Detection of Gene Mutations Possibly Related to Methanol Tolerance in Gluconobacter frateurii Mutant Gf398

Yuya Arai; Hideaki Koike; Shun Sato; Yuya Sato; Yosuke Iimura; Hidenori Tani; Toshiharu Yakushi; Kazunobu Matsushita; Hiroyuki Fuse; Hiroshi Habe

doi:10.50963/jenvbio.21.1_59

Original paper (short paper)

Detection of Gene Mutations Possibly Related to Methanol Tolerance in Gluconobacter frateurii Mutant Gf398

Yuya Arai, Hideaki Koike, Shun Sato, Yuya Sato, Yosuke Iimura, Hidenori Tani, Toshiharu Yakushi, Kazunobu Matsushita, Hiroyuki Fuse, Hiroshi Habe

Author information

Yuya Arai
College of Systems Engineering and Science, Shibaura Institute of Technology
Hideaki Koike
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Shun Sato
Research Institute for Sustainable Chemistry, AIST
Yuya Sato
Environmental Management Research Institute, AIST
Yosuke Iimura
Environmental Management Research Institute, AIST
Hidenori Tani
Environmental Management Research Institute, AIST
Toshiharu Yakushi
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
Kazunobu Matsushita
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
Hiroyuki Fuse
College of Systems Engineering and Science, Shibaura Institute of Technology
Hiroshi Habe
Environmental Management Research Institute, AIST

Keywords: acetic acid bacteria, biorefinery, Gluconobacter frateurii, glycerol, methanol-tolerance

JOURNAL FREE ACCESS

2021 Volume 21 Issue 1 Pages 59-62

DOI https://doi.org/10.50963/jenvbio.21.1_59

Details

Abstract

Strain Gf398 is an improved methanol-tolerant mutant of Gluconobacter frateurii NBRC 103465. Comparative genome analysis of Gf398 and NBRC 103465 revealed 18 mutated genes as potentially related to the methanol tolerance of Gf398. These mutated genes included enzyme-related, cell membrane-associated, cellular maintenance, and hypothetical protein-encoding genes, as well as one regulatory gene (ompR). The methanol tolerances of a recombinant NBRC 103465 strain expressing mutated ompR and one expressing the wild-type ompR were compared; the mutant showed slightly better cell growth in 2% (v/v) methanol-containing glycerol medium.

Corresponding author

Register with J-STAGE for free!