In vitro evaluation of bioresorbability of apatite-fiber scaffolds using an acetic acid buffer as a model of quasi-osteoclastic producting solution

Abstract

We have developed porous apatite-fiber scaffolds (AFSs) for tissue engineering of bone using single-crystal apatite fibers. In order to evaluate a bioresorbability of AFSs under an environment of an osteoclastic resorption, the AFSs were immersed into the buffer solution consisting of acetic acid and sodium acetate at pH 5.5 as a model of quasi-osteoclastic producting solution. The concentration of calcium ions released from the AFSs in the acetic acid buffer was continuously measured using a calcium ion meter. The profiles of released Ca²⁺ ions indicated that the amounts of Ca²⁺ ions from AFS were more than those of commercially-available HAp powder as a control.