1951 Volume 38 Issue 2 Pages 199-212
1. Two kg. of lyophilized horse blood stroma was prepared and extracted with organic solvents.
2. From the ether-insoluble lipid fraction, palmityl-sphingoniyelin and a glycolipid, which is described under the name of hematoside, were isolated.
3. The cleavage products from both lipids were studied in detail.
ADDENDUM: I . After this paper was submitted, Prof. Dr. E. Klenk had the kindness to send us his detailed papers. According to the report (21), he already noticed that neuraminic acid in paper (19) possessed a methoxyl group. He used, thereafter, the term neuraminic acid, C10H19O9N, for the non-methoxyl substance which corresponds our prehemataminic acid. Whereas he prefer for the methoxyl-containing acid C11H21O9N to C10H19O8N, we take the latter for our hemataminic acid.
2. Though the structural study of hematoside is being continued, it is justified to state that the amino group of prehemataminic acid in the original undegraded lipid is probably substituted by an acyl group, since no amino nitrogen is found in hematoside by Van Slyke's method.