Abstract
Allergen-specific IgE quantitation using IMMULITE 2000 3gAllergy was evaluated. The analytical performance of this analyzer showed good precision results for within-run and between-run reproducibilities, good recovery from high and low sample dilutions, and low limit of detection from 0.048–0.061 IUA/mL. Furthermore, serum samples were analyzed using ImmunoCAP® and IMMULITE 2000 3gAllergy in terms of specificities for 28 allergens. The levels of specific IgE antibodies to all the allergens determined by both assays highly correlated, showing a Spearman rank-correlation coefficient of over 0.7. Overall, in comparison with ImmunoCAP® results, specific IgE antibody levels measured using IMMULITE 2000 3gAllergy were higher for egg white, aspergillus and cat dander, and lower for wheat, peanut and dog dander.
