Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification

Janisara Rudeeaneksin; Supranee Bunchoo; Sopa Srisungngam; Pathom Sawanpanyalert; Sawet Chamnangrom; Atipa Kamolwat; Porntip Thanasripakdeekul; Tooru Taniguchi; Chie Nakajima; Yasuhiko Suzuki; Benjawan Phetsuksiri

doi:10.7883/yoken.65.306

Original Article

Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification

Janisara Rudeeaneksin, Supranee Bunchoo, Sopa Srisungngam, Pathom Sawanpanyalert, Sawet Chamnangrom, Atipa Kamolwat, Porntip Thanasripakdeekul, Tooru Taniguchi, Chie Nakajima, Yasuhiko Suzuki, Benjawan Phetsuksiri

Author information

Janisara Rudeeaneksin
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Supranee Bunchoo
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Sopa Srisungngam
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Pathom Sawanpanyalert
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
Sawet Chamnangrom
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
Atipa Kamolwat
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
Porntip Thanasripakdeekul
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
Tooru Taniguchi
Research Collaboration Center for Emerging and Re-emerging Diseases, Osaka University
Chie Nakajima
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
Yasuhiko Suzuki
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
JICA/JST-SATREPS
Benjawan Phetsuksiri
National Institute of Health, Department of Medical Sciences, Ministry of Public Health

JOURNAL FREE ACCESS

2012 Volume 65 Issue 4 Pages 306-311

DOI https://doi.org/10.7883/yoken.65.306

Details

Abstract

Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex.

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