Abstract
Antigen-induced histamine release from human skin passively sensitized with reaginic serum in vitro was described. Human skin obtained at operation was sliced into 500 μm-thick slices by a hand micro-tome. Skin slices were sensitized by reaginic serum from allergic patients and measurement of histamine released from sensitized skin slices by specific antigen was carried out by bioassay. Evidence is presented confirming that antigen-induced histamine release from passively sensitized skin slices is due to a reaction between antigen and IgE antibody. Several conditions for passive sensitization of human skin and antigen-induced histamine release from sensitized skin in vitro were studied in further experiments.
