Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Mustafa SHUHAIMI; Abdul M. ALI; Norihan M. SALEH; Khatijah YUSOFF; Abdul M. YAZID

doi:10.12938/bifidus1996.20.89

Bioscience and Microflora

Online ISSN : 1349-8355
Print ISSN : 1342-1441
ISSN-L : 1342-1441

Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Mustafa SHUHAIMI, Abdul M. ALI, Norihan M. SALEH, Khatijah YUSOFF, Abdul M. YAZID

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Keywords: bifidobacteria, differentiation, RAPD

JOURNAL FREE ACCESS

2001 Volume 20 Issue 3 Pages 89-94

DOI https://doi.org/10.12938/bifidus1996.20.89

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Abstract

A total of 17 Bifidobacterium pseudocatenulatum strains isolated from infant faeces and 1 Bifidobacterium pseudocatenulatum type strain were compared for their clonal relatedness. Clonal relatedness was compared by randomly amplified polymorphic DNA analysis (RAPD) using three selected primers (GEN 1-60-03, GEN 1-60-06 and GEN 1-60-07). RAPD patterns with DNA fragments ranging from approximately 0.3 to 10.0 kb in size were observed. Examination of the DNA fingerprints using cluster analysis showed a significant genetic diversity among the strains tested.

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