Anti-influenza Virus Activity of Coix-seed Reactive Derivatives

Abstract

Objective: We examined anti-influenza virus (anti-IFV) activity of Coix-seed Reactive Derivatives (CRD) extract. Methods: H1N1 A/Puerto Rico/8/34 (PR8) strain was infected to Malin Darby Canine Kidney (MDCK) cells. The infected cells were cultured by medium supplemented CRD extract for 24 h, and the supernatants were harvested to analyze virus titer by focus-forming reduction assay. Polyphenols were removed by PVPP treatment, and anti-influenza virus activity was compared with normal CRD extract. Results: CRD extract showed anti-IFV activity. In addition, CRD extract inhibited viral adsorption and replication. The study of CRD extract by PVPP treatment suggested that polyphenols are the main active component. Conclusion: This study revealed that CRD extract has anti-IFV activity against PR8 strain. The mechanism of action was inhibited viral adsorption and replication. It was also suggested that anti-IFV activity of CRD extract is due to polyphenols.