Involvement of the IL-6/STAT3/Sca-1 system in proliferation of duct cells following duct ligation in the submandibular gland of mice

Nunuk Purwanti; Ahmad Azlina; Mileva R Karabasil; Takahiro Hasegawa; Chenjuan Yao; Tetsuya Akamatsu; Kazuo Hosoi

doi:10.2152/jmi.56.253

Development, Regeneration, and Tissue Engineering of the Glandular Tissue

Involvement of the IL-6/STAT3/Sca-1 system in proliferation of duct cells following duct ligation in the submandibular gland of mice

Nunuk Purwanti, Ahmad Azlina, Mileva R Karabasil, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Kazuo Hosoi

Author information

Nunuk Purwanti
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Department of Basic Dental Science, Faculty of Dentistry, Gadjah Mada University
Ahmad Azlina
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Mileva R Karabasil
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Department of Orthodontics, Faculty of Dentistry, Belgrade University
Takahiro Hasegawa
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Chenjuan Yao
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tetsuya Akamatsu
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kazuo Hosoi
Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School

Keywords: submandibular gland, IL-6, STAT3, duct-ligation

JOURNAL FREE ACCESS

2009 Volume 56 Issue Supplement Pages 253-254

DOI https://doi.org/10.2152/jmi.56.253

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Abstract

Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway. J. Med. Invest. 56 Suppl.: 253-254, December, 2009

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