To solve the problems in difference of flowering periods among edible Allium species, the pollen of Allium species was stored until pollination. The pollen stored at - 30°Cmaintained higher germinability than that stored at 5°C in all Allium species examined. The results of the pollen viability test, expressed as a pollen germination rate after anthesis, revealed that the pollen should be harvested as soon as anthesis occurs or at latest within 12 hours. In the crossing between A. chinense and A. fistulosum, the optimum conditions for ovary culture of interspecific hybrids were clarified. It was important to initiate ovary culture 5 days after pollination and to culture ovaries on MS medium containing 5 % sucrose at 25 °C. By this ovary culture, the hybird plants between A. chinense and A. fistulosum, two genotypes of A. cepa. A. tuberosum or A. ampeloprasum were obtained and their hybridity was confirmed by DNA analysis.