Exploring a Method for Inter-module Time Delay Training in a Semi-separated Dissociated Neuronal Network

Abstract

Time-delay stimulation in rat cortical slices is considered to be a good experimental model for studying temporal learning, an intrinsic property of the cortical circuit. However, limited knowledge exists surrounding the mechanism of how stimulation with fixed-interval modifies network dynamics. Here, we aimed to elucidate network structure dependency in temporal learning by examining whether the same stimulation is affective for dissociated cultures. A neuronal network with a modular structure was formed with a micro-fabricated device. While training stimulation was effective for altering firing rate, no clear difference was shown in network dynamics, suggesting that the same stimulation used in a slice culture was insufficient for dissociated networks.