Trans-Resveratrol Inhibits Early Blood Vessel Formation (Vasculogenesis) Without Impairment of Embryonic Growth

Paulo Fernando Dias; Fernanda Vieira Berti; Jarbas Mota Siqueira Jr; Marcelo Maraschin; Antônio Ricardo Gagliardi; Rosa Maria Ribeiro-do-Valle

doi:10.1254/jphs.FP0071876

Full Papers

Trans-Resveratrol Inhibits Early Blood Vessel Formation (Vasculogenesis) Without Impairment of Embryonic Growth

Paulo Fernando Dias, Fernanda Vieira Berti, Jarbas Mota Siqueira Jr, Marcelo Maraschin, Antônio Ricardo Gagliardi, Rosa Maria Ribeiro-do-Valle

Author information

Paulo Fernando Dias
Laboratory of Medicinal Plants Pharmacology, Department of Pharmacology, Biological Sciences Center (CCB), Federal University of Santa Catarina (UFSC), Brazil
Laboratory of Bioactivity Studies and Animal Morphogenesis, Department of Cell Biology, Embryology and Genetics, Biological Sciences Center (CCB), Federal University of Santa Catarina (UFSC), Brazil
Fernanda Vieira Berti
Laboratory of Bioactivity Studies and Animal Morphogenesis, Department of Cell Biology, Embryology and Genetics, Biological Sciences Center (CCB), Federal University of Santa Catarina (UFSC), Brazil
Jarbas Mota Siqueira Jr
Laboratory of Medicinal Plants Pharmacology, Department of Pharmacology, Biological Sciences Center (CCB), Federal University of Santa Catarina (UFSC), Brazil
Marcelo Maraschin
Plant Morphogenesis and Biochemistry Laboratory, Department of Plant Science, Agrarian Sciences Center (CCA), Federal University of Santa Catarina (UFSC), Brazil
Antônio Ricardo Gagliardi
Department of Physiology, Faculty of Medical Sciences of Santos, Lusíada University Center (UNILUS), Brazil
Clinical Unit of Dyslipidemias, São Paulo Heart Institute, São Paulo University, Brazil
Rosa Maria Ribeiro-do-Valle
Laboratory of Medicinal Plants Pharmacology, Department of Pharmacology, Biological Sciences Center (CCB), Federal University of Santa Catarina (UFSC), Brazil

Keywords: trans-resveratrol, vasculogenesis and angiogenesis, stilbene, embryo growth, yolk-sac membrane

JOURNAL FREE ACCESS

2008 Volume 107 Issue 2 Pages 118-127

DOI https://doi.org/10.1254/jphs.FP0071876

Details

Abstract

Resveratrol is a stilbene compound found in grapes and other sources. In this study we examined the effects of trans-resveratrol (4.38 – 438 μM/implant) in the vasculogenesis of yolk-sac membranes and its capacity to improve chick embryo growth. High concentrations of the stilbene (43.8 – 438 μM) significantly inhibited early vessel formation, decreasing the percentage vitelline vessels of 3.5-day embryos by 50% compared to the control. In addition, basic fibroblast growth factor–stimulated vasculogenesis (140% of vessels as compared to control) was partially reversed by t-resveratrol (35% of inhibition) and treatments with cyclooxygenase inhibitors (acetylsalicylic acid and indomethacin) as well a protein-kinase C (PKC) activator (phorbol-12,13-dibutyrate) decreased the vessel number to 60%, 50%, and 44%, respectively. Treatments with t-resveratrol (4.38 – 43.8 μM/implant) significantly increased the body length of embryos incubated in vitro uncoupled from any impairment in the body shape or detectable embryotoxic effect. We suggest that the antivasculogenic activity and the enhancement in embryonic growth promoted by non acute treatments with t-resveratrol were, at least in part, due to PKC inhibition. We suggest that t-resveratrol can be usable not only as a reliable functional nutriment, but also is useful for the development of prophylactic and/or therapeutic agents for treatment of angiogenic-degenerative diseases.

Corresponding author

Register with J-STAGE for free!