Cloning and sequencing of mouse tyrosinase cDNA

Hiroaki YAMAMOTO; Sakae TAKEUCHI; Toshiyuki KUDO; Kozo MAKINO; Atsuo NAKATA; Tomotaka SHINODA; Takuji TAKEUCHI

doi:10.1266/jjg.62.271

Hiroaki YAMAMOTO, Sakae TAKEUCHI, Toshiyuki KUDO, Kozo MAKINO, Atsuo NAKATA, Tomotaka SHINODA, Takuji TAKEUCHI

Author information

JOURNAL FREE ACCESS

1987 Volume 62 Issue 3 Pages 271-274

DOI https://doi.org/10.1266/jjg.62.271

Details

Abstract

A cDNA library was constructed from poly (A)+ mRNA from mouse melanocytes and screened using anti-tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. Sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides.

Corresponding author

Register with J-STAGE for free!