Development of an embryo-based genotyping system for efficient sire bleeding

Abstract

An embryo-based genotyping enables prenatal diagnosis of genes or markers for economically important traits or genetic disorders, and establishment of an embryo-based genotyping system is hoped to contribute efficiently selecting bulls as sires. We performed the embryo-based genotyping for CL-16 and SREBP-1 that are causative gene for CL-16 deficiency and fatty acid composition-related gene, respectively. In-vitro embryos were produced from Ovum Pick Up (OPU). Cells detachment was performed on 5 days after an in-vitro fertilization. To create nuclear transplant embryos for progeny(clone)test, these detached cells were used as a donor. We carried out genetic screening using developmental arrest embryos on 5-6 days after the nuclear transplant. The genotypes for CL-16 and SREBP-1 were successfully determined with the nuclear transplant embryo, and the genotypes determined by postnatal test were same as one by prenatal test. It was suggested that we will be able to get prenatally genotypic information of the candidate sire, and select effectively the sires harboring genotypes associated with the traits of interest.