Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe

Gongwu SONG; Ling LI; Liming LIU; Guangrong FANG; Shaofang LU; Zhike HE; Yun’e ZENG

doi:10.2116/analsci.18.757

Original Papers

Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)₂PIP(V) as a Nucleic Acid Probe

Gongwu SONG, Ling LI, Liming LIU, Guangrong FANG, Shaofang LU, Zhike HE, Yun’e ZENG

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JOURNAL FREE ACCESS

2002 Volume 18 Issue 7 Pages 757-759

DOI https://doi.org/10.2116/analsci.18.757

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Abstract

A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)₂PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)₂PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.

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