Effective Separation and Simultaneous Determination of Corynoxeine and Its Metabolites in Rats by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Application to Pharmacokinetics and In Vivo Distribution in Main Organs

Wei WANG; Shaozhong LUO; Yaping CHEN; Bo LI; Masao HATTORI

doi:10.2116/analsci.32.705

Notes

Effective Separation and Simultaneous Determination of Corynoxeine and Its Metabolites in Rats by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Application to Pharmacokinetics and In Vivo Distribution in Main Organs

Wei WANG, Shaozhong LUO, Yaping CHEN, Bo LI, Masao HATTORI

Author information

Keywords: Corynoxeine, separation, quantification, pharmacokinetics, brain uptake index

JOURNAL FREE ACCESS

2016 Volume 32 Issue 6 Pages 705-707

DOI https://doi.org/10.2116/analsci.32.705

Details

Abstract

An effective separation and simultaneous determination of corynoxeine and its metabolites using high-performance liquid chromatography with tandem mass spectrometry was developed and validated. The method was applied to pharmacokinetics and in vivo distribution investigations in rats after oral (0.105 mmol kg⁻¹) and intravenous (0.0105 mmol kg⁻¹) doses of corynoxeine. Its brain uptake index was of 3.08 × 10⁻¹¹ mol g⁻¹ at 3 h and 3.75 × 10⁻¹¹ mol g⁻¹ at 74 min after oral and intravenous doses, respectively.

Fullsize Image

Corresponding author