Serotonin Derivative, N-(p-Coumaroyl)serotonin, Isolated from Safflower (Carthamus tinctorius L.) Oil Cake Augments the Proliferation of Normal Human and Mouse Fibroblasts in Synergy with Basic Fibroblast Growth Factor (bFGF) or Epidermal Growth Factor (EGF)

Takemasa Takii; Masaru Hayashi; Hiroyuki Hiroma; Taku Chiba; Sachiko Kawashima; Hui Li Zhang; Akito Nagatsu; Jinsaku Sakakibara; Kikuo Onozaki

Serotonin Derivative, N-(p-Coumaroyl)serotonin, Isolated from Safflower (Carthamus tinctorius L.) Oil Cake Augments the Proliferation of Normal Human and Mouse Fibroblasts in Synergy with Basic Fibroblast Growth Factor (bFGF) or Epidermal Growth Factor (EGF)

Takemasa Takii, Masaru Hayashi, Hiroyuki Hiroma, Taku Chiba, Sachiko Kawashima, Hui Li Zhang, Akito Nagatsu, Jinsaku Sakakibara, Kikuo Onozaki

Author information

Takemasa Takii
Faculty of Pharmaceutical Sciences, Nagoya City University
Masaru Hayashi
Faculty of Pharmaceutical Sciences, Nagoya City University
Hiroyuki Hiroma
Faculty of Pharmaceutical Sciences, Nagoya City University
Taku Chiba
Faculty of Pharmaceutical Sciences, Nagoya City University
Sachiko Kawashima
Faculty of Pharmaceutical Sciences, Nagoya City University
Hui Li Zhang
Faculty of Pharmaceutical Sciences, Nagoya City University
Akito Nagatsu
Faculty of Pharmaceutical Sciences, Nagoya City University
Jinsaku Sakakibara
Faculty of Pharmacy, Meijo University
Kikuo Onozaki
Faculty of Pharmaceutical Sciences, Nagoya City University

Keywords: coumaroyl serotonin, EGF, FGF, fibroblast, growth factor

JOURNAL FREE ACCESS

1999 Volume 125 Issue 5 Pages 910-915

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Abstract

N-(p-Coumaroyl)serotonin (CS) with antioxidative activity is present in safflower oil. We have reported that CS inhibits proinflammatory cytokine generation from human monocytes in vitro. As reactive oxygen species (ROS) affect cell proliferation, in this study the effect of CS on the proliferation of various cell types was examined. CS augments the proliferation of normal human and mouse fibroblast cells. The cells continue to proliferate in the presence of CS and form a transformed cell-like focus without transformation. CS, however, does not augment the proliferation of other cell types, either normal or tumor cells. CS augments the proliferation of fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF), but not with acidic FGF(aFGF) or platelet-derived growth factor (PDGF). This study using synthesized derivatives of CS reveals that the growth-promoting activity is not due to antioxidative activity. These findings indicate that CS is a natural compound with unique growth-promoting activity for fibroblasts.

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