The major floral pigment of petunia, which is either a cyanidin-series (cyanidin and peonidin derivatives) or a delphinidin-series anthocyanin (delphinidin, petunidin and malvidin derivatives), is determined by the function of a flavonoid-3′,5′-hydroxylase that is encoded by two independent loci,
Hf1 and
Hf2. At the
Hf2 locus, the recessive allele
hf2-1 had a deletion of about 200 base pairs in the first intron and eight missense mutations by base substitution in the exons. The transcript level of
hf2-1 was markedly lower than that of
Hf2. We developed PCR-based markers to determine genotypes of the
Hf1 and
Hf2 loci. This technique allows amplified fragments from the
Hf1,
hf1-2 and
hf1-3 alleles, as well as from the
Hf2 and
hf2-1 alleles, to be distinguished from one another. These PCR-based analyses showed no discrepancy between the
Hf1/
Hf2 loci genotypes and the major floral anthocyanidin phenotypes in the 129 commercial petunias we examined. Another reported recessive mutant allele at the
Hf1 locus, caused by the insertion of an
Spm-like transposable element in the second exon (
hf1-1), was not found in the commercial petunias.
抄録全体を表示