Rapid Diagnostic Test vs. Real-Time PCR for Rapid Diagnosis of Infection with Adenovirus and Respiratory Syncytial Virus

Abstract

A new commercial rapid diagnostic test for 10-min for adenovirus (ADV) and respiratory syncytial virus (RSV) is now available for rapid diagnosis. The sensitivity and the specificity of these rapid diagnostic tests were compared to real-time PCR which is a “golden standard” for detecting ADV and RSV. Here, we assessed the 150 of real-time PCRpositive samples for ADV and 100 for RSV, and 81 (54%) and 63 (63%) of them were positive with rapid diagnostic test, respectively. The sensitivity of the rapid diagnostic test was 54% for ADV and 63.0% for RSV. Accordingly, the samples showing over 30 Ct in real-time PCR were detected by the ADV rapid diagnostic test and over 24 Ct by the RSV rapid diagnostic test. These results indicated that these test kits performed well enough to be used for rapid diagnosis of patients infected with ADV and RSV after 10- min with simple step.