Abstracts of the Annual Meeting, The Japanese Society of Sericultural Science

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Introduction of a cecropin B gene from Bombyx mori into rice genome and analysis of gene expression

Minoru Yamakawa, Ryoko Sawahata, Kazuko Ono, Hiroshi Tanaka, Tatsuhito ...

Session ID: 214
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.51.0

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analysis of promoter activities of B. mori antibacterial genes by LPS treatment

Seiichi Furukawa, Hiromitsu Tanaka, Hiroshi Nakazawa, Minoru Yamakawa

Session ID: 215
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.52.0

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Isolation of antiviral protein

Hiroshi Nakazawa, K. M. Ponnuvel, Seiichi Furukawa, Hiromitsu Tanaka, ...

Session ID: 216
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.53.0

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Interaction between host transcription factors and IE1, an essential regulatory protein of baculovirus

TAKESHI OONO, SHIN-ICHIRO ASANO, KEN SAHARA, HISANORI BANDO

Session ID: 217
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.54.0

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Cloning and expression of Bombyx Broad-Complex gene

Hisayo Urakawa, Makio Takeda, Yoshihiro Fujiwara

Session ID: 219
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.55.0

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Molecular cloning and expression analysis of silkworm Broad-Complex

A.M.S Reza, Yasushi Kanamori, Kyoko Kikuchi, Sachiko Shimura, Tetsuro ...

Session ID: 219
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.56.0

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Molecular cloning of cDNA of Rab from the brain of Bombyx mori and its expression

TOMOHIDE UNO, SUSUMU HIRAGAKI

Session ID: 220
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.57.0

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cDNAs were isolated from the brain of Bombyx mori using RT-PCR.Four clones are known , and 4 clones are unknown.Full-length cDNA was obtained by RACE and silk base. Rab was expressed in E.coli and purified. The expressed protein showed GDP binding activity.

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Functional analysis of BmUbx by RNAi

Teruyuki NIIMI, Okitsugu YAMASHITA, Toshinobu YAGINUMA

Session ID: 221
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.58.0

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Cloning and analysis of a-,and b-tubulin genes of Bombyx mori using an EST database of Bombyx mori

Hideki kawasaki, Kimihiko Sugaya, Guo-Xing Quan, junko Nohata, Kazuei ...

Session ID: 222
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.59.0

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Analysis and of Bombyx mori hsp70 gene promoter

Katsura KOJIMA, Guo-Xing QUAN, Morikazu IMAMURA, Toshio KANDA, Junko N ...

Session ID: 223
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.60.0

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Molecular cloning of Clock and NAT of Antheraea pernyi

TAKETO TSUGEHARA, YOSHIHIRO FUJIWARA, MAKIO TAKEDA

Session ID: 224
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.61.0

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Analysis of silkworm gene products by mass spectrometry

Hideyuki Kajiwara, Kazuei Mita, Masatoshi Nakamura, Masumi Ishizaka

Session ID: 225
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.62.0

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In the studies of developmental gene expressions in a silkworm, it is necessary to attempt exhaustive protein analysis, proteomic analysis, with EST analysis. We started the silkworm proteomics using Daizo which has been studied EST. Middle silkgrand, posterior silkgrand, midgut, fat body and other tissues were collected from fifth-instar day-3 and 4 larva. Proteins were separated by a two-dimensional polyacrylamide gel electrophoresis and the each spot was digested by a trypsin after the alkylation. Peptides were analyzed by an on-line capillary HPLC-MS (LCQ Deca). The data obtained by MS analysis were applied to the program Mascot and Sequest for the identification of the proteins using the genome data from fruit fly and the translated data from silkworm EST. Many genes encoding protein were identified by MS analysis. Some of them were not hit to fruit fly genome data but did to silkworm EST. Additionally, artificial shift of reading frame in EST nucleotide sequences were found by MS analysis.

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Isolation and comparison of ecdysone responsive cuticle protein genes in Bombyx wing disc

Takanori Noji, Manabu Oote, Masahisa Takeda, Kazuei Mita, Toru Shimada ...

Session ID: 226
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.63.0

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Detection of methylated DNA in Bombyx mori

Ken-ichiro TATEMATSU, Masahiko HIROKAWA, Eiichi KOSEGAWA

Session ID: 227
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.64.0

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L-chain Fibroin Genes of Trichopteran Insects

Naoyuki YONEMURA, Kazuei MITA, Toshiki TAMURA, Frantisek SEHNAL

Session ID: 228
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.65.0

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Detection of single nucleotide polymorphism in domesticated silkworm by direct sequencing method

Eiichi KOSEGAWA, Ken-ichiro TATEMATSU, Masahiko HIROKAWA

Session ID: 229
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.66.0

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The establishment of the basic technology for the cultivar identification is desired for race patent acquisition of the domesticated silkworm, Bombyx mori. Then, we aim at the development race-identification method using single nucleotide polymorphism (SNPs).
No information of the SNPs in silkworm genome is not known. So we tried to detect SNPs by direct sequencing method. Then, the primer was designed from BmHSC70-4 gene (DDBJ: AB084923), and the nucleotide sequence was analyzed. Consequently, 1.7kb of fragment was amplified by PCR using genomic DNA as a template. As the result of the comparison among 14 races, 32 SNPs were detected. These SNPs consisted of 14 transitions and 7 transversions. In the meantime, the frequency of SNP site per unit genome DNA length seemed to be high. SNP sites were detected only in intronic region. SNPs were slightly classified into two types of haplotype except for several race specific SNP sites. From this fact, it seemed to be possible to type this gene by representing one or several SNPs.

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Screening and isolation of the genes involved in DNA double-strand break repair using an RNAi strategy on silkworm

HARUNA TSUKIOKA, TAKAHIRO KUSAKABE, KAZUHIRO OKANO, KAZUEI MITA, TORU ...

Session ID: 230
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.67.0

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Isolation and expression analysis of BmVLG

hajime Nakao, tosio kanda, yuzi yasukochi, toshiki tamura

Session ID: 231
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.68.0

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Construction of the protein expression system using neuromedin U receptor homolog cDNAs isolated from Bombyx ovaries

Takashi Honma, Mari Kanba, Teruyuki Niimi, Okitugu Yamashita, Toshinob ...

Session ID: 232
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.69.0

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Tissue Distribution of Y-box Protein mRNA and Temporal Pattern in the Ovary Development of Silkworm, Bombyx mori

Taro Okada, Ningjia He, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii

Session ID: 233
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.70.0

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Drastic enhancement of AcNPV propagation in Antheraea pernyi cell line by altering culture medium.

Kenichi Maegawa, Jun Kobayashi, Tetsuro Yoshimura

Session ID: 234
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.71.0

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Molecular evolution of mitochondrial genomes of Bombyx mori, B. mandarina, and Saturniidae moths

Kenji Yukuhiro, Hideki Sezutsu

Session ID: 235
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.72.0

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comparison of the mitochondrial gene in the silkworm and wild mulberry silkworm, Bombyx mori and mandarina.

Hideaki Yamamoto, Osamu Ninagi, Manabu Yokoyama, Tosikazu Osiki, Wajir ...

Session ID: 236
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.73.0

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Distribution chart of the mixed population containing two strains different on dispersal behavior at new hatched larvae of silkworm, bombyx mori.

Haruyoshi Otsuki, Osamu Ninagi, Takeshi Yokoyama, Toshikazu Oshiki, Wa ...

Session ID: 237
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.74.0

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Analysis of embryogenesis of the silkworm, Bombyx mori, using heavy ion beams: Induction of split germ bands

Masamichi Kuwakubo, Kana Fukamoto, Koji Shirai, Rensuke Kanekatsu, Ken ...

Session ID: 301
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.75.0

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Developmental change of casein kinase 2 activity in the silkworm eggs.

TAKAYUKI YAMAMOTO, HIROSHI SAWADA, TOMOMI KATOH, MOTOKO NAKAGOSHI, KEI ...

Session ID: 302
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.76.0

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Molecular cloning and characterization of a cDNA encoding a novel RNA helicase like protein in the silkworm eggs.

Hiroshi Sawada, Takayuki Yamamoto, Tomomi Kato, Motoko Nakagoshi, Susu ...

Session ID: 303
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.77.0

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The carbohydrate metabolism conversion and regulation in embryonic development of Bombyx mori.

Daisuke Sakano, Aya Konishi, Eiji Kotani, Yukio Sugimura, Toshiharu Fu ...

Session ID: 304
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.78.0

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Histological distribution of BmEts mRNA, a diapause-specific mRNA, in diapause and non-diapause embryos of the silkworm, Bombyx mori

Tsuyoshi Furukawa, Hisaya Sotoshiro, Toru Shimada, Masahiko Kobayashi

Session ID: 305
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.79.0

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Trial to search genes related to initiation/maintenance of embryonic diapause: Bombyx gene homologous to hOXR1 and some candidate genes

Masaki Takahashi, Teruyuki Niimi, Toshinobu Yaginuma

Session ID: 306
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.80.0

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PCR detection of genomic DNA strand breaks by radiation irradiation

Mai MIZOROGI, Emiko YAMAUCHI, Hirofumi FUJIMOTO, Kazuo HASHIDO, Kozo T ...

Session ID: 307
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.81.0

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Detection of DNA strand breaks using real time PCR for DNA structure analysis

Emiko YAMAUCHI, Mai MIZOROGI, Hirofumi FUJIMOTO, Ritsuko WATANABE, Kim ...

Session ID: 308
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.82.0

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Does female Bmdsx protein control the development of genital disc?

Shunsuke Funaguma, Masataka Suzuki, Toshio Kanda, Toshiki Tamura, Toru ...

Session ID: 309
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.83.0

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Expression of male Bmdsx protein in females causes defects in female differentiation.

Masataka Suzuki, Shunsuke Funakuma, Toshio Kanda, Toshiki Tamura, Shog ...

Session ID: 310
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.84.0

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Studies on sex-specifically expressed genes in the early embryo of the silkworm, Bombyx mori

Michiyoshi TAKAHASHI, Motoaki SEKI, Hisaya SOTOSHIRO, Toru SHIMADA

Session ID: 311
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.85.0

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Genome-wide search for sex-specific genes in early embryo of Bombyx mori.

Motoaki SEKI, Michiyoshi TAKAHASHI, Manabu OTE, Masataka G. SUZUKI, Ka ...

Session ID: 312
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.86.0

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Features and property of Kobuchizawa saving silkworm races on the inducible antibacterial activity in the hemolymph.

Eiji okada, Eiichi Kosegawa, Masahiko Hirokawa, Ken'ichirou Tatematsu ...

Session ID: 313
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.87.0

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Production of the antibacterial raw silk race by introduction of GAL4/UAS-Systems

Nobuo KUWABARA, Morikazu IMAMURA, Toshio KANDA, QUAN GUO-XIMG, Minoru ...

Session ID: 314
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.88.0

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Breeding and maintenance of mutant silkworm strains with a common genetic background.

Rensuke Kanekatsu, Koji Shirai, Kenji Kiguchi

Session ID: 315
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.89.0

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Translocation of the Z chromosome to the W chromosome marked with the Moricaud gene in the silkworm

AKIO OHNUMA

Session ID: 316
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.90.0

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Dosimetry for high dose gamma-ray irradiated silkworm

Emiko YAMAUCHI, Mai MIZOROGI, Hirofumi FUJIMOTO, Kazuo HASHIDO, Kozo T ...

Session ID: 317
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.91.0

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Genetic analysis of a new mutant gene "maternal brown egg of Shimizu" with gene symbol b-2^s

Kunimitu Shimizu, Masahiko Hirokawa, Ken-ichiro Tatematsu, Eiichi Kose ...

Session ID: 318
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.92.0

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A new MITE Organdy insertion causes the ogt translucent larval skin mutation.

Natuo KOMOTO, Hideki SEZUTSU, Kenji YUKUHIRO, Yutaka BANNO, Hiroshi FU ...

Session ID: 319
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.93.0

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The order of Nc, E^Al, E^Nc and E^Mu genes located on the sixth linkage group of the silkworm, Bombyx mori L. (Preliminary report)

Masahiko Hirokawa, Ken-ichiro Tatematsu, Kunimitsu Shimizu, Eiichi Kos ...

Session ID: 320
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.94.0

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Construction and evaluation of a whole genome shotgun library in Bombyx mori

Toru Shimada, Masanari Kitagawa, Kazuei Mita

Session ID: 322
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.95.0

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Simultaneous construction of genetic and physical maps of the silkworm

Yuji Yasukochi, Kotaro Baba, Junko Nohata, Kazuei Mita

Session ID: 323
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.96.0

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Chromosome identification in Bombyx mori using BAC-FISH

atsuo yoshido, sin-ichiro asano, hisanori bando, yuji yasukochi, ken s ...

Session ID: 325
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.97.0

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Mapping of Sawa-J strain Food Preference Gene by Using cDNA-RFLPs.

Tetsuya Iizuka, Keisuke Mase, Toshio Yamamoto, Wajirou Hara

Session ID: 326
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.98.0

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Genome analysis of the BAC contigs covering a densovirus nonsusceptibility gene, nsd-2 of Bombyx mori

Keiko Kadono-Okuda, Shun-ichi Sasanuma, Motoe Sasanuma, Junko Nohata, ...

Session ID: 327
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.99.0

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Analysis of spontaneous parthenogenetic development in silkworm

KAZUMI KIDO, TAKAHIRO KUSAKABE, KAORI KITA, YUTAKA KAWAGUCHI, KATSUMI ...

Session ID: 328
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.100.0

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