Neuroprotectins A and B, Bicyclohexapeptides Protecting Chick Telencephalic Neuronal Cells from Excitotoxicity I. Fermentation, Isolation, Physico-chemical Properties and Biological Activity

HIROYUKI KOBAYASHI; KAZUO SHIN-YA; KOJI NAGAI; KEN-ICHI SUZUKI; YOICHI HAYAKAWA; HARUO SETO; BONG-SIK YUN; IN-JA RYOO; JUNG-SIK KIM; CHANG-JIN KIM; ICK-DONG YOO

doi:10.7164/antibiotics.54.1013

Neuroprotectins A and B, Bicyclohexapeptides Protecting Chick Telencephalic Neuronal Cells from Excitotoxicity

I. Fermentation, Isolation, Physico-chemical Properties and Biological Activity

HIROYUKI KOBAYASHI, KAZUO SHIN-YA, KOJI NAGAI, KEN-ICHI SUZUKI, YOICHI HAYAKAWA, HARUO SETO, BONG-SIK YUN, IN-JA RYOO, JUNG-SIK KIM, CHANG-JIN KIM, ICK-DONG YOO

Author information

HIROYUKI KOBAYASHI
Institute of Molecular and Cellular Biosciences, The University of Tokyo
KAZUO SHIN-YA
Institute of Molecular and Cellular Biosciences, The University of Tokyo
KOJI NAGAI
Microbiology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd.
KEN-ICHI SUZUKI
Microbiology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd.
YOICHI HAYAKAWA
Institute of Molecular and Cellular Biosciences, The University of Tokyo
HARUO SETO
Institute of Molecular and Cellular Biosciences, The University of Tokyo
Present address: Faculty of Applied Bio-Science, Tokyo University of Agriculture
BONG-SIK YUN
Korea Research Institute of Bioscience and Biotechnology
IN-JA RYOO
Korea Research Institute of Bioscience and Biotechnology
JUNG-SIK KIM
Korea Research Institute of Bioscience and Biotechnology
CHANG-JIN KIM
Korea Research Institute of Bioscience and Biotechnology
ICK-DONG YOO
Korea Research Institute of Bioscience and Biotechnology

JOURNAL FREE ACCESS

2001 Volume 54 Issue 12 Pages 1013-1018

DOI https://doi.org/10.7164/antibiotics.54.1013

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Abstract

Glutamate, an excitatory amino acid, is known to induce neurotoxicity in central nervous system under abnormal conditions such as ischemia, hypoglycemia, epilepsy, Huntington's chorea, Parkinson's disease and Alzheimer's disease. In our search for neuroprotective agents of microbial origin against excitatory neurotoxins, we have isolated two new bicyclohexapeptides, neuroprotectins A and B, together with a known compound complestatin, from the fermentation broth of Streptomyces sp. Q27107. Neuroprotectins protected primary cultured chick telencephalic neurons from glutamate- and kainate-induced excitotoxicities in a dose-dependant fashion.

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