Detection and typing of human papillomavirus DNA in precancerous and cancer lesions of the uterine cervix with scraped samples

Abstract

We have followed up patients with dysplasia to study the relationship between HPV infection as an oncogenic factor and cervical cancer. For this purpose, we did not perform punch biopsy except as the first biopsy for diagnosis. Cervical and vaginal pooled cells were collected to detect HPV DNA by Southern blot hybridization.
Although a plurality of HPV types was detected in CIN lesions, HPV 16 was prevalent in CIN III. HPV 16 was also prevalent in squamous cell carcinoma, whereas HPV 18 was predominant in adenocarcinoma of the uterine cervix.