2010 Volume 75 Issue 3 Pages 319-325
Sex-linked genetic markers of Silene latifolia were mapped to the sex chromosomes with the aim of analyzing their evolutionary origins. SlAP3X/Y has been particularly uncertain for years. Its X-counterpart has not been identified, and very few Y-deletion mutants delete SlAP3Y. In this study, we verified the sex chromosome-linkage of SlAP3X and SlAP3Y through cloning, sequencing, and fluorescent in situ hybridization (FISH). SlAP3X, spanning a region of 1.6 kb, consists of 6 exons and 5 introns. SlAP3Y has 7 exons and 6 introns, the second intron being particularly large (24.4 kb). SlAP3Y spans 31 kb in total. Their Harr-plot analysis and the chromosome positions indicated by FISH using their own probes support the hypothesis that the translocation of SlAP3X/Y has occurred on either chromosome.
