2012 Volume 5 Issue 1 Pages 14-20
Chemotherapy and radiotherapy are performed for the treatment of cancer in children, especially leukemia. But, the side effects of these tr eatments are a problem of continuing concern, and improving the patient's quality of life (QOL) following successful treatment remains a challenge. Especially with radiation therapy, disorders of reproductive function are considerable, and cases of inferti lity are extremely high. Currently, research on the cryopreservation of ovarian tissue i s being energetically pursued, but because of factors such as reduction in number of egg cells and physical disruption due to tissue fragmentation, the successful fertiliz ation rate of the thawed ovum has been extremely low. Accordingly, cryopreservation of t he ovaries as an entire organ has been attempted, but this has yielded little success. This study focused on a method for cryopreservation through the application of sup ercooling under a variable magnetic field, and the development of a freezing syste m allowing for an arbitrary change in frequency of magnetic field applied to the specimen. To confirm the interrelation between a variable magnetic field and supercooling, physiological saline solution was frozen under a variable magnetic field, and the pro gress and stabilization of supercooling was verified for magnetic field frequencies from 200 to 200 kHz. Additionally, ovaries were frozen under magnetic field, and histological assessment of the tissue was performed.