Studies on the Insoluble States of Anthocyanin in Rose Petals
II. Histochemical observation on its basal substance
1976 Volume 41 Issue 3-4 Pages 487-492
Details
1976 Volume 41 Issue 3-4 Pages 487-492
The present paper dealt with some histochemical observation on the basal substance of the insoluble state of anthocyanin appearing in the upper epidermal cells of petals, using a black rose cultivar, Charles Mallerin.
The results obtained are summarized below.
1. The satisfactory fixation and decoloration of the insoluble state of anthocyanin were accomplished by Müller's solution, 10% neutral buffered formalin and Scott's solution, leaving a massive structure.
2. The insoluble state of anthocyanin treated with the solutions above mentioned was stained orange with safranin, and red with ruthenium red very strongly.
3. These stainings are strongly suggestive that the pectic substance is involved in the basal substance of the insoluble state of anthocyanin.
4. It may be considered that the pectic substance of this massive structure is not calcium salt of pectic acid but protopectin.
5. The insoluble state of anthocyanin in the present material can be interpreted as a special structure of the pectic substance, protopectin, associated anthocyanin.
