Abstract
The aims of this study were to estimate the thickness and structure of hybrid layer formed with a self-etching bonding agent (AQ Bond) by SEM and TEM observations.
Various chemical modifications on specimens were useful for the observation of both SEM and TEM to study the hybrid layer. Less than 1μm of hybrid layer was discerned in TEM although it could not be observed clearly by SEM. According TEM observations, a demineralized dentin and resin-reinforced dentin were discernible in the hybrid layer. But if larger amount of bonding agent was placed on the prepared surface and cured, the demineralized smear layer was identified in the bonding layer and it could be adverse effects on the bonding.
It was concluded that smear must be scattered during the application of the self-etching bonding agent.
