1955 Volume 44 Issue 8 Pages 767-777
In estimating oxygen consumption and glycolysis of bone marrow by warburg's apparatus, the method of making slices seems to have much influence upon results. When the obstruction imposed on the tissue proves intense, it is hard to obtain a constant results. We, inserting the tissue of bone marrow between two Graefe's messes, and by cutting while smoothly moving these knives on both sides, could succeed in getting thinkness of the slices below 0.5mm.
In a normal rabbit these slices, of bone marrow have always proved greater amount of oxygen consumption than in case of minced one; besides, the lowering rate according to the lapse of time was smaller. As for the floating solution, relatively constant results were obtained with saline phosphate solution, but Ringer-bicarbonate solution possesses greater value than those of saline phosphate solution or Tyrode's solution, showing slightest in their lowering rate in the course of time. The optimum pH in the floating solution seems at pH 7.4.
Subsequently, as for sternal punctate, clinical application would be made possible by making conditions of both puncture as well as suction more steady. Results may need to be judged from all these figures: Xo2, Xo2/K, Qo2, XMN2, XMN2, /K, QMN2, (K=nucleated cells in 1cmm punctate).
If considered as to various kind of diseases in series of microcytic hypochromic anemia, oxygen consumption has indicated approximately normal, or somewhat excessive accompanied with a moderate advance in glycolysis. In case of hypoplastic anemia, the both function lowers remarkably, moreover in the type of blood cell arrest, the lowering ratio proved slight, while proved marked with the type of regeneration disturbance, mixed type or panmyelophtisis. Also, in hypoplastic anemia, Xo2, XMN2, /X show pretty high so that it appears almost antipodinous compared to leukemia. Among various leukemias, the rise in oxygen consumption in case of monocytic leukemia appears remarkably unique.
Thus, we could realize that oxygen consumption and glycolsis in the bone marrow possess very close connection with hematopoietic function, playing an important role in the explanation of ethological physiology of bone marrow as well as clinical differential diagnosis.
