Proceedings of the Japan Academy, Series B
Online ISSN : 1349-2896
Print ISSN : 0386-2208
ISSN-L : 0386-2208
Original Papers
An electron microscopic study of the archaeal feast/famine regulatory protein
6. The cylindrical assembly form
Sanae A. ISHIJIMALester CLOWNEYMasashi SUZUKI
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2004 Volume 80 Issue 5 Pages 236-243

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Abstract
Using a microcrystal of the feast/famine regulatory protein (FFRP) pot0434017 (FL11), a cryo-electron micrograph was taken, showing a projection of cylinder-like assemblies packed parallel to each other. This electron micrograph was processed in the Fourier space by selecting spots reflecting the packing and, in addition, those reflecting stacking of units inside the cylinders. Twenty seven subimages were selected, each containing three cylinders of 24 discs each, running nearly parallel to each other. By averaging these images and in combination with another average showing a different view [Ishijima, S. A., Clowney, L., and Suzuki, M. (2004) Proc. Jpn. Acad., Ser. B 80, 183-188], some details of the 3D structure of the cylindrical assembly form are discussed.


(Communicated by Masanori OTSUKA, M.J.A.)
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© 2004 The Japan Academy
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