Low Temperature Storage of Rat 2-cell Embryos by Vitrification

Abstract

Rat 2-cell embryos were preserved successfully in liquid nitrogen by vitrification. Embryos needed to be equilibrated in 90%VS1 in a stepwise manner, while protective agents could be removed directly from the vitrified embryos. The vitrified embryos developed to live youngs at high rates (59-67%) by transferring to pseudopregnant recipients.