Abstract
In order to understand the evolutionary process of self-incompatibility, we must know the genetic variability of the self-incompatibility genes within and between populations. Statistical methods for estimating the effective number of alleles, expected heterozygosity and genetic distance from pollination experiments were developed, which can be applied to both gametophytic and sporophytic self-incompatibility systems. In these methods, bud-pollination, which is necessary for obtaining homozygotes, is not required. Since bud-pollination, which is time-consuming, is not required in the present methods, they might be useful.
