Mapping of the Grh1 locus for Green Rice Leahopper Resistance in Rice Using RFLP Markers

Abstract

We determined the chromosomal location of a green rice leafhopper(GRLH) resistance gene, Grh1, using restriction fragment length polymorphism (RFLP) markers. First, we determined the graphical genotype of Norin-PL2, to which Grh1 was introgressed from an indica variety, Pe-bi-hun, and identified four Pe-bi-hun-derived chronrosonral segments on chromosomes 5, 11 and 12. Then, we examined the linkages between Grh1 and RFLP markers located in the four introgressed segments using 108 B₁F₁ plants. The results indicated that the resistance gene Grh1 was located on chromosome 5 and linked to RFLP markers R566, C309, R2558, XNpb260.