1963 Volume 53 Issue 1 Pages 28-36
Globoside, the main mucolipid of human erythrocytes, was purified by repeated silicic acid column chromatography. Its component fatty acids were mainly C22-C24 group, in which nervonic acid was predominating. By mild acid hydrolysis and subsequent charcoal chromatography of liberated oligosaccharide mixture, N-acetylgalactosaminoyl (1→6) galactose was isolated. After complete methylation and subsequent methanolysis, globoside yielded several methylated hexoses, which were identified by gas-liquid chromatography. These results lead to a constitutional formula of N-acetyl galactosaminoyl (1→6) galactosyl (1→4)galactosyl (1→4) glucosyl-ceramide.
The authors wish to express their gratitude to Assist. Prof. Z. Yosizawa and Assist. Prof. M. Tomoda for carbohydrate samples, to Dr. M. Kitano of Nippon Blood Bank for the supply of human erythrocytes. They are also indebted to their colleagues in this laboratory for discussion and technical assistances. The expense of this study was aided by the Scientific Fund furnished by the Ministry of Education for which they wish to thank.