Abstract
We developed super-resolution microscopy using the fluorescence depletion process. To verify the principle of this microscopy, we measured fluorescence images of micro-beads, i.e the point-spread function (PSF). It was found that the FWHM of PSF becomes smaller than 110 nm. The results show that proposed microscopy can provide a sufficient spatial resolution to overcome the diffraction limit. In microscopy, the Laguerre-Gaussian beam with a zero center singularity plays an important role and determines performance of microscopy. We introduce our proposed technique, including formation about a Laguerre-Gaussian beam tightly focused by a high-NA microscope objective lens.
