Carbon nanoparticles, such as carbon nanotubes and fullerene (C
60), are potential candidates as leading substances in nanotechnological fields, but little is known about their safety. Here we examined
in vivo genotoxicity of C
60, by performing the
Pig-A gene mutation assay in the peripheral blood of male C57BL/6Cr mice. Mice were given single intraperitoneal injection of 3 mg of C
60 particles in 0.5 mL suspension containing 0.1%-Tween80-saline. As a positive control for the
Pig-A gene mutation assay, mice were given a single oral administration of
N-nitroso-
N-ethylurea. At 2 and 8 weeks after treatments, we analyzed CD24-negative and -positive red blood cells in peripheral blood and calculated
Pig-A mutant frequencies. As a result, we detected no significant differences in the mutant frequencies between C
60 treated and non-treated mice, indicating that C
60 is negative for genotoxicity
in vivo in the limited target tissues assessed in this study. For the full assessment, we need comprehensive whole body survey on the genotoxicity of C
60.
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