Dilution Ratio of Tacca leontopetaloides in the Production of Bioethanol with the Inoculation of Indigenous Yeasts Consortium

Abstract

Aims of the research was to determine the best dilution ratio of Tacca leontopetaloides with the combination of indigenous yeasts in producing bioethanol. The research methodology was experimental with Completely Randomized Design (CRD) 3 × 3 factorial pattern, ANOVA used to analyze the data then the significant treatment continued with Tukey test. The treatments was two factors i.e. Tacca leontopetaloides dilution ratio with water (1:2, 1:3, 1:4) with indigenous yeasts combination C. natalensis + C. krusei + H. guilliermondii (1:1:1), C. natalensis + C. krusei (1:1), C. natalensis + H. guilliermondii (1:1) with three times replication. The diluted Tacca leontopetaloides was ferment at room temperatures (25-27 °C) for 48 hours and then tested with chromium dichromate oxidation method to determine the bioethanol contents. The results showed that mixed treatments of dilution ratio with indigenous yeasts combination and single treatments of dilution ratio gave significant effects towards bioethanol contents, while the combination of indigenous yeasts gave non-significant effects. The best treatments showed by the dilution ratio of 1:2 with the combination of C. natalensis and H. guilliermondii (1:1) that resulting in 3.46% of bioethanol.