SHORT NOTES
Molecular sexing using DNA extracted from the natal down of the Grey-headed Lapwing Vanellus cinereus
2014 Volume 63 Issue 1 Pages 43-47
Details
2014 Volume 63 Issue 1 Pages 43-47
To establish a simple and strict method for molecular sexing of Grey-headed Lapwing chicks, we attempted to extract DNA from natal down. Sex-linked CHD genes were successfully amplified with the DNA samples as templates in PCR. DNA extracted from natal down was shown to be sufficient for this purpose. Using this method, of 30 chicks from 17 nests, each of 14 females and 16 males were clearly sexed. Obtaining DNA from natal down is not only easy for researchers, but also one of the least invasive methods for the study of chicks.
