Japanese Journal of Infectious Diseases
Online ISSN : 1884-2836
Print ISSN : 1344-6304
ISSN-L : 1344-6304
Short Communication
A Handy Field-Portable ELISA System for Rapid Onsite Diagnosis of Infectious Diseases
Harpal SinghKazuhiro MoriokaMasayuki ShimojimaLe Van AnHizuru NakajimaAkihide HemmiKatsumi UchiyamaShih Keng LoongSazaly AbuBakarMing YangMasami Sugamata
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2016 Volume 69 Issue 5 Pages 435-438


Enzyme-linked immunosorbent assays (ELISAs) are considered the gold standard for the detection of various immunological reactions and can be used for the detection of infectious diseases during outbreaks or in the care of individual patients. To be useful in the timely implementation of prevention and control measures against infectious diseases, a diagnostic modality should be rapid, accurate, and affordable. In the current study, we demonstrate the efficiency (90% less time and volume consumption compared with those of a standard 96-well ELISA), detection capability, and ease of operation of a field-portable, battery-operated ELISA system, approximately the size of a cellular phone (12 × 6 × 5.5 cm), in the serological diagnosis of measles and rubella viruses that has the potential for onsite testing such as during disease outbreaks.

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