Abstract
A sandwich-type enzyme immunoassay for human chorionic gonadotropin (hCG) was developed with anti hCG-β antibody-enzyme complex and the immobilized solidphase antibody which was prepared from the antiserum to the synthetic peptide corresponding to the carboxyl-terminal region of the β-subnit of hCG (hCG-β).
This assay system showed a high sensitivity equal to the radioimmunoassay and did not cross-react with human luteinizing hormone (hLH), human folicle stimulating hormone (hFSH) or β-subunit of hCG (hCG-β).
The coefficients of variation in within-run and between-run were less than 10% and there was a good correlation between the enzyme immunoassay and the radioimmunoassay of serum sample with a regression eqnation of y=1.038×-0.647 and a correlation coefficient of r=0.953.
