Abstracts of the Annual Meeting, The Japanese Society of Sericultural Science

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Influence of high temperature treatment of the silkworms on dimorphic spermatogenesis

Naoko Yamashiki, Ken Sahara

Session ID: 329
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.101.0

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Actin molecules are responsible for peristaltic squeezing in Bombyx spermatogenesis

Ken Sahara, Naoko Kawamura

Session ID: 330
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.102.0

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Chorion architecture in the japanese giant silkmoth, Caligura japonica japonica Moor

Yutaka kawaguchi, Masatoshi Ichida, Takahiro Kusakabe, Katsumi Koga

Session ID: 331
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.103.0

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Minos-mediated germ-line tranformation of the silkworm, Bombyx mori

Morikazu Imamura, Kayo Shimizu, Guo Xing Quan, Katsura Kojima, Toshio ...

Session ID: 332
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.104.0

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Genomic structure and expression analysis of the gene encoding a basic Kunitz-type Chymotrypsin inhibitor of Bombyx Mori

Ningjia He, Hiroshi Fujii, Yutaka Banno, Kohji Yamamoto

Session ID: 333
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.105.0

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Kunitz-type chymotrypsin inhibitor CIb1 of silkworm Bombyx mori is a basic peptide consisting of 62 amino acid residues. To investigate the physiological roles of this peptide, we cloned it for genomic structure analysis. CIb1 cDNA was used as a probe to screen a BAC sub-library. One positive clone containing the upstream sequences was isolated and the sequence result showed that CIb1 gene consists 3 exons spaced by 2 introns. Consensus TATA and CCAAT boxes were identified in the 5'-flanking region. Other binding sites for transcription factors such as NF-kB, GATA, C/EBP, COUP-TF/HNF-4, SRY, and HOXA3 were also detected. Southern blotting suggested a single copy of CIb1 gene in the silkworm genome. Northern blotting indicated that the expression of CIb1 gene is transcriptionally regulated during development and apparently tissue specific. Together our data, the transcription factors putatively responsible for the roles of CIb1 in silkworm hemolymph were discussed.

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Analysis of gene expression profiles in wing discs of Bombyx mori during metamorphosis

Manabu Ote, Kazuei Mita, Hideki Kawasaki, Motoaki Seki, Masahiko Kobay ...

Session ID: 334
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.106.0

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comparison of gene expression in diapause and non-diapause eggs using cDNA microarrays

yuko suesada, manabu ote, motoaki seki, kazuei mita, toru shimada

Session ID: 335
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.107.0

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Diapause hormone of Bombyx mori induces unusual wing melanization of the butterfly, Eurema hecabe

Yoshiomi Kato, Yumiko Ishii, Kunihiro Shiomi

Session ID: 336
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.108.0

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Seasonal morph determination of the butterfly Eurema hecabe is controlled by photoperiod experienced during larval stage, but its hormonal regulation has been unknown till recent years. Recently, Ishii et al. (2002) demonstrated that summer moph producing hormone, which is a neuropeptide, is involved in the determination of seasonal wing morph. Therefore, we tested whether diapause hormone (DH) of Bombyx mori is effective for the summer morph production of E. hecabe As a result, diapause hormone failed to induce summer morph when applied to autumn morph producing pupae, but unusual and curious melanization was induced on the forewing upperside of the adults obtained. Furthermore, DH may have a possibility to stimulate ovarian maturation in diapause-destined autumn morph females although we must do further experiments. This is the first report where DH effect is found in other insect species except B. mori.

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Taste-induced swallowing in the larvae of silkworms

KEN SASAKI, KIYOSHI ASAOKA

Session ID: 337
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.109.0

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Consideration of the evolutional history of wild silk moth Bombyx mandarinaby comparing the pattern of mariner-like-element among some specimens now inhabiting in different Asian area

YUMIKO NAKAJIMA, HIROFUMI FUJIMOTO, TAKASHI NAKAMURA, YUTAKA BANNO, KA ...

Session ID: 338
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.110.0

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PCR against genomic DNA from individuals of Bombyx mandarina distributed in three areas of east Asia, i.e., Japan, Korea and China, was performed with the inverted terminal repeats of the Hyalophora cecropia MLE as a primer. Each specimen gave several bands with different sizes. One individual from China had a discrete 4.2-kb band containing a BMC1 retrotransposon, which is a unique fragment detected in almost all of the silkworm races and strains. Thus, the 4.2-kb unit might provide a clue for addressing the issue about the segregation of B. mori genome. From the phylogenetic analysis, MLE (BmTNML ) of 4.2kb band and 2.5kb band from the specimen of Fukuoka had high similarity each other supporting the idea that this type of MLE is an ancient type before segregation of B. mandarina among different chromosome numbers, i.e.,27 and 28.

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Adhesion and virulence of Metarhizium anisopliae conidia to insect.

Ryozaburo Iwase, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu

Session ID: 401
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.111.0

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Pathogenicity of gacA mutant of Pseudomonas aeruginosa to Bombyx mori.

Yuuka Chieda, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Jae Man Lee, Takah ...

Session ID: 402
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.112.0

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Comparison of the susceptibility to Beauveria brongniartii among silkworm, Bombyx mori strains by injection

Sanae Wada, Tatsuo Mikuni, Hirotada Tamura, Wataru Mitsuhashi, Kazuhis ...

Session ID: 403
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.113.0

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Isolation of the nonpathogenic pebrine disease pathogen,Nosema bombycis to the silkworm.

HIDETOSHI IWANO, ASUKA TOMIYAMA, TOSIHIKO HUKUHARA, YOSHINORI HATAKEYA ...

Session ID: 404
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.114.0

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Functional analysis of ORF2 proteins of novel Cry proteins, Cry39A and Cry40A

Takeshi Ito, Ken Sahara, Shinichiro Asano, Hisanori Bando

Session ID: 405
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.115.0

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Localization of BmNPV virion in Sf9 cells

Yasuhiro Katou, Motoko Ikeda, Michihiro Kobayashi

Session ID: 406
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.116.0

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Identification of a HycuNPV gene that inhibits BmNPV replication in BmN-4 cells

Noriko SHIRATA, Motoko IKEDA, Michihiro KOBAYASHI

Session ID: 407
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.117.0

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The role of cellular PCNA in pcna-defective AcMNPV mutants-infected Sf9 cells

Satoko IWAHORI, Michihiro KOBAYASHI, Motoko IKEDA

Session ID: 408
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.118.0

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Characterization of scarabaeidcidal cry genes

SHINICHIRO ASANO, MIKIKO NOZAWA, KEN SAHARA, HISANORI BANDO

Session ID: 410
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.119.0

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The interactions between Cry1A toxins and APN isoforms of Bombyx mori

masatsugu kitamura, ken sahara, shinitirou asano, hisanori bando

Session ID: 410
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.120.0

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Characterization and biological activity of enterotoxins (NHE) from B.thuringiensis HD12

MAYUKO KAWAMURA, KEN SAHARA, SHINICHIRO ASANO, HISANORI BANDO

Session ID: 411
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.121.0

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Analysis of Receptor Binding Site of Bacillus thuringiensis Insecticidal Protein Cry1Aa by Site-directed Binding Inhibition.

Yukino Inoue, Shogo Atsumi, Masashi Iizuka, Ryoichi Sato

Session ID: 412
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.122.0

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Benefits of analysis of characteristics of Japanese improved silkworm races for reeling technology

HARUO KINOSHITA, EIICHI KOSEGAWA, MASAHIKO HIROKAWA, KEN-ICHIROU TATEM ...

Session ID: 413
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.123.0

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Bulkiness control mechanism of High Bulk Silk in the silk reeling

Chiyuki Takabayashi, Kuniko Nakamura, Eiko Miyazaki

Session ID: 414
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.124.0

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Development of the raw silk size management system

Kenichi NAKAJIMA

Session ID: 415
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.125.0

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Development of Robot in imitation of Spinning behavior by Silkworm

Hideaki Morikawa, Mikihiko Miura, Takashi Kawamura, Masayuki Iwasa

Session ID: 416
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.126.0

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Ultra-fine Silk Fabrics

Yoshiko Mgoshi, Mary Becker, Kiyoshi Watanabe

Session ID: 417
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.127.0

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Ultra-fine transparent silk Fabrics were woven using 10 denier silk filaments produced by a strain of silkworms breed at the Okaya branch of NIAS. Using these ultra-fine filaments, fabrics having a weight/area ratio of 12-13g/__m2__ were able to be produced. Several other silk fabrics were woven with synthetic filaments that were removed by heat treatment, leaving only the silk. The characteristics of these fabrics will be discussed.

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Microstructure and the additional value of undeveloped silk

HIROMU AKAI, TAKAYUKI NAGASHIMA

Session ID: 418
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.128.0

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Diameter and refractive index of a fibroin filament determined by scattered light pattern

Kouji Sakata, Fumiaki Tajima, Yoshio Ishiguro, Masanori Miyashita, Yos ...

Session ID: 419
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.129.0

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Conformational analysis of spider fibroin using microATR/FT-IR

Mitsuhiro Miyazawa

Session ID: 420
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.130.0

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Secondary structure and folding analyses of cocoonase

Mitsuhiro Miyazawa, Keiji Kurata

Session ID: 421
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.131.0

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Secondary Structure of Liquid Sericin and its Structural Changes with Dehydration

Hidetoshi Teramoto, Mitsuhiro Miyazawa

Session ID: 422
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.132.0

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The functional characteristic of bombyx mori and wild cocoon's sericin

Katsutoshi YASUDA, Masayoshi YAMAZAKI, Toshio YAMAMOTO

Session ID: 423
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.133.0

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Disolution of sericin in cocoon shell of the silkworm race "Sericin-hope" by heat water

TAKAKO MIYAJIMA, TOSHIO YAMAMOTO, KEISUKE MASE, TETSUYA IIZUKA, YOUKO ...

Session ID: 424
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.134.0

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Enhancement of the proliferation of human fibroblasts by sericin

Kozo Tsubouchi, Yoko Takasu, Yumiko Igarashi, Hiromi Yamada

Session ID: 425
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.135.0

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Amino acid sequences of sericin-derived peptides

Yoko Takasu, Hitoshi Saito, Hiromi Yamada, Yumiko Igarashi, Kozo Tsubo ...

Session ID: 426
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.136.0

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Extraction of lipid from silkworm larva by supercritical carbon dioxide

Atsunobu Haga, Zbigniew Draczynski, Mitsuhiro Miyazawa

Session ID: 427
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.137.0

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The effects of malberry leaves, or various amounts of fertilizer for the cocoon quality of the silkworm race "Hakugin"

takahisa ichihashi, toshio yamamoto

Session ID: 428
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.138.0

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structure and tissue localization of polysaccharide from mulberru laeves

Hisato Katayama, Ayako Kida, ikuro Nitta, Siji Miyata, Toshiharu Furus ...

Session ID: 429
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.139.0

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Analysis of downstream region of rbcL gene in Moraceae

sakihito kitajima, yuuji matsuda, yukio sugimura

Session ID: 430
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.140.0

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Analysis of response to drought stress in mulberry.

Sakihito Kitajima, Ativetin Panida, Toshiharu Furusawa, Yukio Sugimura

Session ID: 431
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.141.0

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Computational search for viral RNA structures using PatScan search program.

Yoshinori Hatakeyama, Norihiro Shibuya, Takashi Nishiyama, Nobuhiko Na ...

Session ID: 432
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.142.0

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Isolation and characterization of bacteria-like chitinase genes from several species of lepidopteran insects

Takaaki Daimon, Kazuei Mita, Toru Shimada

Session ID: 433
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.143.0

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Identifying the tissues expressing cecropin B in a transgenic silkworm

Kiyoko Taniai, Toshio Kanda, Toshiki Tamura, Morikazu Imamura

Session ID: 434
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.144.0

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Except for hemocytes and fat bodies, insect immune tissues have not been carefully determined. To identify the organs that express cecropin B in silkworms, we constructed a transgenic silkworm that was transformed with the green fluorescent protein (GFP) gene under the control of an 800-bp CecB2 promoter region and followed by the 540-bp 3'-UTR of CecB2 using a transposon, piggyBac. In the transgenic silkworms, little GFP was detected in normal larvae, while a strong GFP signal was observed in the fatbodies, hemocytes, and tracheae in the head and testis after bacteria injection. Our finding that infection induces an antibacterial peptide in generative cells in insects is new.

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A silkworm paralytic peptide mRNA has a potential for a polycistronic mRNA.

yasushi kanamori, manabu kamimura

Session ID: 435
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.145.0

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host range factor 1 (Ld-hrf1) gene from LdMNPV promotes NPVs replication in non-permissive Ld652Y cells

hiroki ishikawa, motoko ikeda, C. A. Felipe Alves, michihiro kobayash ...

Session ID: 436
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.146.0

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Ubiquitin ligase activities of Bombyx mori nucleopolyhedrovirus RING finger proteins

Noriko Imai, Shogo Matsumoto, WonKyung Kang

Session ID: 437
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.147.0

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Identification of inhibitor of apoptosis protein (IAP) from Lepidoptera

yasuhiro sugiyama, motoko ikeda, satoko iwahori, hiroki ishikawa, mich ...

Session ID: 438
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.148.0

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Identification of a nobel effector caspase in Lepidoptera

kazunori okamoto, hiroki ishikawa, motoko ikeda, michihiro kobayashi

Session ID: 439
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.149.0

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