Abstract
In order to clarify the relationships between actomyosin formation and temperature, the viscosity changes of actomyosin were studied under various temperatures. At the same time, the solubility of superprecipitated actomyosin in various KCl solutions was measured under different temperatures. The results were as follows:
1) Actin, myosin and actomyosin all showed negative temperature coefficients for their viscosity.
2) Actomyosin showed no spontaneous dissociation at low temperature.
3) The viscosity response of actomyosin caused by the addition of ATP was most intense at 0°C, becoming gradually less intense as temperature rose.
4) While the splitting time and the course of the recovery phase were prolonged at low temperature, they were shortened at high temperature.
5) Superprecipitated actomyosin was dissolved above 0.4 M KCl solution both at 0°C. and at 25°C.
6) From the above mentioned observations, it was considered that the reaction of actomyosin formation is exothermic.
