A Handy Field-Portable ELISA System for Rapid Onsite Diagnosis of Infectious Diseases

Harpal Singh; Kazuhiro Morioka; Masayuki Shimojima; Le Van An; Hizuru Nakajima; Akihide Hemmi; Katsumi Uchiyama; Shih Keng Loong; Sazaly AbuBakar; Ming Yang; Masami Sugamata

doi:10.7883/yoken.JJID.2015.417

Short Communication

A Handy Field-Portable ELISA System for Rapid Onsite Diagnosis of Infectious Diseases

Harpal Singh, Kazuhiro Morioka, Masayuki Shimojima, Le Van An, Hizuru Nakajima, Akihide Hemmi, Katsumi Uchiyama, Shih Keng Loong, Sazaly AbuBakar, Ming Yang, Masami Sugamata

Author information

Harpal Singh
Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University
Department of Virology 1, National Institute of Infectious Diseases
Kazuhiro Morioka
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University
Masayuki Shimojima
Department of Virology 1, National Institute of Infectious Diseases
Le Van An
Department of Microbiology, Hue University of Medicine and Pharmacy
Hizuru Nakajima
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University
Akihide Hemmi
Mebius Advanced Technology Ltd.
Katsumi Uchiyama
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University
Shih Keng Loong
Tropical Infectious Diseases Research & Education Center (TIDREC), University of Malaya, Kuala Lumpur, Malaysia (where this work was carried out)
Sazaly AbuBakar
Tropical Infectious Diseases Research & Education Center (TIDREC), University of Malaya, Kuala Lumpur, Malaysia (where this work was carried out)
Ming Yang
Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University
Masami Sugamata
Department of Hygiene and Public Health, Graduate Schools of Human Health Sciences, Tokyo Metropolitan University
Department of Microbiology, Hue University of Medicine and Pharmacy

Keywords: field-portable diagnostics, rapid onsite testing, ELISA, prevention and control, infectious disease

JOURNAL FREE ACCESS

2016 Volume 69 Issue 5 Pages 435-438

DOI https://doi.org/10.7883/yoken.JJID.2015.417

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Abstract

Enzyme-linked immunosorbent assays (ELISAs) are considered the gold standard for the detection of various immunological reactions and can be used for the detection of infectious diseases during outbreaks or in the care of individual patients. To be useful in the timely implementation of prevention and control measures against infectious diseases, a diagnostic modality should be rapid, accurate, and affordable. In the current study, we demonstrate the efficiency (90% less time and volume consumption compared with those of a standard 96-well ELISA), detection capability, and ease of operation of a field-portable, battery-operated ELISA system, approximately the size of a cellular phone (12 × 6 × 5.5 cm), in the serological diagnosis of measles and rubella viruses that has the potential for onsite testing such as during disease outbreaks.

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