
    ,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

  • Keishi Hata, Fuyuki Sugawara, Naganori Ohisa, Saori Takahashi, Kazuyuki Hori
    Biological and Pharmaceutical Bulletin
    2002年 25 巻 8 号 1040-1044
    発行日: 2002年
    公開日: 2002/08/01
    ジャーナル フリー
    We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC
    3
    T
    3
    -
    E
    1
    . Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC
    3
    T
    3
    -
    E
    1
    cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (
    22E
    ,24R)-ergosta-
    7
    ,
    22
    -diene-
    ,
    ,
    -triol (
    1
    ).
    1
    induced ALP activities of MC
    3
    T
    3
    -
    E
    1
    cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC
    3
    T
    3
    -
    E
    1
    cells by
    1
    , ergosterol (2), ergocalciferol (
    3
    ), cholesta-
    ,
    ,
    -triol (4),
    7
    -dehydrocholesterol (
    5
    ) and cholecalciferol (
    6
    ) were tested. The enzyme activities of MC
    3
    T
    3
    -
    E
    1
    cells were increased
    3
    .0-fold by 10 μM
    1
    and 2.4-fold by 10 μM 4. However, 2,
    3
    ,
    5
    and
    6
    did not induce MC
    3
    T
    3
    -
    E
    1
    cell ALP activity at 0.
    1
    —10 μM. These results suggested that the OH groups at C-
    5
    and/or C-
    6
    of
    1
    and 4 played an important role in their differentiation-inducing activities on MC
    3
    T
    3
    -
    E
    1
    cells. Furthermore,
    1
    suppressed induction of MC
    3
    T
    3
    -
    E
    1
    cell apoptosis by serum starvation.
  • 小田切 孝人, 田代 眞人
    ウイルス
    2013年 63 巻 2 号 233-240
    発行日: 2013/12/25
    公開日: 2014/10/31
    ジャーナル フリー
  • Suguru TAKATSUTO, Kiyomi KOBAYASHI, Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE
    Agricultural and Biological Chemistry
    1988年 52 巻 12 号 3217-3218
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Özkan ASLANTAŞ, Ebru Şebnem YILMAZ
    Journal of Veterinary Medical Science
    2017年 79 巻 6 号 1024-1030
    発行日: 2017年
    公開日: 2017/06/16
    [早期公開] 公開日: 2017/04/27
    ジャーナル フリー

    This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

    E
    . coli isolates were detected in
    95
    (
    22
    .2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: blaCTX-M-15 (62), blaTEM-
    1b
    (42), blaCMY-2 (
    22
    ), blaCTX-M-
    3
    (16), blaCTX-M-
    1
    (15), blaOXA-
    1
    (
    9
    ) and blaSHV-12 (
    3
    ) alone or in combination. The most frequently encountered phylogenetic group was group
    A1
    (35.8%), followed by group D2 (
    22
    .
    1
    %),
    B
    1
    (15.8%),
    D1
    (
    9
    .
    5
    %), A0 (
    7
    .4%),
    B22
    (
    5
    .
    3
    %) and
    B23
    (4.2%), respectively. PMQR genes, aac(
    6
    ’)-Ib-cr,
    qnrS1
    and qnrB10 were detected in 25.
    3
    , 10.
    5
    and
    1
    .
    1
    % of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.0% for tobramycin to 56.8% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE,
    f17a
    -A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing
    E
    . coli
    clone O25
    b
    :ST131/
    B
    2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing
    E
    . coli
    isolates among dogs in Turkey.

  • George W. Lee
    燃料協会誌
    1973年 52 巻 3 号 171-177
    発行日: 1973/03/20
    公開日: 2010/06/28
    ジャーナル フリー
  • Tsuyoshi WATANABE, Hiroki KURIYAMA, Tokuo FURUSE, Kiyomi KOBAYASHI, Suguru TAKATSUTO
    Agricultural and Biological Chemistry
    1988年 52 巻 8 号 2117-2118
    発行日: 1988年
    公開日: 2006/04/05
    ジャーナル フリー
  • Grover Loening
    日本航空学会誌
    1963年 11 巻 119 号 373-380
    発行日: 1963年
    公開日: 2009/05/25
    ジャーナル フリー
  • Hin-chung Wong, Chia-Yun Ho, Li-Ping Kuo, Tien-Kuei Wang, Chi-Lung Lee, Daniel Yang-Chih Shih
    MICROBIOLOGY and IMMUNOLOGY
    1999年 43 巻 7 号 631-636
    発行日: 1999年
    公開日: 2008/03/17
    ジャーナル フリー
    Vibrio parahaemolyticus is a prevalent food-borne pathogen in Taiwan, Japan and other Asian countries. This work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. Genomic DNA was fragmented by HindIII digestion and hybridized with cDNA probe for Escherichia coli 16S and 23S RNA genes. A total of 121 isolates obtained from outbreaks during 1992 and 1994 in Taiwan were characterized by this ribotyping method. Four to seventeen restricted fragments were visualized in these isolates. After hierarchical cluster analysis, these isolates were grouped into thirty different ribotypes. In addition, A
    3
    , A
    7
    ,
    E
    3
    and
    F
    1
    were the major ribotypes, consisting of
    22
    .
    3
    , 13.2,
    9
    .
    1
    , and 8.
    3
    % of the isolates, respectively. A,
    E
    ,
    F
    , G and
    B
    were the major groups, consisting of 46.2, 14.0,
    9
    .
    1
    ,
    6
    .
    7
    , and
    6
    .
    7
    % of the isolates, respectively. The discriminatory ability of this ribotyping method, as determined by Simpson's index of diversity, was 0.
    93
    , which closely resembled that of a previously reported pulsed-field gel electrophoresis method.
  • 末次 康将
    マリンエンジニアリング
    2021年 56 巻 5 号 680-687
    発行日: 2021/09/01
    公開日: 2021/10/12
    ジャーナル フリー
  • 佐藤 敬之輔
    デザイン学研究
    1970年 1970 巻 11 号 45-55
    発行日: 1970/03/30
    公開日: 2017/07/25
    ジャーナル フリー
    [
    1
    ] The purpose of this reserch is to make clear what type of design can give good effects to win the high readership score. At the begining I had to know what factors decide the readership score, and how to change the quality of the desing into numerical value. I have used the method of multi-dimentional analysis with the help of a computer, and got the estimation formula, with
    7
    kinds of factors, as follows ; A^^〜=
    B
    +C+D+K+aE+bF+cG. A^^〜=estimated values of the readership score.
    1
    )
    B
    , C, D, K : these four factors are given as category values (so we call them); each factor is classified into several categories.
    B
    =week day, classified into
    7
    categories. C=pages of a newspaper, which are characterized by the kinds of articles, news, and the other reading matters, classified into
    22
    categories. D=kinds of goods and trades of the advertizement, classified into 14 catedories. K=constants, given to each 13 surveys. 2)
    E
    ,
    F
    , G : these factors are given as numerical values. G=area of advertizement, measured by the unit of column in the whole page length, being classified into
    6
    kinds of area (2.
    5
    ,
    3
    .
    5
    ,
    5
    ,
    7
    , 10, 15) and 15 column means a whole page, about 2,000cm^2.
    E
    ,
    F
    are the marks obtained in the design of advertizements.
    E
    =sum of the marks obtained in each design element on the advertizement.
    F
    =the marks obtained on the whole effect of the design.
    3
    ) a,
    b
    , c : coefficients Using the above formula with the category values and the coefficients, we can obtain the naked design effect from the actual value A of readership score: aE+bF=A-(
    B
    +C+D+K+cG). In this research, I used
    1
    ,829 data of readership score from 1960 to 1968, being obtained by 13 survevs, spring and autumn twice a year. The sample of size each survey was
    3
    ,541, 2,251, or 200 in the other 11 surveys. Multiple correlation coefficient between the estimated value A^^〜 and the actual value A is 0.951. The table 2^* shows the contribution indexes of each factor by
    3
    kinds of expression-(
    1
    ) Range : the absolute difference between the maximum and the minimum category values, (2) Standard deviations of the category values, (
    3
    ) Partial correlation coefficient : the relationship between the actual values A and each factors. *see the table 2 in the thesis in Japanese. [2] The important point was in the determination of the values of
    E
    ,
    F
    .
    E
    is the sum of the marks obtained in each design element, classified into four kinds :
    E
    =
    E
    _
    1
    +
    E
    _2+
    E
    _
    3
    +
    E
    _4. design elements [table]
    E
    _
    1
    ,
    E
    _2,
    E
    _
    3
    ,
    E
    _4, these values have the
    5
    grades, as 0,
    1
    , 2,
    3
    , 4 and 0 is given to the design that has no attractive effect or no applicable element. And
    E
    obtain the marks of 0 to
    9
    grades as the sum of them. The values of
    F
    have also
    5
    grades, 0 to 4. The principles to determine the values of
    E
    ,
    F
    are as follows : a) The marks obtained of
    E
    ,
    F
    must be the relative values among the each survey, and at the same time, they must have constancy within the same survey-the same elements of design must win the same marks obtained.
    b
    ) They must be determined as to win the highest multiple correlation coefficient, when they are put into the estimation formula. c) They must be reasonable. In order to justify them, we must carry many researches on the actual condition. The frequency of accurence of each grade of
    E
    ,
    F
    values, as the table
    5
    -4, and
    5
    -
    5
    in the thesis in Japanese.
  • A.M. BRITTO, C. SAVVIDOU, M.J. GUNN, J.R. BOOKER
    土質工学会論文報告集
    1992年 32 巻 1 号 13-25
    発行日: 1992/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    One method currently being considered for the disposal of high level radio-active waste is burial in the seabed. When a hot object is buried in soil, the temperature of the soil adjacent to the object is raised and there is transient heat flow away from the object. The rise in the temperature of the soil causes excess pore pressures to be generated and transient pore water diffusion also takes place. A finite element formulation of the coupled heat flow and consolidation problem is presented. The computer implementation of this formulation is found to compare favourably with analytical solutions based on the same physical principles.
  • Takaaki ISHIZUKA, Yasunori YAOITA, Masao KIKUCHI
    Chemical and Pharmaceutical Bulletin
    1997年 45 巻 11 号 1756-1760
    発行日: 1997/11/15
    公開日: 2008/03/31
    ジャーナル フリー
    Four new sterols,
    ,
    -epoxy-(
    22
    E
    , 24R)-ergosta-8(14),
    22
    -diene-
    ,
    -diol (
    1
    ), (
    22
    E
    , 24R)-ergosta-8,
    22
    -diene-
    ,
    ,
    ,
    -tetrol (2), (
    22
    E
    , 24R)-ergosta-
    7
    ,
    9
    (11),
    22
    -triene-
    ,
    ,
    -triol (
    3
    ) and
    ,
    ,
    -trihydroxy-(
    22
    E
    , 24R)-ergost-
    22
    -en-
    7
    -one (4), have been isolated from the fruit bodies of Grifola frondosa (FR.) S.
    F
    . GRAY (Polyporaceae)together with fourteen known ones (
    5
    -18), of which two (
    5
    and
    6
    ) are reported for the first time from a natural source. The structures of these compounds were elucidated on the basis of spectral data.
  • 小林 優, 家坂 貴子, 中野 江身子, 平山 耕一郎
    天然有機化合物討論会講演要旨集
    1989年 31 巻 71/P2-1
    発行日: 1989/09/17
    公開日: 2017/08/18
    会議録・要旨集 フリー
    Six new cembranoids sarcophytol P (
    3
    ), R (4), S (
    5
    ), K (8),
    F
    (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P (
    3
    ) was shown to be the 20-hydroxy derivative of the major component sarcophytol A (
    1
    ), and afforded the cyclization product
    6
    in CHCl_
    3
    at room temperature, in a same way as in
    1
    . Sarcophytols R (4) and S (
    5
    ) were correlated to
    1
    , by conversion of its
    7
    R,8R and
    7
    S,8S epoxide derivatives. Sarcophytol K(8) was a 13, 14-dihydroxycembranoid having a
    1
    E
    ,
    3
    Z-diene moiety. The absolute configuration of 8 and its
    1
    Z,
    3
    E
    - and
    1
    Z,
    3
    Z-isomers sarcophytol
    B
    (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols
    F
    (11) and T (13) were
    1
    E
    - and
    1
    E
    ,
    3
    Z-isomers of
    1
    . Compound 11 showed characteristic broadening of the ^
    1
    H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols
    F
    (11,
    1
    E
    ,
    3
    E
    ), N (15,
    1
    Z,
    3
    Z) and T (13,
    1
    E
    ,
    3
    Z), spontaneous autoxidation-cyclization, in CHCl_
    3
    ,was examined, in order to compare the stereochemical course of the reaction with that of
    1
    (
    1
    Z,
    3
    E
    ), which affords trans-fused bicyclo[
    9
    .
    3
    .0]tetradecene systems. The
    1
    E
    ,
    3
    Z-isomer 13 gave the same cyclization product 18, as in the reaction of
    1
    , even though it is isomeric at C-
    1
    ,
    3
    . The
    1
    Z,
    3
    Z isomer 15 gave 19, in consequence of the reversed geometry at C-
    3
    of 15. The
    1
    E
    ,
    3
    E
    -isomer (11) gave the bicyclic product
    22
    , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and
    22
    , giving enantiomeric ketones 23 and 24 respectively. The
    1
    (14)-epoxide 26 was shown to be the immediate precursor of
    22
    , and acounted for the inversion of the geometry at C-
    1
    of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from
    1
    . The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and
    22
    , was controlled by the chirality of the epoxy rings. The C-14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene C (30), the parent hydrocarbon of
    1
    , also reacted in CHCl_
    3
    giving the bicyclic product 32.
  • T BAKER, C. RANGACHARYULU
    日本物理学会講演概要集
    1999年 54.2.1 巻 23pSM-6
    発行日: 1999/09/13
    公開日: 2018/03/04
    会議録・要旨集 フリー
  • Shingo Nakamura, Tadahiro Hasegawa, Tsubasa Hiraoka, Yoshinori Ochiai, Shin’ichi Yuta
    Journal of Robotics and Mechatronics
    2018年 30 巻 4 号 540-551
    発行日: 2018/08/20
    公開日: 2018/08/20
    ジャーナル オープンアクセス

    The Tsukuba Challenge is a competition, in which autonomous mobile robots run on a route set on a public road under a real environment. Their task includes not only simple running but also finding multiple specific persons at the same time. This study proposes a method that would realize person searching. While many person-searching algorithms use a laser sensor and a camera in combination, our method only uses an omnidirectional camera. The search target is detected using a convolutional neural network (CNN) that performs a classification of the search target. Training a CNN requires a great amount of data for which pseudo images created by composition are used. Our method is implemented in an autonomous mobile robot, and its performance has been verified in the Tsukuba Challenge 2017.

  • Zhe Fang, Su Yang Jeong, Hyun Ah Jung, Jae Sue Choi, Byung Sun Min, Mi Hee Woo
    Chemical and Pharmaceutical Bulletin
    2010年 58 巻 9 号 1236-1239
    発行日: 2010/09/01
    公開日: 2010/09/01
    ジャーナル フリー
    Activity-directed isolation of the ethyl acetate, methylene chloride and n-hexane fractions of Gloiopeltis furcata resulted in the isolation of 18 compounds. Their structures were elucidated as 2-(
    3
    -hydroxy-
    5
    -oxotetrahydrofuran-
    3
    -yl)acetic acid (
    1
    ), glutaric acid (2), succinic acid (
    3
    ), nicotinic acid (4), (
    E
    )-4-hydroxyhex-2-enoic acid (
    5
    ), cholesterol (
    6
    ),
    7
    -hydroxycholesterol (
    7
    ), uridine (8), glycerol (
    9
    ),
    5
    -(hydroxymethyl)-2-methoxybenzene-
    1
    ,
    3
    -diol (10), (
    5E
    ,
    7E
    )-
    9
    -oxodeca-
    5
    ,
    7
    -dienoic acid (11), (Z)-
    3
    -ethylidene-4-methylpyrrolidine-2,
    5
    -dione (12), dehydrovomifoliol (13), loliolide (14), cholesteryl stearate (15), palmitic acid (16), cis-
    5
    ,8,11,14,17-eicosapentaenoic acid (17) and α-linolenic acid (18) on the basis of spectroscopic and chemical evidences. Their anticholinesterase and antioxidant activities were evaluated via inhibitory activities on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as well as scavenging activities on
    1
    ,
    1
    -diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO). All isolated compounds (
    1
    18) exhibited moderate AChE inhibitory activities with IC50 values ranging from
    1
    .14—12.50 μg/ml, whereas
    1
    ,
    7
    ,
    9
    , 17, and 18 showed mild BChE inhibitory activities with IC50 values ranging from
    5
    .57—15.89 μg/ml. Although most of the compounds isolated were lacking the scavenging activity on DPPH radical and ONOO,
    5
    and 10 showed good DPPH radical scavenging activity, and
    5
    , 10, and 16 showed potent ONOO scavenging activity.
  • 上野 千草, 松本 第佑, 丸山 記美雄
    土木学会論文集
    E
    1
    (舗装工学)

    2023年 78 巻 2 号 I_92-I_99
    発行日: 2023年
    公開日: 2023/02/22
    ジャーナル フリー

     コンクリート舗装はアスファルト舗装よりもわだち掘れやひび割れに対する耐久性が高く,舗装の長寿命化が期待される.しかしながら,寒冷地では凍上対策を確実に実施されない場合,早期に破損が生じることが知られている.本研究では,既設アスファルト舗装をコンクリート舗装へ打ち換える場合の凍上対策として,XPS(押出法ポリスチレンフォーム断熱材)を用いた断熱工法の適用性を試験施工により検討した.断熱材の保護および路盤の品質確保のため必要となる路盤厚さの検討,断熱材の耐力照査,および熱伝導解析による断熱効果のシュミレーション結果に基づいて試験施工を実施した結果,良好な施工が可能であること,路床の支持力低下が懸念される融解期においても通常期と同等の支持力が得られること,期待される断熱効果が得られることが明らかとなった.

  • 岡本 信也, 齋藤 佑太, 山本 貴士, 前川 敬彦
    土木学会論文集
    E
    1
    (舗装工学)

    2023年 78 巻 2 号 I_84-I_91
    発行日: 2023年
    公開日: 2023/02/22
    ジャーナル フリー

     ある道路トンネル内に使われているプレストレストコンクリート舗装(以下,PC舗装)において,縦断方向及び横断方向のひび割れが確認された.現地調査の結果,大きなひび割れが発生している箇所では,PC鋼材の破断や,たわみが大きな箇所では,PC舗装版と路盤の間に空洞の確認もされた.

     そこで,それら2つの要因が,PC舗装のたわみにどのように影響を及ぼしているかを把握するために

    3
    次元非線形FEMによる解析的検証を実施した.解析の結果,空洞の大きさの方が,プレストレス減少よりも,たわみに与える影響が大きいことが分かった.加えて,空洞が生じると,PC舗装のみで荷重に抵抗する梁形式に近い耐荷機構に変化すること,さらに,舗装表面に負曲げが生じる可能性があることが分かった.

  • 呉 悦樵, 瀬尾 彰
    土木学会論文集
    E
    1
    (舗装工学)

    2023年 78 巻 2 号 I_75-I_83
    発行日: 2023年
    公開日: 2023/02/22
    ジャーナル フリー

     近年,空港,高速道路ならびに国道の開削調査等により,舗装の損傷における水の影響が明らかになってきた.舗装の長寿命化を実現するには,水が介在することで生じる損傷の過程を正しく理解することが大きな意味を持つ.そのためには,室内評価において,供用中の道路で実際に観測されるような損傷を再現でき,加えて耐水性能の異なるアスファルト混合物に対して,その性能差を有意に比較検討できる評価方法を獲得することが重要と考える.本検討では,既往研究で究明したアスファルトの骨材からの剥離メカニズムを踏まえ,国内・海外の試験法を参考に新たに開発した,改質アスファルト混合物の新たな耐水性評価法を提案し,さらに試験精度を高める手法も検討した.

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