Abstract
A high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) using an ion-exchange (DEAE-form) column shows three components: The principal component corresponds to human mercaptalbumin (HMA); the secondary to nonmercaptalbumin (HNA), having mixed disulfide with cystine (HNA[Cys]), or oxidized glutathione (HNA[Glut]); and the tertiary to HNA, oxidized more highly than mixed disulfide. The purpose of the present study is to clarify the effects of strenuous exercise load on HMA↔HNA; conversion (i.e., dynamic change in redox state) of HSA from elite kendo athletes (n = 30; 20.0±1.1 years old). They participated in an intensive kendo training camp for 5 d. The mean value for the HMA fraction (f−[HMA]) of kendo athletes after camp (62.8±2.4%) was significantly lower than before camp (71.9±3.7%) (p<0.0005). In contrast, the mean value for f−(HNA-1) (i.e., f−[HNA(Cys) and HNA(Glut)]) after camp (34.2±2.1%) was significantly higher than before camp (25.7±3.7%) (p<0.0005). These results suggested that strenuous physical exercise markedly increased the oxidized albumin level in extracellular fluids during the intensive training camp.
