Antioxidative Behavior of Phospholipids for Polyunsaturated Fatty Acids of Fish Oil. II

Synergistic Effect of Phospholipids for Tocopherol

Abstract

The antioxidative and synergistic effects of glycerophospholipids (PL) such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidil-inositol (PI) were investigated using fish oil as a substrate at 30°C in the dark.
PC was noted to express antioxidative effect, PE strong synergistic effect, PG proxidative effect and PI to have no effect at all. During autoxidation, mixed tocopherols added to the substrate were consumed in the order, α-, γ-δ-isomers, with or without PL. After the α-isomer disappeared, the γ-isomer started to be consumed, and then the δ-isomer. The remaining time of each tocopherol isomer in the PC or PE-containing substrate was prolonged 1.5 or 3 times that of the control group, respectively.
The consumption rate of each tocopherol isomer was considered less in the substrate with PL than that of the control group because nitrogen-containing PL molecules, especially PE, easily release a hydrogen radical from the amino group to regenerate the corresponding tocopherol from tocopheryl radicals.