CHANGES OF GLOMERULAR CAPILLARY WALL IN AMINONUCLEOSIDE NEPHROSIS OF RATS

Abstract

Puromycin aminonucleoside (PAN) at a dose of 1.67mg/100 g body weight was injected subcutaneously for 10 days into male Sprague-Dawely rats. The mean urinary protein excretion of the PAN-treated rats was more than 80 times as much as that of the control rats. In light microscopy, swelling and vacuolation of glomerular epithelial cells, and slight thickening of mesangial area were found, but the glomerular basement membrane was not involved. Lysozyme binding was reduced in the lamina rara externa and interna of the glomerular basement membrane and the epithelial surface. Eight sorts of lectins were used to examine alterations in the oligosaccharide chain. Binding of Bauhinia purpurea (BPA) with the capillary wall was markedly increased in PAN-treated rats. The binding of Glycine max (SBA) showed a pattern similar to BPA, but the increment was much less. Thus the alteration of the oligosaccharide chains of the glomerular capillary wall might have a close relationship to the development of proteinuria in PAN-treated rats.